cDNA Clone YGL082W

• Plasmid: pGEX6P1 YGL082W E27P C218S
• Parent Plasmid: pGEX6P1
• Expressed: GST YGL082W E27P C218S
• Genbank: NM_001180947
• Tag: GST

Sequence of Insert

ctcgagATGGATGTTACATTTCTAACCAAAAATGTTCAAATAAATGGCACTCAGTTTAAAATATTACTTCAAAATGGGCAAGGTCCATGCGCGCTAATTGCACTAGCTAACGTATTATTGATATCACCTGCCCATGCTCGCTATGCCCAAGAAATTTCCAGACTAGTGAGAGGCAAGGAAACTGTTACTTTAAACGAACTTGTGCAAACTCTAGCCGACATGGGCGTTCAAAATCCGAATGGTACAGATGTGGACAAACAACAGCTACTCCAAATCCTACCACAATTATATAGCGGATTGAACATCAACCCAGAATTTAACGGATCTTTTGAGGACGGCGTAGAAATGTCGATTTTCAGGCTCTACAATGTCGGCATCGTTCATGGATGGATAATAGATGGGGACAATGATCCGAACTCTTACGAACATGTGTCCAAATATTCTTATATGGGCGCGCAAAAAGTTTTGGTACAGTCGTATGAGATCCAAAAGAATAATGCCCAATTTGAAAACAGCGAGCAAATCCAATCTGATGCACCTTACTTAAAATCGTTTCTGGCAAGATCTGCCACACAATTGACTGAATATGGTCTAACTCATCTCAGAGAAATTTTAGTAGAAAGATCGTATGCCGTGCTTTTTAGAAATGATCACTTTAGCACATTATATAAAAACAATGGCGAATTGTTTACTTTGGTCACGGATCCTACTTACAGAAATCGCAAGGACATAAACTGGCAGTCGCTTAAATCCGTGAATGGATCGCAGGATTCTTACTACACGGGCAACTTCATTCCCACAAGTCTGGAAAGAACAGAAACTACTGCTACCGGGCAAAATGAGTCCTACATTTCTAACCCATTCAGTGACCAGAATACAGGGCATGTAACTAGCAACCAAGTTAATAGTGGAGCAAGCGGTGTTCAACAAATCGAAGATGATGAGGAACTAGCTAGGAGGTTACAGGAACAAGAAGATATGCGTGCGGCAAATAACATGCAAAATGGTTATGCTAATAACGGTAGAAATCATCAAAGGGAAAGGTTTGAAAGACCTGAAAAAAATTCAAAGAAAAACAAGTTCCTTCCCTTTAATGGGAGTAATAAAGAAAAGAAAAGAGACAAGTTGAAAAAAAACTGTGTAATTATGTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLLGSPNSRVEMDVTFLTKNVQINGTQFKILLQNGQGPCALIALANVLLISPAHARYAQEISRLVRGKETVTLNELVQTLADMGVQNPNGTDVDKQQLLQILPQLYSGLNINPEFNGSFEDGVEMSIFRLYNVGIVHGWIIDGDNDPNSYEHVSKYSYMGAQKVLVQSYEIQKNNAQFENSEQIQSDAPYLKSFLARSATQLTEYGLTHLREILVERSYAVLFRNDHFSTLYKNNGELFTLVTDPTYRNRKDINWQSLKSVNGSQDSYYTGNFIPTSLERTETTATGQNESYISNPFSDQNTGHVTSNQVNSGASGVQQIEDDEELARRLQEQEDMRAANNMQNGYANNGRNHQRERFERPEKNSKKNKFLPFNGSNKEKKRDKLKKNCVIM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU47722
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please make the point mutation from glutamate to proline on position 27 in the YGL082W C218S mutant backbone (DU 47603)