cDNA Clone SELO

• Plasmid: pcDNA5 FRT/TO SELO 3FLAG D348A
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: SELO 3FLAG D348A
• Genbank: Q9BVL4
• Tag: 3FLAG
• Species: Human

Sequence of Insert

ggagatctGCCACCATGGCCGTATACAGGGCAGCGCTCGGGGCTTCGCTCGCGGCTGCCCGACTCTTGCCCCTCGGTCGCTGTTCCCCGTCGCCGGCGCC
CCGCTCTACGTTGTCGGGCGCCGCCATGGAGCCCGCGCCTCGCTGGCTGGCGGGGCTGCGCTTCGACAACCGCGCCCTGCGCGCCCTGCCCGTGGAGGCG
CCGCCGCCCGGTCCCGAGGGCGCCCCGTCCGCGCCGCGGCCCGTGCCCGGGGCCTGCTTCACCCGCGTGCAGCCCACCCCGCTGCGGCAGCCGCGCCTCG
TGGCGCTGTCAGAGCCCGCGCTGGCGTTGCTGGGCCTGGGCGCGCCGCCCGCGCGCGAGGCCGAGGCCGAGGCCGCGCTGTTCTTCAGCGGCAACGCGCT
CCTGCCGGGCGCCGAGCCCGCCGCGCACTGCTACTGCGGCCACCAATTCGGCCAGTTCGCCGGGCAGCTGGGCGACGGCGCCGCCATGTACCTGGGCGAG
GTGTGCACGGCGACCGGCGAGCGCTGGGAGCTGCAGCTCAAGGGCGCCGGGCCCACGCCCTTCTCCAGACAGGCCGACGGTCGCAAGGTCCTACGGTCAA
GCATCCGGGAGTTTCTATGCAGCGAAGCCATGTTCCACCTGGGAGTCCCCACCACACGGGCCGGCGCCTGCGTCACGTCCGAGTCCACGGTGGTGCGCGA
CGTGTTCTATGATGGTAATCCCAAATATGAACAATGCACGGTTGTGTTGCGTGTAGCTTCCACTTTCATAAGGTTTGGATCCTTTGAGATTTTTAAGTCT
GCAGATGAGCACACAGGGCGTGCAGGCCCCAGCGTGGGGAGGAACGACATTCGAGTGCAGCTGCTCGACTATGTCATCAGCTCCTTTTACCCCGAGATCC
AGGCTGCTCATGCCAGCGACAGCGTGCAGAGAAATGCTGCCTTCTTCCGGGAGGTGACGCGGCGCACGGCGCGGATGGTGGCCGAGTGGCAGTGTGTGGG
CTTCTGCCACGGCGTGCTCAACACCGACAACATGAGCATCCTGGGGCTCACCATCGCCTACGGGCCCTTTGGCTTCCTGGACAGGTACGACCCCGACCAC
GTGTGCAATGCCTCCGACAACACCGGCCGCTACGCGTACAGCAAGCAGCCCGAGGTGTGCAGGTGGAACCTGCGGAAGCTGGCCGAGGCCCTGCAGCCGG
AACTGCCCCTGGAGCTGGGGGAGGCCATCCTGGCCGAGGAGTTTGACGCCGAGTTCCAAAGGCACTACCTGCAGAAGATGCGCAGGAAGCTGGGCCTCGT
GCAGGTGGAGCTGGAGGAAGACGGGGCGCTGGTGTCCAAGCTCCTGGAGACCATGCATCTGACCGGTGCCGACTTCACAAACACCTTCTACTTGCTGAGC
TCCTTCCCAGTGGAGCTAGAGTCGCCAGGCCTGGCGGAATTCCTGGCCAGGCTGATGGAGCAGTGTGCCTCCCTGGAGGAGCTGAGGCTGGCCTTCCGGC
CCCAGATGGATCCCCGGCAGCTATCCATGATGCTGATGCTGGCGCAGTCAAACCCGCAGCTGTTCGCGCTTATGGGCACCCGGGCAGGCATCGCCAGGGA
GCTGGAGCGTGTGGAGCAGCAGTCTCGGCTGGAGCAGCTGAGTGCGGCAGAGCTGCAGAGCAGGAACCAGGGCCACTGGGCTGACTGGCTACAGGCGTAC
AGAGCCCGGCTGGACAAGGACCTGGAAGGCGCTGGGGACGCTGCCGCCTGGCAGGCTGAGCACGTGCGCGTGATGCACGCCAACAACCCGAAGTACGTGC
TGAGGAACTACATCGCGCAGAATGCCATCGAGGCTGCCGAGCGCGGGGACTTCTCAGAGGTGCGGCGGGTGCTGAAACTACTGGAGACCCCTTACCACTG
CGAGGCGGGGGCCGCCACAGACGCCGAGGCCACGGAAGCCGACGGGGCGGACGGCAGGCAGCGCTCCTACAGCAGTAAGCCCCCGCTCTGGGCAGCAGAA
CTGTGCGTGACATGATCTTCGgcggccgc

Amino Acid Sequence

MAVYRAALGASLAAARLLPLGRCSPSPAPRSTLSGAAMEPAPRWLAGLRFDNRALRALPVEAPPPGPEGAPSAPRPVPGACFTRVQPTPLRQPRLVALSE
PALALLGLGAPPAREAEAEAALFFSGNALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTATGERWELQLKGAGPTPFSRQADGRKVLRSSIREF
LCSEAMFHLGVPTTRAGACVTSESTVVRDVFYDGNPKYEQCTVVLRVASTFIRFGSFEIFKSADEHTGRAGPSVGRNDIRVQLLDYVISSFYPEIQAAHA
SDSVQRNAAFFREVTRRTARMVAEWQCVGFCHGVLNTDNMSILGLTIAYGPFGFLDRYDPDHVCNASDNTGRYAYSKQPEVCRWNLRKLAEALQPELPLE
LGEAILAEEFDAEFQRHYLQKMRRKLGLVQVELEEDGALVSKLLETMHLTGADFTNTFYLLSSFPVELESPGLAEFLARLMEQCASLEELRLAFRPQMDP
RQLSMMLMLAQSNPQLFALMGTRAGIARELERVEQQSRLEQLSAAELQSRNQGHWADWLQAYRARLDKDLEGAGDAAAWQAEHVRVMHANNPKYVLRNYI
AQNAIEAAERGDFSEVRRVLKLLETPYHCEAGAATDAEATEADGADGRQRSYSSKPPLWAAELCVT*SSAADYMDDDDKDYKDDDDKDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU51577
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: SELO has a stop codon 3aa from the actual stop which appears to be read through. Lee et al. Characterization of mammalian Selenoprtein O: A Redox-active mitochondrial protein. PLos One 9(4): e95518. doi:10.1371/journal.pone.0095518