cDNA Clone FAM83H

• Plasmid: pCS2+ GFP FAM83H F270A F274A
• Parent Plasmid: pCS2+ GFP
• Expressed: GFP FAM83H F270/274A
• Genbank: NM_198488.3
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCCCGTCGCTCTCAGAGCTCCTCGCAGGGGGACAACCCACT
GGCACCCGGGTACCTGCCGCCTCACTACAAAGAGTACTACCGCCTGGCGG
TGGATGCACTGGCCGAGGGTGGCTCGGAGGCCTACAGCCGCTTCCTCGCT
ACCGAGGGGGCACCAGACTTCCTGTGCCCTGAAGAGCTGGAACATGTGAG
CCGACACCTTCGGCCTCCGCAGTATGTTACCCGAGAGCCACCTGAAGGCA
GCCTTCTCGACGTGGACATGGATGGCTCCTCGGGTACATACTGGCCAGTG
AACTCAGACCAGGCCGTGCCTGAGCTTGATTTGGGCTGGCCTCTGACCTT
CGGCTTCCAGGGCACCGAGGTGACCACCTTGGTGCAGCCACCGCCCCCCG
ACAGCCCCAGTATCAAGGATGAGGCCCGCAGGATGATCCGTTCCGCCCAG
CAGGTGGTGGCCGTGGTGATGGACATGTTCACTGATGTGGACCTGCTCAG
CGAAGTGCTGGAGGCCGCGGCCCGTCGGGTCCCAGTCTACATCCTGCTGG
ATGAGATGAACGCGCAGCACTTCCTGGACATGGCCGACAAGTGCCGTGTC
AACCTGCACCACGTGGATTTCCTGCGCGTACGGACTGTGGCGGGCCCCAC
CTACTACTGCCGCACTGGGAAGTCCTTCAAGGGCCACGTCAAGGAGAAGT
TCCTGCTGGTGGACTGTGCCGTGGTGATGAGTGGGAGCTACAGCTTCATG
TGGTCCTTTGAGAAGATCCACCGCAGCCTGGCGCACGTGTTCCAAGGAGA
GCTGGTCTCCAGCGCCGACGAGGAGGCCCGCATCCTCTTCGCGCAGTCCG
AGCCGCTTGTGCCCTCGGCCGCGGCCCTGGCCCGCATGGACGCCTATGCC
CTGGCTCCGTATGCCGGGGCCGGGCCTCTCGTGGGCGTCCCTGGGGTCGG
GGCGCCAACCCCCTTCTCCTTCCCTAAACGAGCGCACCTCCTGTTCCCGC
CACCCCGGGAAGAGGGCCTGGGCTTCCCCTCCTTCCTCGACCCGGACCGC
CACTTCCTGTCGGCCTTCCGCCGGGAGGAGCCGCCGCGGATGCCGGGGGG
CGCGCTGGAACCGCACGCGGGGCTGCGGCCACTCTCGCGGCGCCTGGAGG
CCGAGGCCGGGCCGGCTGGGGAGCTCGCGGGCGCGCGGGGCTTCTTCCAG
GCGCGGCACCTGGAGATGGACGCCTTCAAGCGGCACAGCTTCGCGACCGA
GGGCGCGGGCGCCGTGGAGAACTTCGCGGCTGCGCGGCAGGTGTCGCGGC
AGACGTTCCTCAGCCACGGCGACGACTTCCGCTTCCAGACCAGCCACTTC
CACCGTGACCAGCTCTACCAGCAGCAGTACCAGTGGGACCCGCAGCTCAC
GCCGGCGCGCCCGCAAGGCCTGTTCGAGAAGCTTCGCGGGGGCCGCGCGG
GTTTCGCGGACCCGGATGACTTCACCCTGGGCGCCGGGCCCCGCTTCCCG
GAGCTCGGACCCGACGGGCACCAGCGGCTGGACTACGTGCCGTCCAGCGC
GTCCCGCGAGGTGCGCCACGGCTCGGACCCCGCCTTCGCGCCCGGACCCC
GCGGCCTGGAGCCCAGCGGAGCCCCGCGCCCCAACCTGACCCAGCGCTTC
CCATGCCAGGCCGCGGCGAGGCCGGGCCCAGACCCCGCTCCCGAGGCGGA
GCCGGAGCGCAGGGGCGGGCCCGAGGGGCGGGCAGGGCTGCGGCGCTGGC
GTTTGGCCTCCTACTTGAGCGGCTGCCACGGCGAGGATGGGGGCGACGAC
GGCCTACCGGCGCCCATGGAAGCGGAGGCTTACGAAGACGACGTGCTGGC
TCCCGGGGGCCGGGCACCTGCCGGCGACCTGCTCCCCTCGGCCTTCCGCG
TCCCAGCAGCCTTCCCCACCAAGGTCCCGGTGCCAGGCCCGGGCAGCGGC
GGCAACGGCCCAGAGCGCGAGGGCCCGGAGGAGCCTGGCCTGGCCAAGCA
GGACTCATTCCGCTCGCGCCTGAACCCCCTGGTCCAGCGCAGCTCCAGGC
TGCGCTCCTCGCTCATCTTCAGCACGTCACAGGCCGAGGGCGCGGCCGGG
GCTGCGGCGGCCACTGAGAAGGTGCAGCTGCTGCACAAGGAGCAGACGGT
CAGCGAGACGCTGGGGCCCGGCGGAGAGGCCGTGCGCTCCGCGGCTTCCA
CCAAGGTGGCGGAGCTGCTGGAGAAGTACAAGGGCCCAGCCCGTGATCCC
GGCGGCGGCGCGGGCGCCATCACCGTTGCCAGCCACAGCAAGGCCGTCGT
GTCCCAGGCGTGGCGGGAAGAGGTGGCGGCCCCAGGTGCCGTGGGGGGCG
AGCGCCGCAGCCTCGAGAGCTGCCTGCTGGACCTGCGCGACTCCTTTGCA
CAGCAGCTGCACCAGGAGGCGGAGCGGCAGCCGGGAGCCGCGTCGCTCAC
CGCGGCGCAGCTGCTCGACACACTGGGCCGGAGCGGCTCCGACCGCCTGC
CTTCCCGCTTCCTCTCTGCCCAGAGCCACTCAACGTCCCCGCAAGGGCTG
GACAGCCCTCTGCCGCTGGAAGGGTCCGGAGCGCACCAGGTGCTCCATAA
TGAGTCAAAAGGGAGCCCCACCTCGGCTTACCCTGAGCGGAAGGGGAGCC
CCACGCCTGGGTTTTCCACTCGAAGAGGAAGTCCAACTACAGGATTTATC
GAGCAGAAGGGGAGCCCCACCTCAGCCTACCCCGAGCGCAGGGGTAGTCC
GGTGCCCCCCGTGCCGGAGCGCAGGAGCAGTCCGGTGCCCCCCGTGCCGG
AGCGCAGGGGCAGCCTCACCCTTACCATCTCCGGGGAGTCCCCGAAGGCC
GGGCCCGCGGAGGAGGGGCCGAGCGGCCCCATGGAAGTCCTGCGCAAAGG
CTCCTTGCGTCTTAGGCAGCTGCTGAGCCCCAAGGGCGAGCGGCGCATGG
AGGATGAGGGTGGCTTCCCAGTGCCGCAGGAGAACGGCCAACCCGAGAGC
CCGCGGCGTCTGTCACTGGGCCAGGGTGACAGCACGGAGGCTGCCACAGA
AGAGCGGGGTCCGCGGGCGCGCCTGTCCTCAGCCACGGCCAACGCCTTGT
ACAGCAGCAACCTTCGGGATGACACGAAGGCCATTCTGGAGCAGATCAGT
GCCCACGGCCAGAAGCACCGTGCGGTCCCTGCCCCGAGCCCCGGCCCGAC
CCACAACAGCCCCGAGCTAGGCCGTCCACCGGCTGCTGGCGTCCTGGCCC
CAGATATGTCCGACAAGGACAAGTGTTCAGCCATCTTCCGCTCGGACAGC
TTGGGGACCCAGGGCCGGCTGAGCCGCACGCTGCCAGCCAGCGCGGAGGA
GCGCGATCGGCTGCTGCGCCGCATGGAGAGCATGCGCAAGGAGAAGCGCG
TGTACAGCCGCTTCGAGGTCTTCTGCAAGAAAGAGGAGGCCAGCAGCCCT
GGGGCAGGGGAAGGCCCCGCGGAGGAGGGCACCAGGGACAGCAAGGTGGG
CAAGTTCGTGCCCAAGATCCTGGGCACGTTCAAAAGCAAGAAGTGAGCGG
CCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSMARRSQSSSQGDNPLAPGYLPPHYKEYYRLAVDALAEGGSEAYSRFLATEGAPDFLCPE
ELEHVSRHLRPPQYVTREPPEGSLLDVDMDGSSGTYWPVNSDQAVPELDLGWPLTFGFQGTEVTTLVQPPPPDSPSIKDEARRMIRSAQQVVAVVMDMFT
DVDLLSEVLEAAARRVPVYILLDEMNAQHFLDMADKCRVNLHHVDFLRVRTVAGPTYYCRTGKSFKGHVKEKFLLVDCAVVMSGSYSFMWSFEKIHRSLA
HVFQGELVSSADEEARILFAQSEPLVPSAAALARMDAYALAPYAGAGPLVGVPGVGAPTPFSFPKRAHLLFPPPREEGLGFPSFLDPDRHFLSAFRREEP
PRMPGGALEPHAGLRPLSRRLEAEAGPAGELAGARGFFQARHLEMDAFKRHSFATEGAGAVENFAAARQVSRQTFLSHGDDFRFQTSHFHRDQLYQQQYQ
WDPQLTPARPQGLFEKLRGGRAGFADPDDFTLGAGPRFPELGPDGHQRLDYVPSSASREVRHGSDPAFAPGPRGLEPSGAPRPNLTQRFPCQAAARPGPD
PAPEAEPERRGGPEGRAGLRRWRLASYLSGCHGEDGGDDGLPAPMEAEAYEDDVLAPGGRAPAGDLLPSAFRVPAAFPTKVPVPGPGSGGNGPEREGPEE
PGLAKQDSFRSRLNPLVQRSSRLRSSLIFSTSQAEGAAGAAAATEKVQLLHKEQTVSETLGPGGEAVRSAASTKVAELLEKYKGPARDPGGGAGAITVAS
HSKAVVSQAWREEVAAPGAVGGERRSLESCLLDLRDSFAQQLHQEAERQPGAASLTAAQLLDTLGRSGSDRLPSRFLSAQSHSTSPQGLDSPLPLEGSGA
HQVLHNESKGSPTSAYPERKGSPTPGFSTRRGSPTTGFIEQKGSPTSAYPERRGSPVPPVPERRSSPVPPVPERRGSLTLTISGESPKAGPAEEGPSGPM
EVLRKGSLRLRQLLSPKGERRMEDEGGFPVPQENGQPESPRRLSLGQGDSTEAATEERGPRARLSSATANALYSSNLRDDTKAILEQISAHGQKHRAVPA
PSPGPTHNSPELGRPPAAGVLAPDMSDKDKCSAIFRSDSLGTQGRLSRTLPASAEERDRLLRRMESMRKEKRVYSRFEVFCKKEEASSPGAGEGPAEEGT
RDSKVGKFVPKILGTFKSKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28198
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent changes at Pro375 and Ala425 compared to NCBI ref. Parental 28161