cDNA Clone HUWE1

• Synonyms: ARF-BP1; HECTH9; HSPC272; Ib772; LASU1; MULE; URE-B1; UREB1
• Plasmid: pCAGGS puro HA Huwe1 M1-S1000
• Parent Plasmid: pCAGGS puro HA
• Expressed: HA Huwe1 M1-S1000
• Genbank: NM_031407.6 GI:531034782 with mutations -see notes
• Tag: HA
• Species: Human

Sequence of Insert

AGATCTATGAAAGTAGACAGGACTAAACTGAAGAAGACACCTACTGAGGCTCCTGCAGACTGCAGAGCCTTAATAGACAAACTCAAAGTTTGTAATGATGAGCAACTTCTCTTGGAACTGCAGCAGATCAAAACATGGAACATTGGAAAGTGCGAGTTATATCACTGGGTGGACCTGTTGGACCGCTTCGATGGAATACTGGCAGATGCTGGACAGACAGTGGAGAATATGTCATGGATGCTCGTATGTGATAGGCCAGAAAGAGAGCAACTGAAAATGCTTCTCTTGGCTGTGTTGAACTTCACAGCCTTGCTCATTGAGTACAGCTTTTCCCGGCATCTGTACAGTTCCATAGAGCATTTGACAACTTTATTGGCTTCCTCTGATATGCAAGTGGTGCTGGCAGTCCTCAATCTCCTATATGTATTTAGCAAAAGATCAAACTACATCACTCGTCTGGGATCTGACAAGAGGACCCCGCTGCTAACTCGGCTACAACATTTGGCAGAGAGCTGGGGTGGAAAGGAGAATGGCTTTGGACTTGCAGAATGTTGCAGAGACTTGCATATGATGAAATATCCACCCAGTGCAACTACACTACACTTTGAATTCTACGCAGATCCTGGGGCCGAGGTCAAAATTGAGAAAAGGACAACTAGTAACACACTACATTATATTCACATAGAGCAACTTGACAAGATTTCAGAAAGCCCTTCTGAAATCATGGAATCTCTTACCAAAATGTACAGCATTCCTAAGGATAAGCAGATGCTGTTATTTACACACATACGACTGGCCCATGGCTTTTCTAATCACAGGAAGCGATTGCAGGCAGTTCAGGCCAGACTGCATGCAATATCTATATTAGTGTATTCCAATGCCTTGCAGGAATCAGCAAACAGTATCTTGTATAATGGCTTGATAGAGGAGTTGGTAGATGTCCTTCAGATAACGGATAAGCAGCTTATGGAGATTAAAGCAGCTTCTTTACGAACATTAACATCAATTGTCCACTTGGAGAGAACTCCCAAACTCAGCAGTATTATTGACTGTACTGGAACTGCCTCCTACCATGGATTTTTGCCAGTGCTTGTAAGGAACTGTATCCAGGCCATGATTGATCCTTCCATGGATCCATACCCTCACCAGTTTGCCACTGCTCTCTTCTCTTTTTTATACCATCTGGCCAGCTACGATGCTGGTGGTGAAGCCTTGGTCTCCTGTGGAATGATGGAAGCCTTATTGAAGGTCATAAAGTTTCTTGGCGATGAACAGGACCAGATAACATTTGTCACCAGAGCCGTCAGAGTGGTTGACCTTATCACCAACCTGGATATGGCAGCTTTTCAATCCCATAGTGGACTTTCTATCTTCATTTATAGACTTGAGCATGAAGTAGATTTGTGCCGAAAAGAATGTCCGTTTGTGATCAAGCCAAAGATCCAGAGACCCAATACTACACAAGAAGGAGAGGAAATGGAAACTGATATGGATGGAGTCCAGTGTATTCCACAACGAGCAGTACTTCTGAAATCCATGTTGAATTTCCTCAAGAAGGCCATCCAAGACCCTACTTTCTCAGATGGCATACGACATGTGATGGATGGTTCTCTGCCTACCTCCCTGAAACACATCATCAGCAATGCAGAATACTATGGCCCATCACTCTTCCTCCTAGCTACTGAAGTGGTGACTGTGTTTGTATTTCAAGAACCATCACTGCTCTCCTCACTCCAGGACAATGGATTGACAGATGTCATGCTGCATGCACTGCTTATCAAAGATGTTCCTGCTACCCGTGAAGTCCTTGGCTCCCTCCCAAATGTATTCAGTGCACTCTGTTTGAATGCCCGAGGTCTTCAGTCTTTTGTTCAGTGTCAGCCTTTTGAACGCCTCTTCAAAGTTCTTCTGTCTCCAGATTACCTCCCAGCCATGCGGAGGAGGAGAAGTTCTGATCCCCTTGGGGATACTGCATCCAACCTGGGGAGTGCTGTCGATGAGCTCATGAGACATCAGCCCACCCTTAAAACAGATGCAACGACTGCCATCATCAAGTTACTTGAAGAAATCTGTAATCTTGGAAGGGACCCCAAATACATCTGTCAGAAGCCATCAATCCAGAAGGCAGATGGCACTGCCACTGCTCCTCCCCCAAGGTCTAATCATGCCGCAGAAGAAGCCTCTAGTGAGGATGAGGAGGAAGAGGAAGTACAGGCCATGCAGAGCTTTAATTCTACCCAGCAAAATGAAACTGAGCCTAATCAGCAGGTTGTTGGTACAGAGGAACGTATTCCTATTCCCCTCATGGATTACATCCTTAATGTGATGAAATTTGCGGAATCTATTCTGAGCAACAATACAACAGATGACCACTGCCAGGAATTTGTGAATCAGAAAGGACTGTTGCCTTTGGTTACCATTTTGGGTCTTCCCAATCTGCCCATTGACTTTCCCACATCTGCTGCCTGTCAGGCTGTTGCAGGTGTCTGCAAATCCATATTGACACTGTCACATGAACCCAAAGTCCTTCAAGAGGGTCTCCTTCAGTTGGACTCCATCCTCTCCTCCCTGGAGCCCTTACACCGCCCCATTGAATCCCCTGGGGGCTCAGTGTTGTTGCGAGAACTGGCTTGCGCAGGCAATGTTGCTGGTGCTACCCTCTCAGCCCAGGCCACACCTCTGCTGCATGCACTCACTGCTGCCCATGCCTACATCATGATGTTTGTTCATACTTGCAGAGTTGGACAGAGTGAAATTCGTTCCATCTCCGTAAACCAGTGGGGCTCTCAATTGGGTCTGAGTGTTTTGAGCAAGCTGAGCCAGTTATACTGTTCCCTGGTGTGGGAAAGCACTGTCCTCCTCTCTCTGTGTACCCCAAACAGCCTACCATCTGGGTGTGAATTTGGCCAGGCAGATATGCAGAAACTGGTTCCAAAGGATGAGAAGGCAGGTACGACCCAGGGTGGAAAAAGATCAGATGGGGAACAGGATGGAGCAGCTGGAAGTTAATCGCGA

Amino Acid Sequence

MYPYDVPDYAGSMKVDRTKLKKTPTEAPADCRALIDKLKVCNDEQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVENMSWMLVCDRPEREQ
LKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHM
MKYPPSATTLHFEFYADPGAEVKIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMYSIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILV
YSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTPKLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMDPYPHQFATALFS
FLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNTTQEG
EEMETDMDGVQCIPQRAVLLKSMLNFLKKAIQDPTFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHA
LLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEI
CNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFAESILSNNTTDD
HCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVAGATLSAQ
ATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGGK
RSDGEQDGAAGS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU50837
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Changes from NM_031407.6 CDS:; ; C506V; A523T; V777A; D882G