cDNA Clone ERCC5

• Synonyms: COFS3; ERCM2; UVDR; XPG; XPGC
• Plasmid: pET15 6His MBP TEV ERCC5
• Parent Plasmid: pET15 6HIS MBP DU
• Expressed: 6His MBP TEV ERCC5
• Genbank: NM_000123.3 GI:324715039 with one silent change
• Tag: 6His
• Tag: MBP
• Species: Human

Sequence of Insert

GCGGCCGCGATGGGGGTCCAGGGGCTCTGGAAGCTGCTGGAGTGCTCCGGGCGGCAGGTCAGCCCCGAAGCGCTGGAAGGGAAGATCCTGGCTGTTGATATTAGCATTTGGTTAAACCAAGCACTTAAAGGAGTCCGGGATCGCCACGGGAACTCAATAGAAAATCCTCATCTTCTCACTTTGTTTCATCGGCTCTGCAAACTCTTATTTTTTCGAATTCGTCCTATTTTTGTGTTTGATGGGGATGCTCCACTATTGAAGAAACAGACTTTGGTGAAGAGAAGGCAGAGAAAGGACTTAGCGTCCAGTGACTCCAGGAAAACGACAGAGAAGCTTCTGAAAACATTTTTGAAAAGACAAGCCATCAAAACTGCCTTCAGAAGCAAAAGAGATGAAGCACTACCCAGTCTTACCCAAGTTCGAAGAGAAAACGACCTCTATGTTTTGCCTCCTTTACAAGAGGAAGAAAAACACAGTTCAGAAGAGGAAGATGAAAAAGAATGGCAAGAAAGAATGAATCAAAAACAAGCATTACAGGAAGAGTTCTTTCATAATCCTCAAGCGATAGATATTGAGTCTGAGGACTTCAGCAGCCTGCCCCCTGAAGTAAAGCATGAAATCTTGACTGATATGAAAGAGTTCACCAAGCGCAGAAGAACATTATTTGAAGCAATGCCAGAGGAGTCTGATGACTTTTCACAGTACCAACTCAAAGGCTTGCTTAAAAAGAACTATCTGAACCAGCATATAGAACATGTCCAAAAGGAAATGAATCAGCAACATTCAGGACACATCCGAAGGCAGTATGAAGATGAAGGGGGCTTTCTGAAGGAGGTAGAGTCAAGGAGAGTGGTCTCTGAAGACACTTCACATTACATCTTGATAAAAGGTATTCAAGCTAAGACAGTTGCAGAAGTGGATTCAGAGTCTCTTCCTTCTTCCAGCAAAATGCACGGCATGTCTTTTGACGTGAAGTCATCTCCATGTGAAAAACTGAAGACAGAGAAAGAGCCTGATGCTACCCCTCCTTCTCCAAGAACTTTACTAGCTATGCAAGCTGCCCTGCTGGGAAGTAGCTCAGAAGAGGAGCTGGAGAGTGAAAATCGAAGGCAGGCCCGTGGGAGGAACGCACCTGCTGCTGTAGACGAAGGCTCCATATCACCCCGGACTCTTTCAGCCATTAAGAGAGCTCTTGACGATGACGAAGATGTAAAAGTGTGTGCTGGGGATGATGTGCAGACGGGAGGGCCAGGAGCAGAAGAAATGCGTATAAACAGCTCCACCGAGAACAGTGATGAAGGACTTAAAGTGAGAGATGGAAAAGGAATACCGTTTACTGCAACACTTGCGTCATCTAGTGTGAACTCTGCAGAGGAGCACGTAGCCAGCACTAATGAGGGGAGAGAGCCCACAGACTCAGTTCCAAAAGAACAAATGTCACTTGTTCACGTGGGGACTGAAGCCTTTCCGATAAGTGATGAGTCTATGATTAAGGACAGAAAAGATCGGCTGCCTCTGGAGAGTGCAGTGGTTAGACATAGTGACGCACCTGGGCTCCCGAATGGAAGGGAACTGACACCGGCATCTCCAACTTGTACAAATTCTGTGTCAAAGAATGAAACACATGCTGAAGTGCTTGAGCAGCAGAACGAACTTTGCCCATATGAGAGTAAATTCGATTCTTCTCTTCTTTCAAGTGATGATGAAACAAAATGTAAACCGAATTCTGCTTCTGAAGTCATTGGCCCTGTCAGTTTGCAAGAAACAAGTAGCATAGTAAGTGTCCCTTCAGAGGCAGTAGATAATGTGGAAAATGTGGTGTCATTTAATGCTAAAGAGCATGAGAATTTTCTGGAAACCATCCAAGAACAGCAGACCACTGAATCTGCAGGCCAGGATTTAATTTCCATTCCAAAGGCCGTGGAACCAATGGAAATTGACTCGGAAGAAAGTGAATCTGATGGAAGTTTCATTGAAGTGCAAAGTGTGATTAGTGATGAGGAACTTCAAGCAGAATTCCCTGAAACTTCCAAACCTCCCTCAGAACAAGGCGAAGAGGAACTGGTAGGAACTAGGGAGGGAGAAGCCCCTGCTGAGTCCGAGAGCCTCCTGAGGGACAACTCTGAGAGGGACGACGTGGATGGTGAGCCACAGGAAGCTGAGAAAGATGCGGAAGATTCGCTCCATGAATGGCAAGATATTAATTTGGAGGAGTTGGAAACTCTGGAGAGCAACCTCTTAGCACAGCAGAATTCACTGAAAGCTCAAAAACAGCAGCAAGAACGGATCGCTGCTACTGTCACCGGACAGATGTTCCTGGAAAGCCAGGAACTCCTGCGCCTGTTCGGCATTCCCTACATCCAGGCTCCCATGGAAGCAGAGGCGCAGTGCGCCATCCTGGACCTGACTGATCAGACTTCCGGAACCATCACTGATGACAGTGATATCTGGCTGTTTGGAGCGCGGCATGTCTATAGAAACTTTTTTAATAAAAACAAGTTTGTAGAATATTATCAATATGTGGACTTTCACAATCAATTGGGATTGGACCGGAATAAGTTAATAAATTTGGCTTATTTGCTTGGAAGTGATTATACCGAAGGAATACCAACTGTGGGTTGTGTAACCGCCATGGAAATTCTCAATGAATTCCCTGGGCATGGCCTGGAACCTCTCCTAAAATTCTCAGAATGGTGGCATGAAGCTCAAAAAAATCCAAAGATAAGACCTAATCCTCATGACACCAAAGTGAAAAAAAAATTACGGACATTGCAACTCACCCCTGGCTTTCCTAACCCAGCTGTTGCCGAGGCCTACCTCAAACCCGTGGTGGATGACTCGAAGGGATCCTTTCTGTGGGGGAAACCTGATCTCGACAAAATTAGAGAATTTTGTCAGCGGTATTTCGGCTGGAACAGAACGAAGACAGATGAATCTCTGTTTCCTGTATTAAAGCAACTCGATGCCCAGCAGACACAGCTCCGAATTGATTCCTTCTTTAGATTAGCACAACAGGAGAAAGAAGATGCTAAACGTATTAAGAGCCAGAGACTAAACAGAGCTGTGACATGTATGCTAAGGAAAGAGAAAGAAGCAGCAGCCAGCGAAATAGAAGCAGTTTCTGTTGCCATGGAGAAAGAATTTGAGCTACTTGATAAGGCAAAACGAAAAACCCAGAAGAGAGGCATAACAAATACCTTAGAAGAGTCATCAAGCCTGAAAAGAAAGAGGCTTTCAGATTCTAAACGAAAGAATACATGCGGTGGATTTTTGGGGGAGACCTGCCTCTCAGAATCATCTGATGGATCTTCAAGTGAAGATGCTGAAAGTTCATCTTTAATGAATGTACAAAGGAGAACAGCTGCGAAAGAGCCAAAAACCAGTGCTTCAGATTCGCAGAACTCAGTGAAGGAAGCTCCCGTGAAGAATGGAGGTGCGACCACCAGCAGCTCTAGTGATAGTGATGACGATGGAGGGAAAGAGAAGATGGTCCTCGTGACCGCCAGATCTGTGTTTGGGAAGAAAAGAAGGAAACTAAGACGTGCGAGGGGAAGAAAAAGGAAAACCTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHGSSMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQ
DKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNA
GAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLT
DEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGENLY
FQGHMGSPEFPGRAAAMGVQGLWKLLECSGRQVSPEALEGKILAVDISIWLNQALKGVRDRHGNSIENPHLLTLFHRLCKLLFFRIRPIFVFDGDAPLLK
KQTLVKRRQRKDLASSDSRKTTEKLLKTFLKRQAIKTAFRSKRDEALPSLTQVRRENDLYVLPPLQEEEKHSSEEEDEKEWQERMNQKQALQEEFFHNPQ
AIDIESEDFSSLPPEVKHEILTDMKEFTKRRRTLFEAMPEESDDFSQYQLKGLLKKNYLNQHIEHVQKEMNQQHSGHIRRQYEDEGGFLKEVESRRVVSE
DTSHYILIKGIQAKTVAEVDSESLPSSSKMHGMSFDVKSSPCEKLKTEKEPDATPPSPRTLLAMQAALLGSSSEEELESENRRQARGRNAPAAVDEGSIS
PRTLSAIKRALDDDEDVKVCAGDDVQTGGPGAEEMRINSSTENSDEGLKVRDGKGIPFTATLASSSVNSAEEHVASTNEGREPTDSVPKEQMSLVHVGTE
AFPISDESMIKDRKDRLPLESAVVRHSDAPGLPNGRELTPASPTCTNSVSKNETHAEVLEQQNELCPYESKFDSSLLSSDDETKCKPNSASEVIGPVSLQ
ETSSIVSVPSEAVDNVENVVSFNAKEHENFLETIQEQQTTESAGQDLISIPKAVEPMEIDSEESESDGSFIEVQSVISDEELQAEFPETSKPPSEQGEEE
LVGTREGEAPAESESLLRDNSERDDVDGEPQEAEKDAEDSLHEWQDINLEELETLESNLLAQQNSLKAQKQQQERIAATVTGQMFLESQELLRLFGIPYI
QAPMEAEAQCAILDLTDQTSGTITDDSDIWLFGARHVYRNFFNKNKFVEYYQYVDFHNQLGLDRNKLINLAYLLGSDYTEGIPTVGCVTAMEILNEFPGH
GLEPLLKFSEWWHEAQKNPKIRPNPHDTKVKKKLRTLQLTPGFPNPAVAEAYLKPVVDDSKGSFLWGKPDLDKIREFCQRYFGWNRTKTDESLFPVLKQL
DAQQTQLRIDSFFRLAQQEKEDAKRIKSQRLNRAVTCMLRKEKEAAASEIEAVSVAMEKEFELLDKAKRKTQKRGITNTLEESSSLKRKRLSDSKRKNTC
GGFLGETCLSESSDGSSSEDAESSSLMNVQRRTAAKEPKTSASDSQNSVKEAPVKNGGATTSSSSDSDDDGGKEKMVLVTARSVFGKKRRKLRRARGRKR
KT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU50833
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee