cDNA Clone CDKL5

• Synonyms: EIEE2; ISSX; STK9
• Plasmid: pFastbac Dual GST 6P1 CDKL5
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST 3C CDKL5
• Genbank: NM_003159.2 GI:83367066*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAAGATTCCTAACATTGGTAATGTGATGAATAAATTTGAGAT
CCTTGGGGTTGTAGGTGAAGGAGCCTATGGAGTTGTACTTAAATGCAGAC
ACAAGGAAACACATGAAATTGTGGCGATCAAGAAATTCAAGGACAGTGAA
GAAAATGAAGAAGTCAAAGAAACGACTTTACGAGAGCTTAAAATGCTTCG
GACTCTCAAGCAGGAAAACATTGTGGAGTTGAAGGAAGCATTTCGTCGGA
GGGGAAAGTTGTACTTGGTGTTTGAGTATGTTGAAAAAAATATGCTCGAA
TTGCTGGAAGAAATGCCAAATGGAGTTCCACCTGAGAAAGTAAAAAGCTA
CATCTATCAGCTAATCAAGGCTATTCACTGGTGCCATAAGAATGATATTG
TCCATCGAGATATAAAACCAGAAAATCTCTTAATCAGCCACAATGATGTC
CTAAAACTGTGTGACTTTGGTTTTGCTCGTAATCTGTCAGAAGGCAATAA
TGCTAATTACACAGAGTACGTTGCCACCAGATGGTATCGGTCCCCAGAAC
TCTTACTTGGCGCTCCCTATGGAAAGTCCGTGGACATGTGGTCGGTGGGC
TGTATTCTTGGGGAGCTTAGCGATGGACAGCCTTTATTTCCTGGAGAAAG
TGAAATTGACCAACTTTTTACTATTCAGAAGGTGCTAGGACCACTTCCAT
CTGAGCAGATGAAGCTTTTCTACAGTAATCCTCGCTTCCATGGGCTCCGG
TTTCCAGCTGTTAACCATCCTCAGTCCTTGGAAAGAAGATACCTTGGAAT
TTTGAATAGTGTTCTACTTGACCTAATGAAGAATTTACTGAAGTTGGACC
CAGCTGACAGATACTTGACAGAACAGTGTTTGAATCACCCTACATTTCAA
ACCCAGAGACTTCTGGATCGTTCTCCTTCAAGGTCAGCAAAAAGAAAACC
TTACCATGTGGAAAGCAGCACATTGTCTAATAGAAACCAAGCCGGCAAAA
GCACTGCTTTGCAGTCTCACCACAGATCTAACAGCAAGGACATCCAGAAC
CTGAGTGTAGGCCTGCCCCGGGCTGACGAAGGTCTCCCTGCCAATGAAAG
CTTCCTAAATGGAAACCTTGCTGGAGCTAGTCTTAGTCCACTGCACACCA
AAACCTACCAAGCAAGCAGCCAGCCTGGGTCTACCAGCAAAGATCTCACC
AACAACAACATACCACACCTTCTTAGCCCAAAAGAAGCCAAGTCAAAAAC
AGAGTTTGATTTTAATATTGACCCAAAGCCTTCAGAAGGCCCAGGGACAA
AGTACCTCAAGTCAAACAGCAGATCTCAGCAGAACCGCCACTCATTCATG
GAAAGCTCTCAAAGCAAAGCTGGGACACTGCAGCCCAATGAAAAGCAGAG
TCGGCATAGCTATATTGACACAATTCCCCAGTCCTCTAGGAGTCCCTCCT
ACAGGACCAAGGCCAAAAGCCATGGGGCACTGAGTGACTCCAAGTCTGTG
AGCAACCTTTCTGAAGCCAGGGCCCAAATTGCGGAGCCCAGTACCAGTAG
GTACTTCCCATCTAGCTGCTTAGACTTGAATTCTCCCACCAGCCCAACCC
CCACCAGACACAGTGACACGAGAACTTTGCTCAGCCCTTCTGGAAGAAAT
AACCGAAATGAGGGAACGCTGGACTCACGTCGAACCACAACCAGACATTC
TAAGACGATGGAGGAATTGAAGCTGCCGGAGCACATGGACAGTAGCCATT
CCCATTCACTGTCTGCACCTCACGAATCTTTTTCTTATGGACTGGGCTAC
ACCAGCCCCTTTTCTTCCCAGCAACGTCCTCATAGGCATTCTATGTATGT
GACCCGTGACAAAGTGAGAGCCAAGGGCTTGGATGGAAGCTTGAGCATAG
GGCAAGGGATGGCAGCTAGAGCCAACAGCCTGCAACTCTTGTCACCCCAG
CCTGGAGAACAGCTCCCTCCAGAGATGACTGTGGCAAGATCTTCGGTCAA
AGAGACCTCCAGAGAAGGCACCTCTTCCTTCCATACACGCCAGAAGTCTG
AGGGTGGAGTGTATCATGACCCACACTCTGATGATGGCACAGCCCCCAAA
GAAAATAGACACCTATACAATGACCCTGTGCCAAGGAGAGTTGGTAGCTT
TTACAGAGTGCCATCTCCACGTCCAGACAATTCTTTCCATGAAAATAATG
TGTCAACTAGAGTTTCTTCTCTACCATCAGAGAGCAGTTCTGGAACCAAC
CACTCAAAAAGACAACCAGCATTCGATCCATGGAAAAGTCCTGAAAATAT
TAGTCATTCAGAGCAACTCAAGGAAAAAGAGAAGCAAGGATTTTTCAGGT
CAATGAAAAAGAAAAAGAAGAAATCTCAAACAGTACCCAATTCCGACAGC
CCTGATCTTCTGACGTTGCAGAAATCCATTCATTCTGCTAGCACTCCAAG
CAGCAGACCAAAGGAGTGGCGCCCCGAGAAGATCTCAGATCTGCAGACCC
AAAGCCAGCCATTAAAATCACTGCGCAAGTTGTTACATCTCTCTTCGGCC
TCAAATCACCCGGCTTCCTCAGATCCCCGCTTCCAGCCCTTAACAGCTCA
ACAAACCAAAAATTCCTTCTCAGAAATTCGGATTCACCCCCTGAGCCAGG
CCTCTGGCGGGAGCAGCAACATCCGGCAGGAACCCGCACCGAAGGGCAGG
CCAGCCCTCCAGCTGCCAGACGGTGGATGTGATGGCAGAAGACAGAGACA
CCATTCTGGACCCCAAGATAGACGCTTCATGTTAAGGACGACAGAACAAC
AAGGAGAATACTTCTGCTGTGGTGACCCAAAGAAGCCTCACACTCCGTGC
GTCCCAAACCGAGCCCTTCATCGTCCAATCTCCAGTCCTGCTCCCTATCC
AGTACTCCAGGTCCGAGGCACTTCCATGTGCCCGACACTCCAGGTCCGAG
GCACTGATGCTTTCAGCTGCCCAACCCAGCAATCCGGGTTCTCTTTCTTC
GTGAGACACGTTATGAGGGAAGCCCTGATTCACAGGGCCCAGGTAAACCA
AGCTGCGCTCCTGACATACCATGAGAATGCGGCACTGACGGGCAAGTGAG
CGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMKIPNIGNVMNKFEILGVVGEGAYGVVLKCRHKETHEIVAIKKFKDSEENEEVKETTLRELKMLRTLKQ
ENIVELKEAFRRRGKLYLVFEYVEKNMLELLEEMPNGVPPEKVKSYIYQLIKAIHWCHKNDIVHRDIKPENLLISHNDVLKLCDFGFARNLSEGNNANYT
EYVATRWYRSPELLLGAPYGKSVDMWSVGCILGELSDGQPLFPGESEIDQLFTIQKVLGPLPSEQMKLFYSNPRFHGLRFPAVNHPQSLERRYLGILNSV
LLDLMKNLLKLDPADRYLTEQCLNHPTFQTQRLLDRSPSRSAKRKPYHVESSTLSNRNQAGKSTALQSHHRSNSKDIQNLSVGLPRADEGLPANESFLNG
NLAGASLSPLHTKTYQASSQPGSTSKDLTNNNIPHLLSPKEAKSKTEFDFNIDPKPSEGPGTKYLKSNSRSQQNRHSFMESSQSKAGTLQPNEKQSRHSY
IDTIPQSSRSPSYRTKAKSHGALSDSKSVSNLSEARAQIAEPSTSRYFPSSCLDLNSPTSPTPTRHSDTRTLLSPSGRNNRNEGTLDSRRTTTRHSKTME
ELKLPEHMDSSHSHSLSAPHESFSYGLGYTSPFSSQQRPHRHSMYVTRDKVRAKGLDGSLSIGQGMAARANSLQLLSPQPGEQLPPEMTVARSSVKETSR
EGTSSFHTRQKSEGGVYHDPHSDDGTAPKENRHLYNDPVPRRVGSFYRVPSPRPDNSFHENNVSTRVSSLPSESSSGTNHSKRQPAFDPWKSPENISHSE
QLKEKEKQGFFRSMKKKKKKSQTVPNSDSPDLLTLQKSIHSASTPSSRPKEWRPEKISDLQTQSQPLKSLRKLLHLSSASNHPASSDPRFQPLTAQQTKN
SFSEIRIHPLSQASGGSSNIRQEPAPKGRPALQLPDGGCDGRRQRHHSGPQDRRFMLRTTEQQGEYFCCGDPKKPHTPCVPNRALHRPISSPAPYPVLQV
RGTSMCPTLQVRGTDAFSCPTQQSGFSFFVRHVMREALIHRAQVNQAALLTYHENAALTGK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU50810
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_003159.2 CDS:; t996c, t2118c (both silent)