cDNA Clone BCAS2

• Synonyms: DAM1; Snt309; SPF27
• Plasmid: pETDuet 6His BCAS2/3FLAG PRP19 P41A
• Parent Plasmid: pETDuet1
• Expressed: 6His BCAS2
• Genbank: NM_005872.2 GI:49472833
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGATGGCGGGCACAGGTTTGGTGGCTGGAGAGGTTGTGGTGGATGCGCTGCCGTATTTTGATCAAGGTTATGAAGCCCCTGGTGTGCGGGAAGCGGCTGCAGCGCTGGTGGAGGAGGAAACTCGCAGATACCGACCTACTAAGAACTACCTGAGCTACCTGACAGCCCCGGATTATTCTGCCTTTGAAACTGACATAATGAGAAATGAATTTGAAAGACTGGCTGCTCGACAACCAATTGAATTGCTCAGTATGAAACGATATGAGCTTCCAGCCCCCTCCTCTGGTCAAAAAAATGACATTACTGCATGGCAAGAATGTGTAAACAATTCTATGGCCCAGTTAGAGCATCAAGCAGTTAGAATTGAGAATCTGGAACTAATGTCACAGCATGGATGTAATGCCTGGAAAGTATACAATGAAAATCTAGTTCATATGATTGAACACGCACAGAAGGAACTTCAGAAGTTAAGAAAACATATTCAAGATTTAAACTGGCAGAGAAAGAACATGCAACTCACAGCTGGATCTAAATTGAGAGAAATGGAGTCAAATTGGGTATCCCTGGTCAGTAAGAATTATGAGATTGAACGGACTATTGTTCAGCTAGAAAATGAAATCTATCAAATTAAGCAGCAACATGGAGAGGCAAACAAAGAAAACATCCGGCAAGACTTCTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSQDPMAGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYRPTKNYLSYLTAPDYSAFETDIMRNEFERLAARQPIELLSMKR
YELPAPSSGQKNDITAWQECVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLREMESNWV
SLVSKNYEIERTIVQLENEIYQIKQQHGEANKENIRQDF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU50721
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 3FLAG PRP19 cloned BamHI+1/XhoI into BglII/XhoI of DU50692 (pETDuet 6His BCAS2), followed by mutagenesis of DU50713 to create PRP19 P41A.