cDNA Clone KIF2A

• Plasmid: pcDNA5D FRT/TO mCherry-KIF2A isoform 1
• Parent Plasmid: pcDNA5D FRT/TO mCherry
• Expressed: mCherry-KIF2A isoform 1
• Genbank: NM_004520.4 → NP_004511.2
• Tag: mCherry
• Species: Human

Sequence of Insert

ggatccATGGCAACGGCCAACTTCGGCAAGATCCAGATCGGGATTTACGT
GGAGATCAAGCGCAGCGATGGCCGAATACATCAAGCAATGGTAACATCTT
TAAATGAAGATAATGAAAGTGTAACTGTTGAATGGATAGAAAATGGAGAT
ACAAAAGGCAAAGAGATTGACCTGGAGAGCATCTTTTCACTTAACCCTGA
CCTTGTTCCTGATGAAGAAATTGAACCCAGTCCAGAAACACCTCCACCTC
CAGCATCCTCAGCCAAAGTAAACAAAATTGTAAAGAATCGACGGACTGTA
GCTTCTATTAAGAATGACCCTCCTTCAAGAGATAATAGAGTGGTTGGTTC
AGCACGTGCACGGCCCAGTCAATTTCCTGAACAGTCTTCCTCTGCACAAC
AGAATGGTAGTGTTTCAGATATATCTCCAGTTCAAGCTGCAAAAAAGGAA
TTTGGACCCCCTTCACGTAGAAAATCTAATTGTGTGAAAGAAGTAGAAAA
ACTGCAAGAAAAACGAGAGAAAAGGAGATTGCAACAGCAAGAACTTAGAG
AAAAAAGAGCCCAGGACGTTGATGCTACAAACCCAAATTATGAAATTATG
TGTATGATCAGAGACTTTAGAGGAAGTTTGGATTATAGACCATTAACAAC
AGCAGATCCTATTGATGAACATAGGATATGTGTGTGTGTAAGAAAACGAC
CACTCAATAAAAAAGAAACTCAAATGAAAGATCTTGATGTAATCACAATT
CCTAGTAAAGATGTTGTGATGGTACATGAACCAAAACAAAAAGTAGATTT
AACAAGGTACCTAGAAAACCAAACATTTCGTTTTGATTATGCCTTTGATG
ACTCAGCTCCTAATGAAATGGTTTACAGGTTTACTGCTAGACCACTAGTG
GAAACTATATTTGAAAGGGGAATGGCTACATGCTTTGCTTATGGGCAGAC
TGGAAGTGGAAAAACTCATACTATGGGTGGTGACTTTTCAGGAAAGAACC
AAGATTGTTCTAAAGGAATTTATGCATTAGCAGCTCGAGATGTCTTTTTA
ATGCTAAAGAAGCCAAACTATAAGAAGCTAGAACTTCAAGTATATGCAAC
CTTCTTTGAAATTTATAGTGGAAAGGTGTTTGACTTGCTAAACAGGAAAA
CAAAATTAAGAGTTCTAGAAGATGGAAAACAGCAGGTTCAAGTGGTGGGA
TTACAGGAACGGGAGGTCAAATGTGTTGAAGATGTACTGAAACTCATTGA
CATAGGCAACAGTTGCAGAACATCCGGTCAAACATCTGCAAATGCACATT
CATCTCGGAGCCATGCAGTGTTTCAGATTATTCTTAGAAGGAAAGGAAAA
CTACATGGCAAATTTTCTCTCATTGATTTGGCTGGAAATGAAAGAGGAGC
TGATACTTCCAGTGCGGACAGGCAAACTAGGCTTGAAGGTGCTGAAATTA
ATAAAAGCCTTTTAGCACTCAAGGAGTGCATCAGAGCCTTAGGTAGAAAT
AAACCTCATACTCCTTTCCGTGCAAGTAAACTCACTCAGGTGTTAAGAGA
TTCTTTCATAGGTGAAAACTCTCGTACCTGCATGATTGCCACAATCTCTC
CAGGAATGGCATCCTGTGAAAATACTCTTAATACATTAAGATATGCAAAT
AGGGTCAAAGAATTGACTGTAGATCCAACTGCTGCTGGTGATGTTCGTCC
AATAATGCACCATCCACCAAACCAGATTGATGACTTAGAGACACAGTGGG
GTGTGGGGAGTTCCCCTCAGAGAGATGATCTAAAACTTCTTTGTGAACAA
AATGAAGAAGAAGTCTCTCCACAGTTGTTTACTTTCCACGAAGCTGTTTC
ACAAATGGTAGAAATGGAAGAACAAGTTGTAGAAGATCACAGGGCAGTGT
TCCAGGAATCTATTCGGTGGTTAGAAGATGAAAAGGCCCTCTTAGAGATG
ACTGAAGAAGTAGATTATGATGTCGATTCATATGCTACACAACTTGAAGC
TATTCTTGAGCAAAAAATAGACATTTTAACTGAACTGCGGGATAAAGTGA
AATCTTTCCGTGCAGCTCTACAAGAGGAGGAACAAGCCAGCAAGCAAATC
AACCCGAAGAGACCCCGTGCCCTTTAAgcggccgc

Amino Acid Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWER
VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA
SSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
NIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKSGLGSMATANFGKI
QIGIYVEIKRSDGRIHQAMVTSLNEDNESVTVEWIENGDTKGKEIDLESI
FSLNPDLVPDEEIEPSPETPPPPASSAKVNKIVKNRRTVASIKNDPPSRD
NRVVGSARARPSQFPEQSSSAQQNGSVSDISPVQAAKKEFGPPSRRKSNC
VKEVEKLQEKREKRRLQQQELREKRAQDVDATNPNYEIMCMIRDFRGSLD
YRPLTTADPIDEHRICVCVRKRPLNKKETQMKDLDVITIPSKDVVMVHEP
KQKVDLTRYLENQTFRFDYAFDDSAPNEMVYRFTARPLVETIFERGMATC
FAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALAARDVFLMLKKPNYKKLE
LQVYATFFEIYSGKVFDLLNRKTKLRVLEDGKQQVQVVGLQEREVKCVED
VLKLIDIGNSCRTSGQTSANAHSSRSHAVFQIILRRKGKLHGKFSLIDLA
GNERGADTSSADRQTRLEGAEINKSLLALKECIRALGRNKPHTPFRASKL
TQVLRDSFIGENSRTCMIATISPGMASCENTLNTLRYANRVKELTVDPTA
AGDVRPIMHHPPNQIDDLETQWGVGSSPQRDDLKLLCEQNEEEVSPQLFT
FHEAVSQMVEMEEQVVEDHRAVFQESIRWLEDEKALLEMTEEVDYDVDSY
ATQLEAILEQKIDILTELRDKVKSFRAALQEEEQASKQINPKRPRAL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28341
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee