cDNA Clone FAM83D

• Synonyms: spindle protein CHICA
• Plasmid: pCS2+ FAM83D F283A-F287A-cGFP
• Parent Plasmid: pCS2+cGFP
• Expressed: FAM83D F283A-F287A-GFP
• Genbank: Q9H4H8.3 GI:25452904
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGGCTCTGCTGTCCGAGGGCCTGGACGAGGTGCCCGCCGCCTG
CCTGTCGCCGTGCGGGCCGCCCAACCCGACCGAGCTGTTCAGCGAGTCAC
GGCGCCTGGCTCTGGAGGAGCTGGTGGCGGGCGGCCCCGAAGCCTTCGCG
GCCTTCCTGCGACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATGAGGT
GCACGCCATTCTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGG
CGGCGGCGGCGGCCGAGGACTCGTTCGGCTCCTCGCACGACTGCTCTTCG
GGCACCTACTTCCCCGAGCAGTCGGACCTGGAGCCACCGCTGTTGGAGCT
TGGCTGGCCCGCCTTCTACCAGGGCGCCTACCGCGGCGCCACGCGTGTCG
AGACGCACTTCCAGCCCCGCGGCGCTGGCGAAGGTGGCCCCTACGGCTGC
AAGGACGCTCTGCGCCAGCAGCTCCGCTCGGCGCGAGAGGTGATTGCAGT
GGTCATGGACGTGTTCACAGACATCGACATCTTCAGAGACCTGCAAGAAA
TATGCAGGAAACAGGGAGTTGCTGTGTATATCCTTCTGGACCAGGCTCTC
CTCTCTCAATTTCTGGATATGTGCATGGATCTGAAAGTTCATCCTGAACA
GGAAAAGTTAATGACAGTTCGGACTATCACAGGAAATATCTACTATGCAA
GGTCAGGAACTAAGATTATTGGGAAGGTTCACGAAAAGTTCACGTTGATT
GATGGCATCCGCGTGGCAACAGGCTCCTACAGTTTTACATGGACGGATGG
CAAATTAAACAGCAGTAACTTGGTAATTCTGTCTGGCCAAGTGGTTGAAC
ACgcTGATCTGGAggcCCGAATCCTGTATGCCCAGTCCAAGCCCATCAGC
CCCAAACTCCTGTCTCACTTCCAGAGCAGCAACAAGTTTGATCACCTCAC
CAACCGAAAACCACAGTCCAAGGAGCTCACCCTGGGCAACCTGCTGCGGA
TGCGGCTGGCTAGGCTGTCAAGTACTCCCAGGAAGGCGGACCTGGACCCA
GAGATGCCCGCAGAGGGCAAGGCAGAGCGCAAGCCCCATGACTGTGAGTC
CTCTACTGTTAGTGAGGAAGACTACTTCAGCAGCCACAGGGACGAGCTCC
AGAGCAGAAAGGCCATTGACGCTGCCACTCAAACAGAGCCAGGAGAGGAG
ATGCCAGGGCTGAGTGTGAGTGAGGTGGGAACACAAACCAGCATCACCAC
AGCATGTGCTGGTACCCAGACTGCAGTCATCACCAGGATAGCAAGCTCTC
AAACCACGATTTGGTCCAGATCGACCACTACTCAGACTGACATGGATGAG
AACATTCTCTTTCCTCGAGGAACTCAATCTACAGAAGGGTCACCAGTCTC
AAAAATGTCTGTATCGAGATCTTCCAGTTTGAAGTCTTCCTCCTCTGTGT
CTTCCCAAGGCTCTGTGGCAAGCTCCACTGGTTCTCCCGCTTCCATCAGA
ACCACTGACTTCCACAATCCTGGCTATCCCAAGTACCTGGGCACCCCCCA
CCTGGAACTGTACTTGAGTGACTCACTTAGAAACTTGAACAAAGAGCGGC
AATTCCACTTCGCTGGTATCAGGTCCCGGCTCAACCACATGCTGGCTATG
CTGTCAAGGAGAACACTCTTTACTGAAAACCACCTTGGCCTTCATTCTGG
CAATTTCAGCAGAGTTAATTTGCTTGCTGTTAGAGATGTAGCACTTTATC
CTTCCTATCAGGCGGCCGC

Amino Acid Sequence

MALLSEGLDEVPAACLSPCGPPNPTELFSESRRLALEELVAGGPEAFAAF
LRRERLARFLNPDEVHAILRAAERPGEEGAAAAAAAEDSFGSSHDCSSGT
YFPEQSDLEPPLLELGWPAFYQGAYRGATRVETHFQPRGAGEGGPYGCKD
ALRQQLRSAREVIAVVMDVFTDIDIFRDLQEICRKQGVAVYILLDQALLS
QFLDMCMDLKVHPEQEKLMTVRTITGNIYYARSGTKIIGKVHEKFTLIDG
IRVATGSYSFTWTDGKLNSSNLVILSGQVVEHADLEARILYAQSKPISPK
LLSHFQSSNKFDHLTNRKPQSKELTLGNLLRMRLARLSSTPRKADLDPEM
PAEGKAERKPHDCESSTVSEEDYFSSHRDELQSRKAIDAATQTEPGEEMP
GLSVSEVGTQTSITTACAGTQTAVITRIASSQTTIWSRSTTTQTDMDENI
LFPRGTQSTEGSPVSKMSVSRSSSLKSSSSVSSQGSVASSTGSPASIRTT
DFHNPGYPKYLGTPHLELYLSDSLRNLNKERQFHFAGIRSRLNHMLAMLS
RRTLFTENHLGLHSGNFSRVNLLAVRDVALYPSYQAAAMVSKGEELFTGV
VPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLV
TTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAE
VKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI
KVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDP
NEKRDHMVLLEFVTAAGITLGMDELYKSGL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28296
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-terminally truncated predicted variant. Parental 28160 pCS2+-cGFP.