cDNA Clone KIAA1390

• Plasmid: pGEX6P1 KIAA1390 A416G Y424A
• Parent Plasmid: pGEX6P1
• Expressed: GST-KIAA1390 A416G Y424A
• Tag: GST

Sequence of Insert

agatctATGGAATACCATCAGCCTGAGGATCCAGCCCCTGGTAAGGCCGGGACTGCAGAAGCAGTCATCCCTGAAAACCATGAGGTTCTGGCAGGCCCAGATGAGCACCCTCAGGACACAGATGCAAGAGATGCTGATGGGGAGGCTAGAGAACGGGAGCCAGCAGACCAAGCTTTGCTGCCTAGCCAGTGTGGGGACAACCTTGAGTCCCCTCTGCCTGAAGCTAGCTCAGCTCCACCGGGGCCAACCCTTGGGACACTGCCTGAAGTAGAGACAATAAGGGCATGCTCCATGCCCCAGGAGCTTCCTCAGTCCCCCAGGACCCGACAGCCTGAGCCAGATTTCTACTGTGTCAAGTGGATCCCTTGGAAAGGAGAACAGACACCCATCATCACCCAGAGCACTAACGGCCCTTGCCCTCTCCTTGCCATCATGAACATCCTCTTTCTTCAGTGGAAGGTGAAGCTCCCCCCGCAGAAGGAAGTGATCACATCGGATGAGCTCATGGCCCATCTTGGAAACTGCCTCCTGTCCATCAAGCCCCAGGAGAAGTCAGAGGGACTTCAGCTTAATTTTCAGCAGAATGTGGATGATGCAATGACAGTGCTGCCTAAACTGGCCACAGGTCTGGATGTCAATGTGCGATTCACAGGCGTCTCTGATTTTGAGTATACACCCGAGTGCAGTGTCTTTGACCTGCTAGGCATACCTCTGTACCATGGCTGGCTTGTTGATCCACAGAGTCCTGAGGCTGTGCGTGCAGTTGGGAAACTGAGTTACAACCAGCTGGTGGAGAGGATCATCACCTGCAAACACTCCAGTGACACCAACCTCGTGACAGAAGGCCTGATTGCAGAGCAGTTCCTGGAGACCACCGCGGCCCAGCTGACCTACCACGGACTGTGTGAGCTGACAGCAGCTGCTAAGGAGGGTGAACTTAGCGTCTTTTTCCGAAACAACCACTTTAGCACCATGACTAAGCATAAGAGTCACTTATACCTACTGGTCACTGACCAGGGCTTTCTACAGGAGGAGCAAGTCGTATGGGAGAGCCTGCACAATGTGGATGGAGACAGCTGCTTTTGTGACTCTGACTTTCACCTGAGTCATTCCCTGGGCAAGGGGCCTGGAGCAGAAGGTGGGAGTGGCTCCCCAGAAAAGCAGCTGCAGGTAGACCAGGACTACCTGATTGCTCTGTCCCTGCAGCAGCAACAGCCACGAGGCCCGCTGGGGCTTACCGACTTGGAGCTGGGCCAGCAGCTTCAGCAAGAGGAGGCTCAACAGCAGCAGGCAGCGCAGCCAGTGCGGATGCGGACGCGGGTCCTGTCACTGCAGGGGAGAGGAGCCACATCTGGACGCCCAGCCGGGGAGCGTCGGCAGAGGCCGAAGCACGAGTCAGACTGCATTCTGCTGTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEYHQPEDPAPGKAGTAEAVIPENHEVLAGPDEHPQDTDARDADGEAREREPADQALLPSQCGDNLESPLPEASSAPPGPTLGTLPEVETIRACSMPQELPQSPRTRQPEPDFYCVKWIPWKGEQTPIITQSTNGPCPLLAIMNILFLQWKVKLPPQKEVITSDELMAHLGNCLLSIKPQEKSEGLQLNFQQNVDDAMTVLPKLATGLDVNVRFTGVSDFEYTPECSVFDLLGIPLYHGWLVDPQSPEAVRAVGKLSYNQLVERIITCKHSSDTNLVTEGLIAEQFLETTAAQLTYHGLCELTAAAKEGELSVFFRNNHFSTMTKHKSHLYLLVTDQGFLQEEQVVWESLHNVDGDSCFCDSDFHLSHSLGKGPGAEGGSGSPEKQLQVDQDYLIALSLQQQQPRGPLGLTDLELGQQLQQEEAQQQQAAQPVRMRTRVLSLQGRGATSGRPAGERRQRPKHESDCILL*

• Vector Type: Bacterial
• DU Number: DU47968
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: please insert two point mutations A416G and Y424A into the DU49563 construct.