cDNA Clone Mint1

• Plasmid: pEBG Mint1
• Parent Plasmid: pEBG6P
• Expressed: GST-Mint1
• Genbank: NM_001163.3
• Tag: GST
• Species: Human

Sequence of Insert

TCTGCGGAGGTGGAGGTGACCGACGAGGCGGCAGGTGGGGAGGTGAACGAGTCGGTGGAGGCCGACCTGGAGCACCCCGAGGTGGAAGAGGAACAGCAGCAGCCGCCGCAGCAGCAGCACTATGTGGGCCGCCACCAGCGCGGGCGAGCCCTCGAGGACCTCCGCGCCCAGCTCGGCCAGGAGGAAGAGGAGCGCGGGGAATGCCTGGCGCGCTCAGCCAGCACGGAGAGCGGCTTCCACAACCACACAGACACCGCCGAGGGCGACGTGATCGCCGCGGCCCGCGACGGCTACGATGCGGAGCGCGCGCAGGACCCCGAGGACGAGAGCGCCTATGCTGTGCAGTACCGGCCCGAGGCCGAGGAGTACACGGAGCAGGCAGAGGCCGAGCACGCCGAGGCCACGCACCGCCGCGCGCTGCCCAACCACCTGCACTTCCACTCGCTGGAGCACGAGGAAGCCATGAATGCGGCCTACTCAGGCTACGTCTACACGCACCGGCTCTTCCACCGCGGTGAGGACGAGCCCTACTCCGAGCCCTATGCCGACTACGGCGGCCTCCAGGAGCACGTGTACGAGGAGATAGGGGACGCGCCCGAGCTGGACGCACGCGACGGCCTGCGGCTCTACGAGCAGGAGCGCGACGAGGCGGCCGCGTACCGCCAGGAGGCCCTGGGCGCGCGGCTGCACCATTACGACGAGCGCTCCGACGGCGAGTCCGACAGCCCCGAGAAGGAGGCCGAGTTCGCGCCCTACCCGCGCATGGACAGCTACGAGCAGGAGGAGGACATCGACCAGATAGTGGCCGAGGTGAAGCAGAGCATGAGCTCGCAGAGCCTCGACAAGGCAGCCGAGGACATGCCTGAGGCCGAGCAGGACCTGGAGCGTCCCCCTACCCCGGCCGGGGGTCGCCCCGACAGCCCCGGGCTGCAGGCGCCGGCGGGGCAGCAGCGGGCGGTGGGCCCCGCGGGCGGCGGCGAGGCGGGGCAGCGGTACAGCAAGGAGAAGCGCGATGCCATCTCGCTGGCCATCAAGGACATCAAGGAGGCCATCGAGGAGGTGAAAACCAGGACCATCCGTTCGCCTTACACCCCCGACGAGCCCAAAGAGCCCATCTGGGTCATGCGCCAGGACATTAGCCCCACCAGGGACTGTGACGACCAGAGGCCGATGGACGGAGATTCTCCGTCTCCTGGCAGCTCCTCCCCCTTGGGTGCAGAGTCATCAAGCACATCTCTTCACCCCAGTGACCCTGTGGAAGCGTCCACTAATAAAGAGTCAAGAAAAAGCTTGGCTTCATTCCCAACCTACGTTGAAGTTCCGGGACCCTGCGACCCCGAAGACTTGATCGATGGAATCATTTTTGCCGCCAATTACCTTGGCTCCACTCAGCTGCTCTCAGACAAAACTCCTTCCAAAAACGTGCGCATGATGCAGGCCCAGGAAGCCGTAAGCAGGATCAAGATGGCCCAGAAATTAGCCAAAAGCAGGAAGAAGGCTCCTGAAGGCGAATCTCAGCCAATGACTGAAGTGGATCTCTTCATTTCTACCCAGAGAATCAAAGTGCTGAACGCCGACACACAGGAGACAATGATGGACCACCCTCTGAGGACCATTTCCTACATTGCGGACATTGGGAACATCGTTGTGCTGATGGCCCGCCGGCGGATGCCTCGCTCCAACTCCCAGGAGAACGTGGAAGCGTCCCACCCATCCCAGGATGGGAAAAGGCAGTACAAGATGATCTGCCACGTCTTCGAGTCTGAGGATGCTCAGCTGATTGCACAGCCCATCGGACAGGCATTTAGCGTGGCATACCAGGAATTCCTCAGGGCCAATGGGATTAACCCCGAAGATCTCAGCCAGAAGGAGTATAGTGACCTGCTCAATACCCAGGACATGTACAACGATGACCTGATCCACTTCTCCAAGTCGGAAAACTGTAAAGATGTTTTCATAGAGAAGCAGAAAGGAGAAATCCTAGGTGTGGTGATTGTGGAGTCTGGCTGGGGATCCATCCTCCCCACCGTGATCATTGCCAACATGATGCATGGTGGCCCTGCGGAGAAATCTGGGAAGCTGAATATCGGTGACCAGATCATGTCCATTAATGGCACCAGCCTGGTGGGCCTGCCTCTGTCCACCTGCCAGAGCATTATTAAGGGCTTAAAGAATCAGTCCCGAGTCAAGCTGAATATCGTGAGATGTCCTCCGGTGACCACCGTGTTAATCAGAAGACCAGACCTTCGCTACCAGCTCGGTTTCAGCGTCCAGAATGGAATTATCTGCAGCCTCATGCGAGGGGGAATAGCTGAGAGAGGAGGCGTCCGTGTGGGGCACCGGATCATTGAAATCAATGGACAGAGCGTCGTGGCCACCCCCCACGAGAAGATCGTCCACATTCTCTCCAATGCTGTTGGGGAGATTCATATGAAGACAATGCCAGCCGCGATGTACAGGCTGCTGACGGCCCAGGAGCAGCCTGTTTACATCTGACCGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSRIRSAEVEVTDEAAGGEVNESVEADLEHPEVEEEQQQPPQQQHYVGRHQRGRALEDLRAQLGQEEEERGECLARSASTESGFHNHTDTAEGDVIAAARDGYDAERAQDPEDESAYAVQYRPEAEEYTEQAEAEHAEATHRRALPNHLHFHSLEHEEAMNAAYSGYVYTHRLFHRGEDEPYSEPYADYGGLQEHVYEEIGDAPELDARDGLRLYEQERDEAAAYRQEALGARLHHYDERSDGESDSPEKEAEFAPYPRMDSYEQEEDIDQIVAEVKQSMSSQSLDKAAEDMPEAEQDLERPPTPAGGRPDSPGLQAPAGQQRAVGPAGGGEAGQRYSKEKRDAISLAIKDIKEAIEEVKTRTIRSPYTPDEPKEPIWVMRQDISPTRDCDDQRPMDGDSPSPGSSSPLGAESSSTSLHPSDPVEASTNKESRKSLASFPTYVEVPGPCDPEDLIDGIIFAANYLGSTQLLSDKTPSKNVRMMQAQEAVSRIKMAQKLAKSRKKAPEGESQPMTEVDLFISTQRIKVLNADTQETMMDHPLRTISYIADIGNIVVLMARRRMPRSNSQENVEASHPSQDGKRQYKMICHVFESEDAQLIAQPIGQAFSVAYQEFLRANGINPEDLSQKEYSDLLNTQDMYNDDLIHFSKSENCKDVFIEKQKGEILGVVIVESGWGSILPTVIIANMMHGGPAEKSGKLNIGDQIMSINGTSLVGLPLSTCQSIIKGLKNQSRVKLNIVRCPPVTTVLIRRPDLRYQLGFSVQNGIICSLMRGGIAERGGVRVGHRIIEINGQSVVATPHEKIVHILSNAVGEIHMKTMPAAMYRLLTAQEQPVYI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU26295
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: THis clone starts at aa7 (SAE) of the reference sequence stated. This clone has 1 non silent mutation as compared with the reference sequence NM_001163.3. S603P repeated attempts to correct this mutation have yielded nothing. ALso has a silent mutation at T89. 3' NOT1 site not usable due to there being an internal NOt1 site.