cDNA Clone DCUN1D3

• Plasmid: pSc-B-DCUN1D5 (Met1-Phe46)-DCUN1D3 (Ser86-Thr304)
• Parent Plasmid: pSC-B
• Species: Human

Sequence of Insert

GGATCCATGCCGGTGAAGAAGAAGAGAAAATCCCCTGGGGTGGCAGCAGCAGTAGCGGAAGACGGAGGCCTCAAAAAGTGTAAAATCTCCAGCTATTGCAGATCCCAACCCCCTGCTAGACTAATAAGTGGAGAGGAACATTTTTCTTCCTTGCAAAGATTGGAAGAACTGTTCAGGCGCTACAAGGATGAGCGGGAAGATGCAATTTTGGAGGAAGGCATGGAGCGCTTTTGCAATGACCTGTGTGTTGACCCCACAGAATTTCGAGTGCTGCTCTTGGCTTGGAAGTTCCAGGCTGCAACCATGTGCAAATTCACCAGGAAGGAGTTTTTTGATGGCTGCAAAGCAATAAGTGCAGACAGCATTGACGGAATCTGTGCACGGTTCCCTAGCCTCTTAACAGAAGCCAAACAAGAGGATAAATTCAAGGATCTCTACCGGTTTACATTTCAGTTTGGCCTGGACTCTGAAGAAGGGCAGCGGTCACTGCATCGGGAAATAGCCATTGCCCTGTGGAAACTAGTCTTTACCCAGAACAATCCTCCGGTATTGGACCAATGGCTAAACTTCCTAACAGAGAACCCCTCGGGGATCAAGGGCATCTCCCGGGACACTTGGAACATGTTCCTTAACTTCACTCAGGTGATTGGCCCTGACCTCAGCAACTACAGTGAAGATGAGGCCTGGCCAAGTCTCTTTGACACCTTTGTGGAGTGGGAAATGGAGCGAAGGAAAAGAGAAGGGGAAGGGAGAGGTGCACTCAGCTCAGGGCCTGAGGGCTTGTGTCCCGAGGAGCAGACTTAGGCGGCCGC

Amino Acid Sequence

MPVKKKRKSPGVAAAVAEDGGLKKCKISSYCRSQPPARLISGEEHFSSLQRLEELFRRYKDEREDAILEEGMERFCNDLCVDPTEFRVLLLAWKFQAATM
CKFTRKEFFDGCKAISADSIDGICARFPSLLTEAKQEDKFKDLYRFTFQFGLDSEEGQRSLHREIAIALWKLVFTQNNPPVLDQWLNFLTENPSGIKGIS
RDTWNMFLNFTQVIGPDLSNYSEDEAWPSLFDTFVEWEMERRKREGEGRGALSSGPEGLCPEEQT*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU28134
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Chimaeric fusion comprising amino acids 1-46 of the N-terminus of DCUN1D5 onto the PONY domain (aa 86-304) of DCUN1D3. Constructed by two successive rounds of PCR amplifying DCUN1D3 PONY domain from 24948, sequentially adding on D5 sequence at the N-terminus