cDNA Clone DNAJC6

• Plasmid: pcDNA5D FRT/TO HA DNAJC6 c2200t
• Parent Plasmid: pcDNA5D FRT/TO HA
• Expressed: HA DNAJC6 c2200t
• Genbank: NM_001256865.1(DNAJC6):c.2200C>T (p.Gln734Ter)
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCATGGAGCCCAGCTATGGGGGAGGTCTCTTTGACATGGTAAAAGGAGGTGCAGGGAGGCTCTTTAGTAACCTAAAGGACAACTTGAAAGACACCCTCAAAGACACATCTTCTAGAGTGATACAATCTGTGACCAGCTACACAAAGGGAGATTTAGACTTCACTTATGTTACCTCCAGAATTATTGTGATGTCCTTTCCTCTGGACAATGTTGACATAGGATTCAGGAATCAGGTTGATGACATTCGAAGCTTTTTGGATTCCAGACATCTTGACCACTACACAGTATACAATCTGTCACCTAAGTCTTATCGAACTGCCAAGTTTCACAGCCGGGTCTCAGAATGCAGTTGGCCCATTAGGCAGGCTCCCAGTCTGCACAACCTTTTTGCTGTGTGTCGGAATATGTATAACTGGCTACTGCAGAATCCCAAAAATGTCTGTGTTGTCCACTGCTTGGATGGACGGGCGGCATCATCAATTCTGGTTGGTGCTATGTTCATTTTCTGTAATCTCTACTCTACTCCTGGCCCAGCCATTCGATTGCTATATGCAAAGCGACCAGGAATTGGACTTTCACCATCCCATAGGAGATACCTGGGCTATATGTGTGACCTACTGGCAGACAAGCCCTACCGCCCTCACTTCAAGCCTCTCACAATTAAGTCGATCACTGTCAGTCCAATACCCTTTTTCAACAAACAGAGGAATGGATGTCGCCCTTACTGTGATGTACTCATTGGAGAAACCAAAATATATTCGACTTGCACAGATTTTGAACGAATGAAAGAATATCGTGTCCAAGATGGAAAAATCTTCATTCCCTTGAACATCACTGTGCAAGGAGACGTGGTTGTTTCCATGTATCACTTGAGGTCAACCATTGGGAGCCGGCTACAGGCTAAGGTGACCAACACACAGATATTCCAGCTTCAGTTTCACACTGGATTCATACCACTGGACACAACAGTTTTAAAGTTCACCAAGCCTGAGTTAGATGCATGTGATGTACCAGAAAAATATCCTCAGCTATTTCAGGTGACACTGGATGTAGAACTACAGCCCCATGACAAAGTAATAGACTTAACTCCACCATGGGAACATTACTGCACAAAAGATGTCAATCCCAGCATCCTCTTCTCTTCTCACCAGGAACATCAAGATACGCTGGCCTTAGGAGGACAGGCTCCAATAGATATCCCTCCAGACAACCCCAGGCATTACGGACAAAGTGGTTTCTTTGCCTCTCTCTGTTGGCAAGATCAGAAATCGGAGAAGTCATTCTGTGAGGAAGACCACGCTGCCCTAGTGAATCAGGAAAGTGAGCAATCAGATGATGAACTTCTGACACTTTCCAGTCCGCATGGCAATGCCAATGGTGACAAGCCTCATGGAGTCAAGAAGCCCAGCAAAAAGCAGCAGGAGCCAGCAGCCCCTCCACCCCCTGAGGATGTGGACCTTTTGGGCCTGGAAGGGTCTGCAATGAGTAACAGCTTCTCTCCGCCAGCGGCTCCTCCCACCAATTCTGAACTACTGAGTGACCTGTTTGGGGGTGGAGGTGCAGCTGGTCCCACCCAGGCTGGACAGTCAGGAGTGGAAGATGTGTTTCATCCTAGTGGACCTGCGTCTACCCAGTCAACACCACGCCGCTCTGCCACCTCCACCTCTGCGTCTCCAACCCTAAGAGTGGGAGAAGGTGCCACCTTTGACCCATTTGGAGCACCTTCTAAACCATCAGGTCAGGATTTGCTGGGTTCTTTTCTGAACACATCCAGTGCTTCCAGTGACCCCTTTCTCCAGCCCACAAGAAGTCCTTCGCCCACAGTACATGCTTCTAGTACGCCTGCTGTGAACATTCAGCCAGATGTTTCTGGAGGTTGGGACTGGCATGCTAAACCAGGAGGCTTTGGAATGGGAAGCAAGTCAGCTGCCACCAGCCCAACCGGATCCTCGCATGGTACTCCCACCCATCAAAGCAAACCCCAGACTCTGGATCCTTTTGCCGACCTTGGGACACTAGGTAGTTCTTCCTTTGCCAGCAAACCCACCACACCAACTGGATTGGGTGGAGGATTCCCGCCTCTCAGCTCGCCACAGAAGGCGTCTCCCCAGCCTATGGGTGGCGGGTGGCAGCAGGGAGGTGCCTACAACTGGCAGCAGCCACAGCCTAAGCCTCAGCCCAGCATGCCCCACTCCTCTCCCTAGAACCGACCCAACTACAACGTGAGCTTCTCAGCCATGCCTGGGGGCCAGAACGAACGTGGGAAAGGATCAAGTAATTTGGAAGGGAAACAAAAAGCAGCTGATTTTGAAGACCTACTCTCTGGTCAAGGTTTCAATGCTCACAAAGACAAAAAGGGGCCTCGGACAATAGCTGAGATGAGAAAGGAGGAAATGGCCAAGGAAATGGATCCTGAGAAATTAAAGATTCTGGAATGGATTGAAGGCAAAGAAAGAAATATCAGAGCCCTTCTTTCCACGATGCATACCGTACTATGGGCTGGGGAGACCAAGTGGAAACCAGTTGGCATGGCAGACCTGGTAACACCAGAGCAGGTGAAGAAGGTGTACAGGAAGGCTGTCCTGGTGGTGCACCCAGATAAAGCTACTGGGCAACCCTATGAACAATACGCAAAGATGATTTTCATGGAGCTCAATGATGCCTGGTCTGAATTTGAAAACCAAGGCCAAAAGCCCTTATATTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSPGEFMEPSYGGGLFDMVKGGAGRLFSNLKDNLKDTLKDTSSRVIQSVTSYTKGDLDFTYVTSRIIVMSFPLDNVDIGFRNQVDDIRSFLDSRHLDHYTVYNLSPKSYRTAKFHSRVSECSWPIRQAPSLHNLFAVCRNMYNWLLQNPKNVCVVHCLDGRAASSILVGAMFIFCNLYSTPGPAIRLLYAKRPGIGLSPSHRRYLGYMCDLLADKPYRPHFKPLTIKSITVSPIPFFNKQRNGCRPYCDVLIGETKIYSTCTDFERMKEYRVQDGKIFIPLNITVQGDVVVSMYHLRSTIGSRLQAKVTNTQIFQLQFHTGFIPLDTTVLKFTKPELDACDVPEKYPQLFQVTLDVELQPHDKVIDLTPPWEHYCTKDVNPSILFSSHQEHQDTLALGGQAPIDIPPDNPRHYGQSGFFASLCWQDQKSEKSFCEEDHAALVNQESEQSDDELLTLSSPHGNANGDKPHGVKKPSKKQQEPAAPPPPEDVDLLGLEGSAMSNSFSPPAAPPTNSELLSDLFGGGGAAGPTQAGQSGVEDVFHPSGPASTQSTPRRSATSTSASPTLRVGEGATFDPFGAPSKPSGQDLLGSFLNTSSASSDPFLQPTRSPSPTVHASSTPAVNIQPDVSGGWDWHAKPGGFGMGSKSAATSPTGSSHGTPTHQSKPQTLDPFADLGTLGSSSFASKPTTPTGLGGGFPPLSSPQKASPQPMGGGWQQGGAYNWQQPQPKPQPSMPHSSPZNRPNYNVSFSAMPGGQNERGKGSSNLEGKQKAADFEDLLSGQGFNAHKDKKGPRTIAEMRKEEMAKEMDPEKLKILEWIEGKERNIRALLSTMHTVLWAGETKWKPVGMADLVTPEQVKKVYRKAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGQKPLY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU26612
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: mutation at c2200t makes Q734 into a stop.; 2 silent mutations E430 P600