cDNA Clone CDKL5

• Synonyms: EIEE2; ISSX; STK9
• Plasmid: pBabeD puro GFP CDKL5
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP CDKL5
• Genbank: NM_003159.2 GI:83367066*
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGAAGATTCCTAACATTGGTAATGTGATGAATAAATTTGAGAT
CCTTGGGGTTGTAGGTGAAGGAGCCTATGGAGTTGTACTTAAATGCAGAC
ACAAGGAAACACATGAAATTGTGGCGATCAAGAAATTCAAGGACAGTGAA
GAAAATGAAGAAGTCAAAGAAACGACTTTACGAGAGCTTAAAATGCTTCG
GACTCTCAAGCAGGAAAACATTGTGGAGTTGAAGGAAGCATTTCGTCGGA
GGGGAAAGTTGTACTTGGTGTTTGAGTATGTTGAAAAAAATATGCTCGAA
TTGCTGGAAGAAATGCCAAATGGAGTTCCACCTGAGAAAGTAAAAAGCTA
CATCTATCAGCTAATCAAGGCTATTCACTGGTGCCATAAGAATGATATTG
TCCATCGAGATATAAAACCAGAAAATCTCTTAATCAGCCACAATGATGTC
CTAAAACTGTGTGACTTTGGTTTTGCTCGTAATCTGTCAGAAGGCAATAA
TGCTAATTACACAGAGTACGTTGCCACCAGATGGTATCGGTCCCCAGAAC
TCTTACTTGGCGCTCCCTATGGAAAGTCCGTGGACATGTGGTCGGTGGGC
TGTATTCTTGGGGAGCTTAGCGATGGACAGCCTTTATTTCCTGGAGAAAG
TGAAATTGACCAACTTTTTACTATTCAGAAGGTGCTAGGACCACTTCCAT
CTGAGCAGATGAAGCTTTTCTACAGTAATCCTCGCTTCCATGGGCTCCGG
TTTCCAGCTGTTAACCATCCTCAGTCCTTGGAAAGAAGATACCTTGGAAT
TTTGAATAGTGTTCTACTTGACCTAATGAAGAATTTACTGAAGTTGGACC
CAGCTGACAGATACTTGACAGAACAGTGTTTGAATCACCCTACATTTCAA
ACCCAGAGACTTCTGGATCGTTCTCCTTCAAGGTCAGCAAAAAGAAAACC
TTACCATGTGGAAAGCAGCACATTGTCTAATAGAAACCAAGCCGGCAAAA
GCACTGCTTTGCAGTCTCACCACAGATCTAACAGCAAGGACATCCAGAAC
CTGAGTGTAGGCCTGCCCCGGGCTGACGAAGGTCTCCCTGCCAATGAAAG
CTTCCTAAATGGAAACCTTGCTGGAGCTAGTCTTAGTCCACTGCACACCA
AAACCTACCAAGCAAGCAGCCAGCCTGGGTCTACCAGCAAAGATCTCACC
AACAACAACATACCACACCTTCTTAGCCCAAAAGAAGCCAAGTCAAAAAC
AGAGTTTGATTTTAATATTGACCCAAAGCCTTCAGAAGGCCCAGGGACAA
AGTACCTCAAGTCAAACAGCAGATCTCAGCAGAACCGCCACTCATTCATG
GAAAGCTCTCAAAGCAAAGCTGGGACACTGCAGCCCAATGAAAAGCAGAG
TCGGCATAGCTATATTGACACAATTCCCCAGTCCTCTAGGAGTCCCTCCT
ACAGGACCAAGGCCAAAAGCCATGGGGCACTGAGTGACTCCAAGTCTGTG
AGCAACCTTTCTGAAGCCAGGGCCCAAATTGCGGAGCCCAGTACCAGTAG
GTACTTCCCATCTAGCTGCTTAGACTTGAATTCTCCCACCAGCCCAACCC
CCACCAGACACAGTGACACGAGAACTTTGCTCAGCCCTTCTGGAAGAAAT
AACCGAAATGAGGGAACGCTGGACTCACGTCGAACCACAACCAGACATTC
TAAGACGATGGAGGAATTGAAGCTGCCGGAGCACATGGACAGTAGCCATT
CCCATTCACTGTCTGCACCTCACGAATCTTTTTCTTATGGACTGGGCTAC
ACCAGCCCCTTTTCTTCCCAGCAACGTCCTCATAGGCATTCTATGTATGT
GACCCGTGACAAAGTGAGAGCCAAGGGCTTGGATGGAAGCTTGAGCATAG
GGCAAGGGATGGCAGCTAGAGCCAACAGCCTGCAACTCTTGTCACCCCAG
CCTGGAGAACAGCTCCCTCCAGAGATGACTGTGGCAAGATCTTCGGTCAA
AGAGACCTCCAGAGAAGGCACCTCTTCCTTCCATACACGCCAGAAGTCTG
AGGGTGGAGTGTATCATGACCCACACTCTGATGATGGCACAGCCCCCAAA
GAAAATAGACACCTATACAATGACCCTGTGCCAAGGAGAGTTGGTAGCTT
TTACAGAGTGCCATCTCCACGTCCAGACAATTCTTTCCATGAAAATAATG
TGTCAACTAGAGTTTCTTCTCTACCATCAGAGAGCAGTTCTGGAACCAAC
CACTCAAAAAGACAACCAGCATTCGATCCATGGAAAAGTCCTGAAAATAT
TAGTCATTCAGAGCAACTCAAGGAAAAAGAGAAGCAAGGATTTTTCAGGT
CAATGAAAAAGAAAAAGAAGAAATCTCAAACAGTACCCAATTCCGACAGC
CCTGATCTTCTGACGTTGCAGAAATCCATTCATTCTGCTAGCACTCCAAG
CAGCAGACCAAAGGAGTGGCGCCCCGAGAAGATCTCAGATCTGCAGACCC
AAAGCCAGCCATTAAAATCACTGCGCAAGTTGTTACATCTCTCTTCGGCC
TCAAATCACCCGGCTTCCTCAGATCCCCGCTTCCAGCCCTTAACAGCTCA
ACAAACCAAAAATTCCTTCTCAGAAATTCGGATTCACCCCCTGAGCCAGG
CCTCTGGCGGGAGCAGCAACATCCGGCAGGAACCCGCACCGAAGGGCAGG
CCAGCCCTCCAGCTGCCAGACGGTGGATGTGATGGCAGAAGACAGAGACA
CCATTCTGGACCCCAAGATAGACGCTTCATGTTAAGGACGACAGAACAAC
AAGGAGAATACTTCTGCTGTGGTGACCCAAAGAAGCCTCACACTCCGTGC
GTCCCAAACCGAGCCCTTCATCGTCCAATCTCCAGTCCTGCTCCCTATCC
AGTACTCCAGGTCCGAGGCACTTCCATGTGCCCGACACTCCAGGTCCGAG
GCACTGATGCTTTCAGCTGCCCAACCCAGCAATCCGGGTTCTCTTTCTTC
GTGAGACACGTTATGAGGGAAGCCCTGATTCACAGGGCCCAGGTAAACCA
AGCTGCGCTCCTGACATACCATGAGAATGCGGCACTGACGGGCAAGTGAG
CGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMKIPNIGNVMNKFEILGVVGEGAYGVVLKCRHKETHEIVAIKKFKDSEENEEVKE
TTLRELKMLRTLKQENIVELKEAFRRRGKLYLVFEYVEKNMLELLEEMPNGVPPEKVKSYIYQLIKAIHWCHKNDIVHRDIKPENLLISHNDVLKLCDFG
FARNLSEGNNANYTEYVATRWYRSPELLLGAPYGKSVDMWSVGCILGELSDGQPLFPGESEIDQLFTIQKVLGPLPSEQMKLFYSNPRFHGLRFPAVNHP
QSLERRYLGILNSVLLDLMKNLLKLDPADRYLTEQCLNHPTFQTQRLLDRSPSRSAKRKPYHVESSTLSNRNQAGKSTALQSHHRSNSKDIQNLSVGLPR
ADEGLPANESFLNGNLAGASLSPLHTKTYQASSQPGSTSKDLTNNNIPHLLSPKEAKSKTEFDFNIDPKPSEGPGTKYLKSNSRSQQNRHSFMESSQSKA
GTLQPNEKQSRHSYIDTIPQSSRSPSYRTKAKSHGALSDSKSVSNLSEARAQIAEPSTSRYFPSSCLDLNSPTSPTPTRHSDTRTLLSPSGRNNRNEGTL
DSRRTTTRHSKTMEELKLPEHMDSSHSHSLSAPHESFSYGLGYTSPFSSQQRPHRHSMYVTRDKVRAKGLDGSLSIGQGMAARANSLQLLSPQPGEQLPP
EMTVARSSVKETSREGTSSFHTRQKSEGGVYHDPHSDDGTAPKENRHLYNDPVPRRVGSFYRVPSPRPDNSFHENNVSTRVSSLPSESSSGTNHSKRQPA
FDPWKSPENISHSEQLKEKEKQGFFRSMKKKKKKSQTVPNSDSPDLLTLQKSIHSASTPSSRPKEWRPEKISDLQTQSQPLKSLRKLLHLSSASNHPASS
DPRFQPLTAQQTKNSFSEIRIHPLSQASGGSSNIRQEPAPKGRPALQLPDGGCDGRRQRHHSGPQDRRFMLRTTEQQGEYFCCGDPKKPHTPCVPNRALH
RPISSPAPYPVLQVRGTSMCPTLQVRGTDAFSCPTQQSGFSFFVRHVMREALIHRAQVNQAALLTYHENAALTGK*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pBabeD puro GFP CDKL577.91 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU50811
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_003159.2 CDS:; t996c, t2118c (both silent)
• Low Temp: No