cDNA Clone BRSK2

• Plasmid: pcDNA5 FRT/TO FLAG-HA-BRSK2 D159A
• Parent Plasmid: pcDNA5 FRT/TO FLAG
• Expressed: FLAG-HA-BRSK2 KD
• Genbank: NM_001256629
• Tag: HA
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCACATCGACGGGGAAGGACGGCGGCGCGCAGC
ACGCGCAGTATGTTGGGCCCTACCGGCTGGAGAAGACGCTGGGCAAGGGGCAGACAGGTCTGGTGAAGCTGGGGGTTCACTGCGTCACCTGCCAGAAGGT
GGCCATCAAGATCGTCAACCGTGAGAAGCTCAGCGAGTCGGTGCTGATGAAGGTGGAGCGGGAGATCGCGATCCTGAAGCTCATTGAGCACCCCCACGTC
CTAAAGCTGCACGACGTTTATGAAAACAAAAAATATTTGTACCTGGTGCTAGAACACGTGTCAGGTGGTGAGCTCTTCGACTACCTGGTGAAGAAGGGGA
GGCTGACGCCTAAGGAGGCTCGGAAGTTCTTCCGGCAGATCATCTCTGCGCTGGACTTCTGCCACAGCCACTCCATATGCCACAGGGATCTGAAACCTGA
AAACCTCCTGCTGGACGAGAAGAACAACATCCGCATCGCAGcCTTTGGCATGGCGTCCCTGCAGGTTGGCGACAGCCTGTTGGAGACCAGCTGTGGGTCC
CCCCACTACGCCTGCCCCGAGGTGATCCGGGGGGAGAAGTATGACGGCCGGAAGGCGGACGTGTGGAGCTGCGGCGTCATCCTGTTCGCCTTGCTGGTGG
GGGCTCTGCCCTTCGACGATGACAACTTGCGACAGCTGCTGGAGAAGGTGAAGCGGGGCGTGTTCCACATGCCGCACTTTATCCCGCCCGACTGCCAGAG
TCTGCTACGGGGCATGATCGAGGTGGACGCCGCACGCCGCCTCACGCTAGAGCACATTCAGAAACACATATGGTATATAGGGGGCAAGAATGAGCCCGAA
CCAGAGCAGCCGATTCCTCGCAAGGTGCAGATCCGCTCGCTGCCCAGCCTGGAGGACATCGACCCCGACGTGCTGGACAGCATGCACTCACTGGGCTGCT
TCCGAGACCGCAACAAGCTGCTGCAGGACCTGCTGTCCGAGGAGGAGAACCAGGAGAAGATGATTTACTTCCTCCTCCTGGACCGGAAAGAAAGGTACCC
GAGCCAGGAGGATGAGGACCTGCCCCCCCGGAACGAGATAGACCCTCCCCGGAAGCGTGTGGACTCCCCGATGCTGAACCGGCACGGCAAGCGGCGGCCA
GAACGCAAATCCATGGAGGTGCTCAGCGTGACGGACGGCGGCTCCCCGGTGCCTGCGCGGCGGGCCATTGAGATGGCCCAGCACGGCCAGAGGTCTCGGT
CCATCAGCGGTGCCTCCTCAGGCCTTTCCACCAGCCCACTCAGCAGCCCCCGGGTGACCCCTCACCCCTCACCAAGGGGCAGTCCCCTCCCCACCCCCAA
GGGGACACCTGTCCACACGCCAAAGGAGAGCCCGGCTGGCACGCCCAACCCCACGCCCCCGTCCAGCCCCAGCGTCGGAGGGGTGCCCTGGAGGGCGCGG
CTCAACTCCATCAAGAACAGCTTTCTGGGCTCACCCCGCTTCCACCGCCGGAAACTGCAAGTTCCGACGCCGGAGGAGATGTCCAACCTGACACCAGAGT
CGTCCCCAGAGCTGGCGAAGAAGTCCTGGTTTGGGAACTTCATCAGCCTGGAGAAGGAGGAGCAGATCTTCGTGGTCATCAAAGACAAACCTCTGAGCTC
CATCAAGGCTGACATCGTGCACGCCTTCCTGTCGATTCCCAGTCTCAGCCACAGCGTCATCTCCCAAACGAGCTTCCGGGCCGAGTACAAGGCCACGGGG
GGGCCAGCCGTGTTCCAGAAGCCGGTCAAGTTCCAGGTTGATATCACCTACACGGAGGGTGGGGAGGCGCAGAAGGAGAACGGCATCTACTCCGTCACCT
TCACCCTGCTCTCAGGCCCCAGCCGTCGCTTCAAGAGGGTGGTGGAGACCATCCAGGCCCAGCTGCTGAGCACACACGACCCGCCTGCGGCCCAGCACTT
GTCAGAACCCCCCCCACCAGCGCCAGGACTAAGCTGGGGTGCTGGGCTTAAGGGCCAGAAGGTGGCCACCAGCTACGAGAGTAGCCTCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSATMYPYDVPDYATSTGKDGGAQHAQYVGPYRLEKTLGKGQTGLVKLGVHCVTCQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHV
LKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDEKNNIRIAAFGMASLQVGDSLLETSCGS
PHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKNEPE
PEQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDRKERYPSQEDEDLPPRNEIDPPRKRVDSPMLNRHGKRRP
ERKSMEVLSVTDGGSPVPARRAIEMAQHGQRSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSSPSVGGVPWRAR
LNSIKNSFLGSPRFHRRKLQVPTPEEMSNLTPESSPELAKKSWFGNFISLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKATG
GPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFKRVVETIQAQLLSTHDPPAAQHLSEPPPPAPGLSWGAGLKGQKVATSYESSL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28124
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41457. Transcript variant 3