cDNA Clone FAM83E

• Plasmid: pGEX6P-1-FAM83E
• Parent Plasmid: pGEX 6P-1
• Expressed: GST-FAM83E
• Genbank: NM_017708.3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGCCTCCCAGCTGGCGGCGCTGGAAGGAGTGGACTCCGGTCCCAGGGTGCCCGGGGCCAGCCCCGGCTTTCTATATTCCGAGGGCCAGCGGCTGGCACTGGAGGCTCTGTTGAGCAAGGGCGCGGAGGCGTTCCAGACCTGCGTGCAGCGCGAGGAGCTGTGGCCCTTCCTCAGTGCGGATGAGGTTCAGGGCTTGGCAGCGGCAGCTGAAGACTGGACAGTGGCCAAGCAGGAGCCCAGCGGGATGGCAGAGGGAGCCACCACCACCGATGTGGACGCGGGCAGCCTGAGCTACTGGCCTGGGCAGTCGGAGCAGCCGGCGCCCGTCCTGCGGCTGGGCTGGCCAGTGGACTCTGCGTGGAAAGGCATCACCCGGGCGCAGCTGTACACCCAGCCTCCTGGAGAGGGTCAGCCGCCCCTCAAGGAGCTGGTGCGGCTGGAGATCCAGGCTGCCCACAAGCTGGTGGCCGTGGTCATGGACGTCTTCACTGACCCAGACCTGCTTTTGGACTTGGTGGATGCTGCCACGCGCCGCTGGGTACCTGTCTACCTGCTCCTGGACCGCCAGCAGCTGCCTGCCTTCCTGGAACTGGCCCAGCAGCTGGGGGTGAACCCCTGGAACACGGAGAACGTGGATGTCCGTGTCGTGCGGGGCTGCAGCTTCCAGAGCCGCTGGCGACGGCAGGTGAGCGGCACCGTGCGGGAGAAGTTTGTGCTGCTGGACGGCGAGAGGGTCATCTCAGGCTCCTACAGCTTCACGTGGAGTGACGCACGCCTGCACCGAGGCCTGGTGACCCTGCTGACTGGTGAAATTGTTGACGCCTTCAGCCTTGAGTTCCGGACGCTGTACGCGGCCTCCTGCCCGCTCCCACCTGCGCCCCCCCAGAAACCCTCGGTCATAGGTGGCCTGCAGCGGGGCCGCAGCCCGCACCGCGTGTCCCGCCGCCGCTCCGTGGCCCCCGCGTCGCCTCCGCCGCCTGACGGCCCGCTGGCCCACCGCCTGGCCGCCTGCCGCGTCTCCCCTGCTACCCCGGGGCCGGCACTCAGTGACATTCTAAGGAGTGTGCAGCGCGCCCGGACCCCCAGCGGCCCCCCGGCCCGGCCCAGCCGCTCCATGTGGGACCTAAGCCGCCTGTCCCAGCTGTCTGGCTCCAGTGATGGGGACAACGAGCTCAAGAAATCCTGGGGCTCCAAGGACACTCCAGCCAAGGCTCTGATGAGGCAGCGGGGCACTGGAGGAGGCCCCTGGGGGGAAGTGGACTCCCGACCTCCGTGGGGCGGTGCCCTGCCCCTGCCCCCCGCCCACCGCCTCCGCTATCTGTCCCCAGCCCGAAGGCGGTTCGGTGGGGATGCTACATTCAAACTTCAAGAGCCCAGAGGCGTCAGGCCGTCAGACTGGGCCCCCCGGGCAGGACTTGGGGGGCAACCCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAASQLAALEGVDSGPRVP
GASPGFLYSEGQRLALEALLSKGAEAFQTCVQREELWPFLSADEVQGLAA
AAEDWTVAKQEPSGMAEGATTTDVDAGSLSYWPGQSEQPAPVLRLGWPVD
SAWKGITRAQLYTQPPGEGQPPLKELVRLEIQAAHKLVAVVMDVFTDPDL
LLDLVDAATRRWVPVYLLLDRQQLPAFLELAQQLGVNPWNTENVDVRVVR
GCSFQSRWRRQVSGTVREKFVLLDGERVISGSYSFTWSDARLHRGLVTLL
TGEIVDAFSLEFRTLYAASCPLPPAPPQKPSVIGGLQRGRSPHRVSRRRS
VAPASPPPPDGPLAHRLAACRVSPATPGPALSDILRSVQRARTPSGPPAR
PSRSMWDLSRLSQLSGSSDGDNELKKSWGSKDTPAKALMRQRGTGGGPWG
EVDSRPPWGGALPLPPAHRLRYLSPARRRFGGDATFKLQEPRGVRPSDWA
PRAGLGGQP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28271
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: contains silent a>c 750bp to KO internal BamHI