cDNA Clone CDKL5 (mouse)

• Plasmid: pBABED Puro CDKL5 (mouse) T169E Y171E
• Parent Plasmid: pBabeD puro
• Expressed: CDKL5 (mouse) T169E Y171E
• Species: Mouse

Sequence of Insert

GGATCCATGAAGATTCCTAACATTGGTAATGTGATGAATAAATTTGAGATCCTTGGGGTTGTCGGTGAAGGAGCCTATGGAGTTGTACTTAAATGCAGAC
ACAAGGAAACACATGAAATTGTGGCAATCAAGAAATTCAAGGACAGCGAAGAAAATGAAGAAGTCAAGGAGACGACCTTACGAGAGCTTAAAATGCTTCG
CACTCTCAAGCAGGAAAACATTGTGGAGCTGAAGGAAGCTTTTCGTCGGAGAGGAAAATTATACCTGGTGTTTGAATATGTTGAGAAGAATATGCTTGAA
TTGCTGGAAGAAATGCCAAATGGAGTTCCACCTGAAAAAGTCAAGAGCTATATCTACCAACTAATCAAAGCTATCCACTGGTGCCACAAGAACGACATCG
TCCACAGAGATATAAAGCCTGAAAATCTCTTAATCAGCCACAATGATGTCCTGAAACTATGTGACTTTGGTTTTGCTCGCAATCTCTCAGAGGGCAATAA
TGCTAATTATGAGGAGGAGGTGGCTACAAGGTGGTACCGATCCCCAGAACTGCTACTTGGAGCCCCCTATGGAAAGTCAGTGGACATGTGGTCAGTGGGC
TGTATTCTTGGAGAGCTTAGTGATGGCCAGCCCTTATTTCCTGGAGAAAGTGAAATTGACCAACTCTTTACTATTCAGAAGGTGCTAGGACCACTTCCAT
CTGAGCAAATGAAGCTCTTCTACAGTAACCCTCGTTTCCATGGGCTCCGGTTCCCAGCTGTTAACCATCCTCAGTCATTAGAGAGAAGATACCTTGGAAT
TTTAAATAGTGTTTTACTTGACCTAATGAAGAATTTACTGAAGTTGGACCCAGCTGACAGATACTTGACAGAGCAGTGTTTAAATCATCCTACATTTCAG
ACTCAGAGACTTTTGGACCGGTCTCCTTCAAGATCAACAAAAAGGAAACCTTATCATGTGGAAAGCAGCACCCTGTCTAATAGAAACCAATCCACCAAGG
GTGCTGCCTTACAGACTCACCACAGATCCAACAGCAAGGACATTCAGAACTTAAGTGTGGGTCTGCCCAGGGCTGAGGAAGGCCTGCCTGCAAACGAAAG
CTTCCTCAATGGAAACCTTGCTGGAGCAACTCTCAGCCCAATGCACACCAAAACCTACCAAGCAAGCACCCAGCCTGGCTCTTCCAGCAAAGATCTCACA
AACAACAACATACCACACCTTCTCAGTCCAAAAGAAGCCAAGTCCAAAACAGAGTTTGACTTCAATATTGACACAAAGCCTTCAGAAGGTCCAGGCACAA
AGTACCTCAAGTCCAGCACCAGATCTCAGCAGAACAGGCACTCATTCATGGAAAGCTCCCAGAGCAAAGCTGGGACCCTGCAGCCCAGTGAGAAGCAGAG
TCGGCACAGCTATATTGACACCATTCCCCAGTCCTCCCGGAGTCCATCCTACAGGACAAAGGCCAAAAGCCATGGGGCGTTGAGTGACTCCAAGTCTGTG
AGCAACCTTTCCGAAGCCAGAGCCCAAATCACAGAGACTAATACCAGTAGATACTTCCCATCCAGTTGTTTGGACTTGAACTCTCCCACCAGCCCAACCC
CCACCAGGCACACTGATACGAGAACTTTGCTCAGCCCTTCCGGTCGAAATAATAGAAATGAAGGCACTCTGGACTCACGGCGAACCACCACTAGACACTC
AAAAACCATGGAGGAGTTGAAGCTGCCTGAACACATGGACAGCAGCCATTCGCATTCACTGTCTGCACCTCATGAATCATTTTCTTATGGGCTAGGCTAC
ACCAGCCCCTTCTCCTCTCAGCAGCGTCCTCACAGGCATTCCATGTACGTGACCCGGGACAAAGTGAGAGCGAAAGGCCTTGATGGAAGCTTGAGCATAG
GACAAGGGATGGCAGCCAGAGCCAACAGTTTGCAGCTCTTATCACCCCAGCCTGGAGAACAGCTCCCTCCAGAGATGACTGTGGCAAGACCTTCTGTTAA
AGAGTCCTCCCGAGAAGGCGCCTCTTCATTCCACACCCGTCAGAAGTCTGAGGGTGGAGTATACCATGACCCACACTCTGACGACGGCACAGCTCCCAAA
GAAAATCGGCATCTGTACAATGACCCTGTCCCAAGGAGAGTTGGTAGCTTTTATAGAGTGCCATCTCCACGACCAGACAATTCTTTCCATGAAAATAATG
TGTCCACTCGAGTTTCTTCTCTGCCATCTGATAGCAGTTCTGGTACAAATCATTCAAAAAGACAACCAGGATTTGATCCATGGAAAAGTCCTGAAAACAT
TAGTCATGCCGATCAACTCAAAGAAAAGGAGAAGCAAGGTTTTTTCAGGTCAATGAAAAAGAAAAAGAAGAAAACTCAAACAGTGCCCAACACTGACGGT
CCTGATCTATTGACCTTACAGAAAGCCATTCATTCTTCTAGCACTGCAAGCAGCAGACCAAAGGAGTGGCGACCTGAGAAGCTCTCAGATCTACAGACCC
AGAGCCAACCACTAAAATCTCTTCGAAAGCTGTTGCATCTGTCTTCATCAACCAATCATCCAGCTTCTTCAGATCCTCGGTTCCAGCCCTTAACAGCTCA
ACAAGCCAAAAATTCCTTCTCAGAAATTCGGATTCACCCTCTGAGTCAGGCCACTGGTGGGAGCAGCAACATCCGGCAGGAGCCTACACCAAAAGGCAGA
CCAGCCCTTCAGTTGCCAGGATCTTCCCTCTTAAGGTACAACGGATGGAAACACTCAAGATCAAGATCCAGCCAGCCAGACGAAGTGATCTTCTTGGCAT
CCCATGAGAAATGGAAACAGTGAGCGGCCGC

Amino Acid Sequence

MKIPNIGNVMNKFEILGVVGEGAYGVVLKCRHKETHEIVAIKKFKDSEENEEVKETTLRELKMLRTLKQENIVELKEAFRRRGKLYLVFEYVEKNMLELLEEMPNGVPP
EKVKSYIYQLIKAIHWCHKNDIVHRDIKPENLLISHNDVLKLCDFGFARNLSEGNNANYEEEVATRWYRSPELLLGAPYGKSVDMWSVGCILGELSDGQP
LFPGESEIDQLFTIQKVLGPLPSEQMKLFYSNPRFHGLRFPAVNHPQSLERRYLGILNSVLLDLMKNLLKLDPADRYLTEQCLNHPTFQTQRLLDRSPSR
STKRKPYHVESSTLSNRNQSTKGAALQTHHRSNSKDIQNLSVGLPRAEEGLPANESFLNGNLAGATLSPMHTKTYQASTQPGSSSKDLTNNNIPHLLSPK
EAKSKTEFDFNIDTKPSEGPGTKYLKSSTRSQQNRHSFMESSQSKAGTLQPSEKQSRHSYIDTIPQSSRSPSYRTKAKSHGALSDSKSVSNLSEARAQIT
ETNTSRYFPSSCLDLNSPTSPTPTRHTDTRTLLSPSGRNNRNEGTLDSRRTTTRHSKTMEELKLPEHMDSSHSHSLSAPHESFSYGLGYTSPFSSQQRPH
RHSMYVTRDKVRAKGLDGSLSIGQGMAARANSLQLLSPQPGEQLPPEMTVARPSVKESSREGASSFHTRQKSEGGVYHDPHSDDGTAPKENRHLYNDPVP
RRVGSFYRVPSPRPDNSFHENNVSTRVSSLPSDSSSGTNHSKRQPGFDPWKSPENISHADQLKEKEKQGFFRSMKKKKKKTQTVPNTDGPDLLTLQKAIH
SSSTASSRPKEWRPEKLSDLQTQSQPLKSLRKLLHLSSSTNHPASSDPRFQPLTAQQAKNSFSEIRIHPLSQATGGSSNIRQEPTPKGRPALQLPGSSLL
RYNGWKHSRSRSSQPDEVIFLASHEKWKQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU51742
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee