cDNA Clone Erk5

• Plasmid: pCAGGS puro FLAG mouse ERK5 D200A
• Parent Plasmid: pCAGGS puro
• Expressed: FLAG mouse ERK5 D200A
• Genbank: AB019373.1 GI:5360520 with silent changes - see notes
• Tag: FLAG
• Species: Mouse

Sequence of Insert

CTCGAGCCACCATGGACTACAAGGACGACGATGACAAGGCCGAACCGCTGAAGGAGGAGGACGGCGAAGATGGCTCTGGGGAGCCCCCTGGGAGGGTGAAGGCAGAACCCGTTCACACCGCTGCCTCTGTGGTGGCCAAGAACCTGGCCCTGCTCAAAGCCCGCTCCTTCGACGTGACCTTTGACGTGGGGGACGAGTACGAGATCATCGAGACCATAGGCAATGGGGCCTACGGGGTGGTGTCTTCGGCGCGCCGCCGCCTCACGGGCCAGCAGGTGGCCATCAAGAAGATACCTAATGCTTTTGATGTGGTGACCAATGCCAAACGGACCCTCAGGGAGCTGAAGATCCTCAAACACTTCAAACACGACAATATCATCGCCATCAAGGACATCCTGAAGCCTACTGTGCCCTATGGAGAATTCAGATCTGTCTATGTGGTACTGGACCTCATGGAGAGCGACCTACACCAGATCATTCACTCTTCACAGCCGCTCACCCTGGAACATGTGAGATACTTCCTGTACCAGCTGCTTCGGGGCCTCAAATACATGCACTCTGCTCAGGTCATCCACCGTGATCTTAAACCCTCTAACCTTCTGGTCAATGAGAACTGTGAGCTCAAGATCGGTGCCTTTGGAATGGCCCGTGGTCTCTGTACTTCCCCTGCCGAGCACCAGTACTTCATGACTGAGTATGTGGCTACTCGCTGGTACCGTGCCCCGGAGCTCATGCTTTCCCTGCACGAGTATACGCAGGCAATCGACCTCTGGTCTGTGGGCTGCATCTTTGGTGAGATGCTGGCTCGGCGCCAGCTCTTCCCAGGCAAAAACTACGTGCACCAGTTACAGCTGATCATGATGGTGTTGGGAACTCCGTCACCAGCTGTGATTCAGGCTGTGGGGGCTGAAAGGGTGCGAGCCTATATCCAGAGCCTGCCACCAAGGCAACCTGTGCCTTGGGAGACAGTATACCCAGGTGCTGACCGCCAGGCCCTCTCCCTGCTGGGACGCATGTTGCGATTTGAACCCAGTGCCCGAATCTCAGCTGCTGCTGCCCTTCGCCACCCCTTCCTGGCTAAGTACCATGACCCTGATGATGAGCCTGATTGCGCCCCACCTTTTGACTTTGCTTTTGACCGTGAAGCCCTTACCAGGGAGCGCATTAAGGAGGCCATTGTGGCTGAGATTGAGGACTTCCACGCACGACGGGAGGGCATCCGCCAACAAATCCGCTTCCAGCCTTCTCTGCAGCCTGTGGCTAGTGAGCCTGTGTGTCCAGATGTTGAGATGCCCAGTCCCTGGGCTCCAAGTGGAGACTGTGCCATGGAGTCGCCTCCTCCAGCACTGCCACCATGCTCTGATCCTGCACCTGACACCGTTGATCTGACTCTGCAGCCTGCCCCGCCGGCCAGTGAGCTTGCTCCACCAAAAAGAGAGGGTGCCATCTCCGACAATACCAAAGCAGCCCTCAAAGCTGCCCTGCTCAAGTCCCTAAGGAGCAGGCTCAGAGATGGGCCCAGTGCACCCTTGGAGGCGCCTGAGCCTCGAAAGCCCGTGACAGCTCAGGAACGCCAGCGGGAACGAGAAGAGAAGCGCAGGAGGCGACAAGAGAGAGCCAAGGAGCGGGAGAAGCGACGACAAGAGAGAGAACGCAAGGAGAGGGGGGCTGGTACCTTGGGGGGCCCCTCTACTGACCCTCTGGCTGGACTGGTGCTCAGTGACAATGACCGAAGCCTGCTAGAGCGGTGGACTCGCATGGCTAGGCCTCCTGCCCCTGCCCCTGCCCCAGCGCCAGCACCAGCGCCAGCACCGTCCTCTGCCCAGCCCACTAGTACTCCTACTGGCCCCGTATCTCAGTCTACTGGTCCTCTACAGCCTGCAGGCTCTATTCCGGGTCCTGCCTCCCAGCCTGTTTGCCCACCCCCTGGCCCTGTTCCCCAGCCTGCTGGCCCTATCCCTGCTCCGCTCCAGACTGCCCCTTCCACTAGCCTTTTGGCCTCCCAGTCACTTGTGCCACCTAGTGGGTTGCCTGGTTCTGGTGCCCCAGAAGTTCTGCCTTACTTCCCATCTGGCCCACCACCTCCAGATCCTGGGCTCACCCCTCAGCCTTCTACATCAGAGTCACCTGATGTCAACCTGGTGACTCAGCAGCTGTCCAAGTCTCAGGTGGAGGACCCCCTGCCTCCTGTGTTCTCTGGCACTCCAAAGGGCAGTGGGGCTGGCTACGGAGTTGGCTTTGATCTGGAGGAATTCTTAAATCAATCTTTTGATATGGGTGTGGCTGATGGGCCACAGGATGGCCAGGCAGACTCAGCCTCACTCTCAGCCTCTCTCCTTGCTGACTGGCTTGAGGGCCATGGCATGAACCCTGCTGACATTGAGTCTCTGCAGCGTGAGATCCAGATGGACTCCCCAATGCTGCTGTCTGACCTGCCTGACCTCCAAGAGCCCTGACTCGAG

Amino Acid Sequence

MDYKDDDDKAEPLKEEDGEDGSGEPPGRVKAEPVHTAASVVAKNLALLKARSFDVTFDVGDEYEIIETIGNGAYGVVSSARRRLTGQQVAIKKIPNAFDV
VTNAKRTLRELKILKHFKHDNIIAIKDILKPTVPYGEFRSVYVVLDLMESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVNE
NCELKIGAFGMARGLCTSPAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGTPSPAVIQAVGAE
RVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAAALRHPFLAKYHDPDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHARR
EGIRQQIRFQPSLQPVASEPVCPDVEMPSPWAPSGDCAMESPPPALPPCSDPAPDTVDLTLQPAPPASELAPPKREGAISDNTKAALKAALLKSLRSRLR
DGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKEREKRRQERERKERGAGTLGGPSTDPLAGLVLSDNDRSLLERWTRMARPPAPAPAPAPAPAPA
PSSAQPTSTPTGPVSQSTGPLQPAGSIPGPASQPVCPPPGPVPQPAGPIPAPLQTAPSTSLLASQSLVPPSGLPGSGAPEVLPYFPSGPPPPDPGLTPQP
STSESPDVNLVTQQLSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDGQADSASLSASLLADWLEGHGMNPADIESLQREIQMD
SPMLLSDLPDLQEP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU50862
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent changes: c618t, t1167c, a1545g