cDNA Clone Pacer/RUBCNL

• Synonyms: C13orf18, KIAA0226L
• Plasmid: pET15b 6HIS SUMO KIAA0226L M1-P440
• Parent Plasmid: pET15b 6His SUMO
• Expressed: 6HIS SUMO KIAA0226L M1-P440
• Genbank: NM_025113.3
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGTGTCACAATCTACAGTCAGGCAGGATTCTCCTGTGGAGCCCTGGGAAGGGATCAGCGATCACTCTGGCATTATTGATGGTTCGCCCAGACTCCTGAACACTGACCATCCTCCTTGCCAATTAGACATCAGGCTCATGAGGCACAAAGCTGTCTGGATTAACCCCCAGGATGTGCAGCAACAGCCGCAGGACTTGCAATCTCAGGTGCCAGCAGCAGGGAACAGTGGGACCCATTTTGTGACAGATGCTGCCTCTCCCTCAGGCCCTTCACCTTCGTGCCTCGGGGACTCCCTGGCAGAGACAACGTTGTCTGAGGATACCACAGACTCCGTTGGCAGCGCTTCTCCCCATGGCTCGAGTGAAAAGAGTAGCAGCTTCTCTCTGTCCTCAACAGAGGTACACATGGTCCGCCCAGGATACTCTCATCGGGTGTCTCTGCCCACAAGCCCTGGGATTTTGGCCACCTCCCCATATCCTGAGACTGACAGTGCTTTTTTTGAGCCTTCCCATCTGACATCTGCTGCTGATGAAGGTGCTGTTCAAGTCAGTAGAAGAACCATTTCTTCGAATTCCTTCTCACCAGAGGTATTTGTGCTGCCTGTTGATGTAGAAAAGGAAAATGCCCACTTTTATGTTGCAGATATGATTATATCAGCAATGGAGAAAATGAAGTGTAACATTCTGAGTCAACAGCAGACAGAGAGCTGGAGTAAAGAAGTCAGTGGGTTACTTGGGAGTGATCAGCCTGACTCTGAAATGACTTTTGATACCAACATAAAGCAAGAGTCTGGGTCTTCTACTTCTTCATACAGTGGCTATGAAGGTTGTGCTGTGTTACAGGTCAGCCCAGTGACTGAAACACGTACTTACCATGATGTGAAAGAGATTTGCAAATGCGATGTTGATGAATTTGTTATTTTAGAGCTTGGAGATTTTAATGATATCACAGAAACCTGTAGCTGTTCCTGCAGCTCCTCTAAGAGTGTCACTTATGAGCCAGACTTCAATTCTGCAGAACTATTAGCCAAAGAGCTGTACCGCGTGTTCCAGAAGTGCTGGATACTGTCAGTAGTTAATTCTCAGCTGGCAGGTTCCCTGAGTGCAGCTGGCTCGATAGTCGTAAATGAAGAGTGTGTCCGAAAAGACTTTGAATCCAGTATGAATGTAGTACAGGAAATTAAATTTAAGTCTAGGATCAGAGGGACTGAAGACTGGGCTCCTCCTAGATTTCAAATCATATTTAATATTCATCCACCACTCAAGAGGGACCTTGTGGTGGCAGCCCAGAATTTTTTCTGTGCCGGCTGTGGAACTCCATGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMVSQSTVRQDSPVEPWEGISDHSGIIDGSPRLLNTDHPPCQLDIRLMRHKAVWINPQDVQQQPQDLQSQVPAAGNSGTHFVTDAASPSGPSPSCLGDSLAETTLSEDTTDSVGSASPHGSSEKSSSFSLSSTEVHMVRPGYSHRVSLPTSPGILATSPYPETDSAFFEPSHLTSAADEGAVQVSRRTISSNSFSPEVFVLPVDVEKENAHFYVADMIISAMEKMKCNILSQQQTESWSKEVSGLLGSDQPDSEMTFDTNIKQESGSSTSSYSGYEGCAVLQVSPVTETRTYHDVKEICKCDVDEFVILELGDFNDITETCSCSCSSSKSVTYEPDFNSAELLAKELYRVFQKCWILSVVNSQLAGSLSAAGSIVVNEECVRKDFESSMNVVQEIKFKSRIRGTEDWAPPRFQIIFNIHPPLKRDLVVAAQNFFCAGCGTP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU26420
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: linker after SENP cleavage deleted