cDNA Clone DENND2C

• Plasmid: pCMV5D HA DENND2C
• Parent Plasmid: pCMV5D HA
• Expressed: HA DENND2C
• Genbank: NM_001256404.1
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGATGTTGGTTTTTCTCGTACTACTGTTCAGACACTGTCAAGAAGCCACTGCAAAAACATCAAACAAAAAATTTCTCAATGGGAAGGAAGGGCTAATGGTATATCTAATCCAGAAAAGTGGTGTCCAAAGGACTTTGGAGTGAGATATAACTGTCACCAAGAGATCCGTCTTAAGAAAAATCCTATAGCTGAGAGAAAGAGCAAAAACTTGGATGTAACCAGCCGTGAAAATGTGGGTCTAGATATAAATGAAAATACCAAAAGCCATGATCAAAGTGAGAATGAAAATAAGAAACATGAATATGACGATACACACTTCTTTAAAAATGAATCAGAATCCAACTGGGTATGTTCTCGGGTCAAAGAAATTGAAAGCTGTAAAGAAGATGTCTTAGATCCAGAGACTTCATTACCTCCAGGAAACTTCTATACCTCACAAATACTGTGGAAGAAAATAGAAGCACTTCCCCCAGATAAACTCTTAAATTTGGCTTTAGAACATTGTGACTCTTCAGAAAAAGAACTGAACTTCAGAGTTCTGGATAGTTCATACGGAATAACCAAGAGCTTAGAAAATATTTACTCTGAACCTGAGGGGCAAGAATGTGGACCTTCCATAAATCCTTTGCCAAAACCTCGTAGGACATTCAGATATTTATCCGAATCTGGTGTTACGCCGTATAAAGAAAGAAACTGTGACAAAAAATACTGTGAAAATAACTCTTGTGCACAATCTTCTTTGGCCTCTTCTCAGGAACCTGAACCAAAGAAATATGGTGGAAAAATCAGAGGAAGATCTAAAAGGAAATCCTTTGAATTTGAGGATATTCAGCACTTTCGAAATCGGAACTCACAGACGATTCGTGAAGAACTTGGAAGAAATTCTGGGTCAGCACTTTATTACACACAGTCTGAGGACAATATCTATGAAGATATCATATATCCCACCAAAGAAAATCCATATGAAGATATTCCAGTGCAGCCTTTACCTATGTGGAGATCCCCTTCAGCATGGAAGCTACCACCCGCTAAAAGTGCTTTTAAAGCACCCAAGCTCCCTCCAAAACCTCAGTTCCTTCACCGGAAGACTATGGAAGTAAAGAACTCACAGGCTTATTTGCGGTCAAAGCTTACAAAAGATACAACTTTGCCGGTCACTTTAACGGAATGGAAGCTTTTCCGAGCTGGTGAAGTTGCAAACACGAAAAGGAAAAATCTTCCAAGGCTTGTATTGAAAATAGATGACATATTTGAATCTAAAAGAGGGAAGAAGAAGGTAAAGTTACATTCTTACACTGGAAAGGAATTACCTCCGACAAAAGGTGAAACCAGTGGGAATGAAAGTGATGCCGAGTATCTGCCAAAGAATCGCCATAAACGCTTAGCACAACTGCAACCGTCTTCCAAGAGGAATCCTCACTACCAGACCTTGGAGCGGGATCTTATTGAATTACAGGAGCAGCAGCTGTTTGAACTTTTTGTGGTGGTGTCTCTACAGAAGAAACCATCAGGAATAAGCTATATTCCCCAGGTCATACAACAATTCCCTGGCAAGGATGATCATGGCTATAAGCAGTCCAAAGACATGGAAGAGAGACTTAAAGTTATTCCAAAATTTTGTTTTCCTGATTCAAAGGACTGGATGCCAACCTCAGAACTCAAGAGTGAAACATTCTCCTTTGTCTTGACTGGTGAAGATGGAAGCCGGTGGTTTGGTTACTGTAAGAAGCTCTTGCCAGTAGGCAAAGGAAAGCGACTCCCTGAGGTATACTGCATGGTTAGTCGCCTAGGCTGCTTCAATCTTTTTTCAAAGATTCTGGATGAAGTAGAGAAGAGAAGAGAAATGTCTCCAGCCCTTGTTTACCCATTCATGCGAAGTGTCATGGAAGCTCCTTTCCCAGCTCCTGGACGCACCATCACAGTTAAGAGTTACCTCCCTGGGGCTGGAGATGAGTCCATTGAACTCTGCCGACCACTAGATTCCCGATTGGAACATGTTGATTTTAAATGTCTCTTTAAGTGCCTGAGTGTGTGTCATCTCATCCGGGTCTGTGCCTCTCTCCTTTTGGAGCGTAGGGTAATCTTTGTTGCCAACAGCCTAAGCACCCTGTCAAAATGTGGCCATGCTGTGGTAGCTACACTGTATCCGTTCACCTGGCAGCATACCTATATCCCAGTCCTGCCAGCATCTATGATTGACATCGTGTGCTCACCTACACCATTCCTTATTGGAATCCTGTCTTGCTCCTTACCACAGCTCCAGGACCTACCCATTGAAGAGGTGCTGATAGTTGATCTCTGTGCAGACAAGTTCTTACAGGAGGTATCTGATGAGGATGAAATTCTACCACCAAAACTTCAAGCTGCCCTGATGCAGATTTTGGAAGAACGAAATGAAATCTTGACTCAGGAGCAGAATTTTTCACAAGATGTGACACTCAACTCTCTGGTGTCCGAAGCATTTGTCAGGTTTTTTGTGGAGTTGGTAGGACATTATTCTTTGAACATGACTGTCACTGAGCGTGGGGAGCGTGTTTTCCAAAGGGAACCATTCCGTAAGTCCCACACCTCCCGAAGTGTACGCCACTTCCTGGATCTCTTCATGGAAACTCAGATGTTTGCAGGATTCATCCAGGACCGAGAGCTTCGGAAAAGTGGAGTTAAAGGTTTGTTTGAGATCCGGGCCATCCAGTATTTGGAAACAATTCCTGAGTCGGAGCCCAGTGGGATGAATAGAATTTTGCGGAGTCTTGGAAGCAAAATGAAATTTCTGCAAAAGAAATGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMDVGFSRTTVQTLSRSHCKNIKQKISQWEGRANGISNPEKWCPKDFGVRYNCHQEIRLKKNPIAERKSKNLDVTSRENVGLDINENTKSHDQSENENKKHEYDDTHFFKNESESNWVCSRVKEIESCKEDVLDPETSLPPGNFYTSQILWKKIEALPPDKLLNLALEHCDSSEKELNFRVLDSSYGITKSLENIYSEPEGQECGPSINPLPKPRRTFRYLSESGVTPYKERNCDKKYCENNSCAQSSLASSQEPEPKKYGGKIRGRSKRKSFEFEDIQHFRNRNSQTIREELGRNSGSALYYTQSEDNIYEDIIYPTKENPYEDIPVQPLPMWRSPSAWKLPPAKSAFKAPKLPPKPQFLHRKTMEVKNSQAYLRSKLTKDTTLPVTLTEWKLFRAGEVANTKRKNLPRLVLKIDDIFESKRGKKKVKLHSYTGKELPPTKGETSGNESDAEYLPKNRHKRLAQLQPSSKRNPHYQTLERDLIELQEQQLFELFVVVSLQKKPSGISYIPQVIQQFPGKDDHGYKQSKDMEERLKVIPKFCFPDSKDWMPTSELKSETFSFVLTGEDGSRWFGYCKKLLPVGKGKRLPEVYCMVSRLGCFNLFSKILDEVEKRREMSPALVYPFMRSVMEAPFPAPGRTITVKSYLPGAGDESIELCRPLDSRLEHVDFKCLFKCLSVCHLIRVCASLLLERRVIFVANSLSTLSKCGHAVVATLYPFTWQHTYIPVLPASMIDIVCSPTPFLIGILSCSLPQLQDLPIEEVLIVDLCADKFLQEVSDEDEILPPKLQAALMQILEERNEILTQEQNFSQDVTLNSLVSEAFVRFFVELVGHYSLNMTVTERGERVFQREPFRKSHTSRSVRHFLDLFMETQMFAGFIQDRELRKSGVKGLFEIRAIQYLETIPESEPSGMNRILRSLGSKMKFLQKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU26400
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 300bp missing from EST clone. Gibson used to insert this. silent mutation N447