cDNA Clone Sc TOF1 (1 - 222)

• Plasmid: pGEX6P1 Sc Tof1 1-222
• Parent Plasmid: pGEX 6P-1
• Expressed: GST- Sc TOF1 (1 - 222)
• Tag: GST
• Species: Saccharomyces cerevisiae

Sequence of Insert

GAATTCGCCCTTGCTAGCATGTCTGCTGATTTGCAACAAGGCACTACAAATGCGGCTGATTTCTCTCTGACGGTACTTAGAGCAAGAATTGCTTTATTGGCAACTGCCATCGGTGGGCCGGATTATACTTCCCAGATAGACCCACCCCCTTATAAGCTTGGCGATGACTGTTTGGCGTGCTTAAAAGATCTGAAAAGATGGTTTAAACTAGTGGATGACCAGCAGAAAAGATGGGATGTGGCAATGGCAGTAGCTGAATATCGCATCTTGACGGATGATCTGTTACCTATTCTGATAGATTGGGAAAACAAATGTTCTCTTGCAGCAAAGCTTGCAAAGAACAACCCAGACCATGAAGAGTTTAGAAACAAAGCTTATTACGATAAAATTGCGCTAAATTGTCTACAACTATTGGTCCTTATGACTTGGCCTTTAATTGTAACTGAACAGTCTTCATCAAATCAAATTACTCTCTATGGTGAGCTAAAGAAGCATCAATTGGTATACAAGAAAACGATTCTGTCTATGGAAAGTGGTAAAGTGTTAAGAGCTGCCATTAGATTGGCTCTGGATGTTATCAAAATCGACCGATTATCAAGAACTCCTAGGGACAATATGGTTCTCAAATTGGTTTTGAATTTTTTCAGAAATGTCATTGCTATAGAACCCGGTGAATTTACTATCTAACTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFALASMSADLQQGTTNAADFSLTVLRARIALLATAIGGPDYTSQIDPPPYKLGDDCLACLKDLKRWFKLVDDQQKRWDVAMAVAEYRILTDDLLPILIDWENKCSLAAKLAKNNPDHEEFRNKAYYDKIALNCLQLLVLMTWPLIVTEQSSSNQITLYGELKKHQLVYKKTILSMESGKVLRAAIRLALDVIKIDRLSRTPRDNMVLKLVLNFFRNVIAIEPGEFTI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU47907
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Already have His6 tagged version [DU 47649]