cDNA Clone SELO

• Plasmid: pMEX3C SELO
• Parent Plasmid: pMEX3C
• Expressed: MBP SELO
• Tag: MBP
• Species: Human

Sequence of Insert

AGATCTATGGCCGTATACAGGGCAGCGCTCGGGGCTTCGCTCGCGGCTGCCCGACTCTTGCCCCTCGGTCGCTGTTCCCCGTCGCCGGCGCCCCGCTCTA
CGTTGTCGGGCGCCGCCATGGAGCCCGCGCCTCGCTGGCTGGCGGGGCTGCGCTTCGACAACCGCGCCCTGCGCGCCCTGCCCGTGGAGGCGCCGCCGCC
CGGTCCCGAGGGCGCCCCGTCCGCGCCGCGGCCCGTGCCCGGGGCCTGCTTCACCCGCGTGCAGCCCACCCCGCTGCGGCAGCCGCGCCTCGTGGCGCTG
TCAGAGCCCGCGCTGGCGTTGCTGGGCCTGGGCGCGCCGCCCGCGCGCGAGGCCGAGGCCGAGGCCGCGCTGTTCTTCAGCGGCAACGCGCTCCTGCCGG
GCGCCGAGCCCGCCGCGCACTGCTACTGCGGCCACCAATTCGGCCAGTTCGCCGGGCAGCTGGGCGACGGCGCCGCCATGTACCTGGGCGAGGTGTGCAC
GGCGACCGGCGAGCGCTGGGAGCTGCAGCTCAAGGGCGCCGGGCCCACGCCCTTCTCCAGACAGGCCGACGGTCGCAAGGTCCTACGGTCAAGCATCCGG
GAGTTTCTATGCAGCGAAGCCATGTTCCACCTGGGAGTCCCCACCACACGGGCCGGCGCCTGCGTCACGTCCGAGTCCACGGTGGTGCGCGACGTGTTCT
ATGATGGTAATCCCAAATATGAACAATGCACGGTTGTGTTGCGTGTAGCTTCCACTTTCATAAGGTTTGGATCCTTTGAGATTTTTAAGTCTGCAGATGA
GCACACAGGGCGTGCAGGCCCCAGCGTGGGGAGGAACGACATTCGAGTGCAGCTGCTCGACTATGTCATCAGCTCCTTTTACCCCGAGATCCAGGCTGCT
CATGCCAGCGACAGCGTGCAGAGAAATGCTGCCTTCTTCCGGGAGGTGACGCGGCGCACGGCGCGGATGGTGGCCGAGTGGCAGTGTGTGGGCTTCTGCC
ACGGCGTGCTCAACACCGACAACATGAGCATCCTGGGGCTCACCATCGACTACGGGCCCTTTGGCTTCCTGGACAGGTACGACCCCGACCACGTGTGCAA
TGCCTCCGACAACACCGGCCGCTACGCGTACAGCAAGCAGCCCGAGGTGTGCAGGTGGAACCTGCGGAAGCTGGCCGAGGCCCTGCAGCCGGAACTGCCC
CTGGAGCTGGGGGAGGCCATCCTGGCCGAGGAGTTTGACGCCGAGTTCCAAAGGCACTACCTGCAGAAGATGCGCAGGAAGCTGGGCCTCGTGCAGGTGG
AGCTGGAGGAAGACGGGGCGCTGGTGTCCAAGCTCCTGGAGACCATGCATCTGACCGGTGCCGACTTCACAAACACCTTCTACTTGCTGAGCTCCTTCCC
AGTGGAGCTAGAGTCGCCAGGCCTGGCGGAATTCCTGGCCAGGCTGATGGAGCAGTGTGCCTCCCTGGAGGAGCTGAGGCTGGCCTTCCGGCCCCAGATG
GATCCCCGGCAGCTATCCATGATGCTGATGCTGGCGCAGTCAAACCCGCAGCTGTTCGCGCTTATGGGCACCCGGGCAGGCATCGCCAGGGAGCTGGAGC
GTGTGGAGCAGCAGTCTCGGCTGGAGCAGCTGAGTGCGGCAGAGCTGCAGAGCAGGAACCAGGGCCACTGGGCTGACTGGCTACAGGCGTACAGAGCCCG
GCTGGACAAGGACCTGGAAGGCGCTGGGGACGCTGCCGCCTGGCAGGCTGAGCACGTGCGCGTGATGCACGCCAACAACCCGAAGTACGTGCTGAGGAAC
TACATCGCGCAGAATGCCATCGAGGCTGCCGAGCGCGGGGACTTCTCAGAGGTGCGGCGGGTGCTGAAACTACTGGAGACCCCTTACCACTGCGAGGCGG
GGGCCGCCACAGACGCCGAGGCCACGGAAGCCGACGGGGCGGACGGCAGGCAGCGCTCCTACAGCAGTAAGCCCCCGCTCTGGGCAGCAGAACTGTGCGT
GACATGATCTTCGTAAGCGGCCGC

Amino Acid Sequence

MMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVR
YNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDL
IKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKP
LGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQG
PLGSMAVYRAALGASLAAARLLPLGRCSPSPAPRSTLSGAAMEPAPRWLAGLRFDNRALRALPVEAPPPGPEGAPSAPRPVPGACFTRVQPTPLRQPRLV
ALSEPALALLGLGAPPAREAEAEAALFFSGNALLPGAEPAAHCYCGHQFGQFAGQLGDGAAMYLGEVCTATGERWELQLKGAGPTPFSRQADGRKVLRSS
IREFLCSEAMFHLGVPTTRAGACVTSESTVVRDVFYDGNPKYEQCTVVLRVASTFIRFGSFEIFKSADEHTGRAGPSVGRNDIRVQLLDYVISSFYPEIQ
AAHASDSVQRNAAFFREVTRRTARMVAEWQCVGFCHGVLNTDNMSILGLTIDYGPFGFLDRYDPDHVCNASDNTGRYAYSKQPEVCRWNLRKLAEALQPE
LPLELGEAILAEEFDAEFQRHYLQKMRRKLGLVQVELEEDGALVSKLLETMHLTGADFTNTFYLLSSFPVELESPGLAEFLARLMEQCASLEELRLAFRP
QMDPRQLSMMLMLAQSNPQLFALMGTRAGIARELERVEQQSRLEQLSAAELQSRNQGHWADWLQAYRARLDKDLEGAGDAAAWQAEHVRVMHANNPKYVL
RNYIAQNAIEAAERGDFSEVRRVLKLLETPYHCEAGAATDAEATEADGADGRQRSYSSKPPLWAAELCVT*SS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51488
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: SELO has a stop codon 3aa from the actual stop which appears to be read through. Lee et al. Characterization of mammalian Selenoprtein O: A Redox-active mitochondrial protein. PLos One 9(4): e95518. doi:10.1371/journal.pone.0095518