cDNA Clone FAM83H

• Plasmid: pcDNA5frtTO GFP FAM83H D236A
• Parent Plasmid: pcDNA5frtTO GFP
• Expressed: FRT-TO-GFP-FAM83H(D236A)
• Genbank: NM_198488.3
• Species: Human

Sequence of Insert

ggatccatggcccgtcgctctcagagctcctcgcagggggacaacccact
ggcacccgggtacctgccgcctcactacaaagagtactaccgcctggcgg
tggatgcactggccgagggtggctcggaggcctacagccgcttcctcgct
accgagggggcaccagacttcctgtgccctgaagagctggaacatgtgag
ccgacaccttcggcctccgcagtatgttacccgagagccacctgaaggca
gccttctcgacgtggacatggatggctcctcgggtacatactggccagtg
aactcagaccaggccgtgcctgagcttgatttgggctggcctctgacctt
cggcttccagggcaccgaggtgaccaccttggtgcagccaccgccccccg
acagccccagtatcaaggatgaggcccgcaggatgatccgttccgcccag
caggtggtggccgtggtgatggacatgttcactgatgtggacctgctcag
cgaagtgctggaggccgcggcccgtcgggtcccagtctacatcctgctgg
atgagatgaacgcgcagcacttcctggacatggccgacaagtgccgtgtc
aacctgcaccacgtggatttcctgcgcgtacggactgtggcgggccccac
ctactactgccgcactgggaagtccttcaagggccacgtcaaggagaagt
tcctgctggtggCctgtgccgtggtgatgagtgggagctacagcttcatg
tggtcctttgagaagatccaccgcagcctggcgcacgtgttccaaggaga
gctggtctccagcttcgacgaggagttccgcatcctcttcgcgcagtccg
agccgcttgtgccctcggccgcggccctggcccgcatggacgcctatgcc
ctggctccgtatgccggggccgggcctctcgtgggcgtccctggggtcgg
ggcgccaacccccttctccttccctaaacgagcgcacctcctgttcccgc
caccccgggaagagggcctgggcttcccctccttcctcgacccggaccgc
cacttcctgtcggccttccgccgggaggagccgccgcggatgccgggggg
cgcgctggaaccgcacgcggggctgcggccActctcgcggcgcctggagg
ccgaggccgggccggctggggagctcgcgggcgcgcggggcttcttccag
gcgcggcacctggagatggacgccttcaagcggcacagcttcgcgaccga
gggcgcgggcgccgtggagaacttcgcggcTgcgcggcaggtgtcgcggc
agacgttcctcagccacggcgacgacttccgcttccagaccagccacttc
caccgtgaccagctctaccagcagcagtaccagtgggacccgcagctcac
gccggcgcgcccgcaaggcctgttcgagaagcttcgcgggggccgcgcgg
gtttcgcggacccggatgacttcaccctgggcgccgggccccgcttcccg
gagctcggacccgacgggcaccagcggctggactacgtgccgtccagcgc
gtcccgcgaggtgcgccacggctcggaccccgccttcgcgcccggacccc
gcggcctggagcccagcggagccccgcgccccaacctgacccagcgcttc
ccatgccaggccgcggcgaggccgggcccagaccccgctcccgaggcgga
gccggagcgcaggggcgggcccgaggggcgggcagggctgcggcgctggc
gtttggcctcctacttgagcggctgccacggcgaggatgggggcgacgac
ggcctaccggcgcccatggaagcggaggcttacgaagacgacgtgctggc
tcccgggggccgggcacctgccggcgacctgctcccctcggccttccgcg
tcccagcagccttccccaccaaggtcccggtgccaggcccgggcagcggc
ggcaacggcccagagcgcgagggcccggaggagcctggcctggccaagca
ggactcattccgctcgcgcctgaaccccctggtccagcgcagctccaggc
tgcgctcctcgctcatcttcagcacgtcacaggccgagggcgcggccggg
gctgcggcggccactgagaaggtgcagctgctgcacaaggagcagacggt
cagcgagacgctggggcccggcggagaggccgtgcgctccgcggcttcca
ccaaggtggcggagctgctggagaagtacaagggcccagcccgtgatccc
ggcggcggcgcgggcgccatcaccgttgccagccacagcaaggccgtcgt
gtcccaggcgtggcgggaagaggtggcggccccaggtgccgtggggggcg
agcgccgcagcctcgagagctgcctgctggacctgcgcgactcctttgca
cagcagctgcaccaggaggcggagcggcagccgggagccgcgtcgctcac
cgcggcgcagctgctcgacacactgggccggagcggctccgaccgcctgc
cttcccgcttcctctctgcccagagccactcaacgtccccgcaagggctg
gacagccctctgccgctggaagggtccggagcgcaccaggtgctccataa
tgagtcaaaagggagccccacctcggcttaccctgagcggaaggggagcc
ccacgcctgggttttccactcgaagaggaagtccaactacaggatttatc
gagcagaaggggagccccacctcagcctaccccgagcgcaggggtagtcc
ggtgccccccgtgccggagcgcaggagcagtccggtgccccccgtgccgg
agcgcaggggcagcctcacccttaccatctccggggagtccccgaaggcc
gggcccgcggaggaggggccgagcggccccatggaagtcctgcgcaaagg
ctccttgcgtcttaggcagctgctgagccccaagggcgagcggcgcatgg
aggatgagggtggcttcccagtgccgcaggagaacggccaacccgagagc
ccgcggcgtctgtcactgggccagggtgacagcacggaggctgccacaga
agagcggggtccgcgggcgcgcctgtcctcagccacggccaacgccttgt
acagcagcaaccttcgggatgacacgaaggccattctggagcagatcagt
gcccacggccagaagcaccgtgcggtccctgccccgagccccggcccgac
ccacaacagccccgagctaggccgtccaccggctgctggcgtcctggccc
cagatatgtccgacaaggacaagtgttcagccatcttccgctcggacagc
ttggggacccagggccggctgagccgcacgctgccagccagcgcggagga
gcgcgatcggctgctgcgccgcatggagagcatgcgcaaggagaagcgcg
tgtacagccgcttcgaggtcttctgcaagaaagaggaggccagcagccct
ggggcaggggaaggccccgcggaggagggcaccagggacagcaaggtggg
caagttcgtgcccaagatcctgggcacgttcaaaagcaagaagtgagcgg
ccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMARRSQ
SSSQGDNPLAPGYLPPHYKEYYRLAVDALAEGGSEAYSRFLATEGAPDFL
CPEELEHVSRHLRPPQYVTREPPEGSLLDVDMDGSSGTYWPVNSDQAVPE
LDLGWPLTFGFQGTEVTTLVQPPPPDSPSIKDEARRMIRSAQQVVAVVMD
MFTDVDLLSEVLEAAARRVPVYILLDEMNAQHFLDMADKCRVNLHHVDFL
RVRTVAGPTYYCRTGKSFKGHVKEKFLLVACAVVMSGSYSFMWSFEKIHR
SLAHVFQGELVSSFDEEFRILFAQSEPLVPSAAALARMDAYALAPYAGAG
PLVGVPGVGAPTPFSFPKRAHLLFPPPREEGLGFPSFLDPDRHFLSAFRR
EEPPRMPGGALEPHAGLRPLSRRLEAEAGPAGELAGARGFFQARHLEMDA
FKRHSFATEGAGAVENFAAARQVSRQTFLSHGDDFRFQTSHFHRDQLYQQ
QYQWDPQLTPARPQGLFEKLRGGRAGFADPDDFTLGAGPRFPELGPDGHQ
RLDYVPSSASREVRHGSDPAFAPGPRGLEPSGAPRPNLTQRFPCQAAARP
GPDPAPEAEPERRGGPEGRAGLRRWRLASYLSGCHGEDGGDDGLPAPMEA
EAYEDDVLAPGGRAPAGDLLPSAFRVPAAFPTKVPVPGPGSGGNGPEREG
PEEPGLAKQDSFRSRLNPLVQRSSRLRSSLIFSTSQAEGAAGAAAATEKV
QLLHKEQTVSETLGPGGEAVRSAASTKVAELLEKYKGPARDPGGGAGAIT
VASHSKAVVSQAWREEVAAPGAVGGERRSLESCLLDLRDSFAQQLHQEAE
RQPGAASLTAAQLLDTLGRSGSDRLPSRFLSAQSHSTSPQGLDSPLPLEG
SGAHQVLHNESKGSPTSAYPERKGSPTPGFSTRRGSPTTGFIEQKGSPTS
AYPERRGSPVPPVPERRSSPVPPVPERRGSLTLTISGESPKAGPAEEGPS
GPMEVLRKGSLRLRQLLSPKGERRMEDEGGFPVPQENGQPESPRRLSLGQ
GDSTEAATEERGPRARLSSATANALYSSNLRDDTKAILEQISAHGQKHRA
VPAPSPGPTHNSPELGRPPAAGVLAPDMSDKDKCSAIFRSDSLGTQGRLS
RTLPASAEERDRLLRRMESMRKEKRVYSRFEVFCKKEEASSPGAGEGPAE
EGTRDSKVGKFVPKILGTFKSKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28428
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent mutations at Pro275 (CCG > CCA) and Ala425 (GCC > GCT)