cDNA Clone FAM83G

• Plasmid: pGEX6P-1 FAM83G-6His
• Parent Plasmid: pGEX6P-1
• Expressed: GST-FAM83G-6His
• Genbank: NM_001039999.2
• Tag: GST
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccATGGCCTTCTCTCAGGTGCAGTGTCTGGACGACAACCATGTGAA CTGGCGCTCCAGCGAGTCCAAGCCTGAGTTCTTCTACAGCGAGGAGCAGC GGCTGGCGCTGGAGGCCCTGGTGGCCCGCGGCCGGGACGCCTTCTACGAG GTGCTCAAGCGGGAGAACATCCGAGACTTCCTCTCGGAGCTGGAGCTCAA GCGCATCCTGGAGACCATCGAGGTGTACGACCCGGGCTCTGAGGACCCTC GGGGCACGGGCCCCTCTCAGGGGCCCGAGGACAATGGGGTCGGCGACGGC GAGGAAGCCAGCGGGGCGGATGGGGTCCCCATCGAGGCCGAGCCGCTGCC CTCCCTGGAGTACTGGCCCCAGAAGTCGGACCGCTCCATCCCGCAGCTGG ACCTGGGCTGGCCCGACACCATCGCCTACCGCGGCGTGACCCGGGCTAGC GTCTACATGCAGCCCCCCATAGACGGGCAGGCCCACATCAAAGAGGTGGT GCGGAAGATGATCAGCCAGGCACAGAAGGTGATAGCTGTGGTCATGGACA TGTTCACCGACGTGGACATCTTCAAGGACCTGCTGGACGCCGGCTTCAAG AGGAAAGTGGCCGTGTACATCATCGTGGATGAGAGTAACGTCAAGTACTT CCTGCACATGTGTGAGCGGGCCTGCATGCACCTGGGGCACCTCAAGAATC TCAGAGTGCGGAGCAGCGGGGGAACTGAGTTCTTCACGCGGTCGGCAACC AAGTTCAAGGGTGCCCTGGCCCAGAAGTTCATGTTTGTGGATGGAGACCG GGCTGTGTGCGGCTCCTACAGCTTCACGTGGTCGGCCGCGCGGACGGACC GGAATGTGATCTCTGTGCTGTCTGGCCAGGTGGTGGAGATGTTTGACCGG CAGTTCCAGGAGCTGTACCTCATGTCACACAGTGTGAGCCTCAAGGGCAT CCCTATGGAGAAGGAACCGGAGCCGGAGCCTATTGTGCTGCCCTCTGTGG TCCCCCTGGTGCCCGCGGGCACTGTGGCCAAGAAGCTCGTCAACCCCAAG TACGCACTTGTCAAGGCCAAGAGCGTCGACGAGATTGCCAAGATCTCCTC TGAGAAGCAGGAGGCCAAGAAGCCCCTGGGGCTGAAAGGCCCAGCGCTGG CTGAGCATCCAGGGGAACTCCCCGAGCTGCTGCCACCCATCCACCCAGGA CTGCTTCACCTGGAGAGGGCCAACATGTTTGAGTACCTGCCCACGTGGGT GGAGCCAGACCCGGAGCCTGGCAGCGACATCCTGGGCTACATCAATATCA TCGACCCCAACATCTGGAACCCCCAGCCCAGCCAGATGAACCGCATCAAG ATCCGTGACACCTCCCAGGCCAGCGCCCAGCACCAGCTGTGGAAGCAGAG CCAGGACAGCAGGCCCCGTCCAGAGCCTTGCCCTCCCCCAGAGCCCAGTG CCCCCCAGGACGGTGTCCCAGCTGAGAACGGCCTCCCCCAGGGGGACCCT GAGCCATTGCCCCCCGTGCCCAAGCCCCGGACAGTCCCTGTGGCAGATGT ACTAGCCCGGGACAGCAGTGATATTGGCTGGGTCCTGGAGCTCCCCAAAG AGGAAGCTCCCCAGAATGGGACAGACCATAGGCTACCCAGGATGGCAGGC CCAGGCCACGCCCCACTCCAGCGGCAGCTATCTGTGACCCAGGATGACCC CGAGAGCCTCGGGGTGGGGCTCCCCAATGGGCTGGATGGGGTGGAAGAAG AAGATGATGACGACTACGTAACCCTCAGTGACCAGGACAGCCACTCAGGC AGCTCCGGCCGTGGCCCTGGCCCCCGACGGCCCTCAGTGGCTTCCTCTGT GTCAGAGGAGTACTTCGAGGTGAGAGAGCACTCAGTCCCTCTCCGGAGGC GCCACTCAGAGCAAGTGGCCAACGGGCCAACCCCACCACCGCGCCGGCAG CTGAGTGCCCCCCATATAACCCGAGGGACCTTTGTTGGACCCCAGGGTGG CTCCCCATGGGCCCAGAGTCGGGGAAGAGAAGAAGCAGATGCGTTGAAGA GGATGCAGGCCCAGCGCTCCACAGACAAGGAGGCACAGGGCCAGCAGTTT CATCATCACAGGGTCCCTGCCTCAGGGACTAGGGATAAAGACGGCTTCCC AGGACCCCCTAGGTACCGCTCTGCTGCTGACAGCGTCCAGAGCTCTACCA GAAACGCTGGCCCAGCCATGGCTGGCCCCCACCACTGGCAGGCCAAGGGA GGTCAGGTACCCCGCCTGCTTCCGGAcCCCGGCAGCCCAAGACTGGCCCA AAATGCCCGCCCCATGACCGATGGCAGGGCCACCGAGGAGCATCCGAGTC CCTTCGGAATCCCATACTCCAAACTGTCTCAGTCGAAGCACCTAAAGGCC AGGACGGGCGGTAGCCAGTGGGCCTCATCGGATTCTAAACGGAGGGCTCA AGCCCCCCGGGACCGCAAAGACCCCcatcaccatcaccatcacTAGgcgg ccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAFSQVQCLDDNHVNWRSS
ESKPEFFYSEEQRLALEALVARGRDAFYEVLKRENIRDFLSELELKRILE
TIEVYDPGSEDPRGTGPSQGPEDNGVGDGEEASGADGVPIEAEPLPSLEY
WPQKSDRSIPQLDLGWPDTIAYRGVTRASVYMQPPIDGQAHIKEVVRKMI
SQAQKVIAVVMDMFTDVDIFKDLLDAGFKRKVAVYIIVDESNVKYFLHMC
ERACMHLGHLKNLRVRSSGGTEFFTRSATKFKGALAQKFMFVDGDRAVCG
SYSFTWSAARTDRNVISVLSGQVVEMFDRQFQELYLMSHSVSLKGIPMEK
EPEPEPIVLPSVVPLVPAGTVAKKLVNPKYALVKAKSVDEIAKISSEKQE
AKKPLGLKGPALAEHPGELPELLPPIHPGLLHLERANMFEYLPTWVEPDP
EPGSDILGYINIIDPNIWNPQPSQMNRIKIRDTSQASAQHQLWKQSQDSR
PRPEPCPPPEPSAPQDGVPAENGLPQGDPEPLPPVPKPRTVPVADVLARD
SSDIGWVLELPKEEAPQNGTDHRLPRMAGPGHAPLQRQLSVTQDDPESLG
VGLPNGLDGVEEEDDDDYVTLSDQDSHSGSSGRGPGPRRPSVASSVSEEY
FEVREHSVPLRRRHSEQVANGPTPPPRRQLSAPHITRGTFVGPQGGSPWA
QSRGREEADALKRMQAQRSTDKEAQGQQFHHHRVPASGTRDKDGFPGPPR
YRSAADSVQSSTRNAGPAMAGPHHWQAKGGQVPRLLPDPGSPRLAQNARP
MTDGRATEEHPSPFGIPYSKLSQSKHLKARTGGSQWASSDSKRRAQAPRD
RKDPHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28293
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: internal BamHI killed T2271C silent change