cDNA Clone Fam83G

• Plasmid: pcDNA5D FRT/TO C-FLAG FAM83G D125-P823
• Parent Plasmid: pcDNA5D FRT/TO C-FLAG
• Expressed: FAM83G D125-P823-FLAG
• Genbank: NM_001039999.2
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGGACCGCTCCATCCCGCAGCTGGACCTGGGCTGGCCCGACAC
CATCGCCTACCGCGGCGTGACCCGGGCTAGCGTCTACATGCAGCCCCCCA
TAGACGGGCAGGCCCACATCAAAGAGGTGGTGCGGAAGATGATCAGCCAG
GCACAGAAGGTGATAGCTGTGGTCATGGACATGTTCACCGACGTGGACAT
CTTCAAGGACCTGCTGGACGCCGGCTTCAAGAGGAAAGTGGCCGTGTACA
TCATCGTGGATGAGAGTAACGTCAAGTACTTCCTGCACATGTGTGAGCGG
GCCTGCATGCACCTGGGGCACCTCAAGAATCTCAGAGTGCGGAGCAGCGG
GGGAACTGAGTTCTTCACGCGGTCGGCAACCAAGTTCAAGGGTGCCCTGG
CCCAGAAGTTCATGTTTGTGGATGGAGACCGGGCTGTGTGCGGCTCCTAC
AGCTTCACGTGGTCGGCCGCGCGGACGGACCGGAATGTGATCTCTGTGCT
GTCTGGCCAGGTGGTGGAGATGTTTGACCGGCAGTTCCAGGAGCTGTACC
TCATGTCACACAGTGTGAGCCTCAAGGGCATCCCTATGGAGAAGGAACCG
GAGCCGGAGCCTATTGTGCTGCCCTCTGTGGTCCCCCTGGTGCCCGCGGG
CACTGTGGCCAAGAAGCTCGTCAACCCCAAGTACGCACTTGTCAAGGCCA
AGAGCGTCGACGAGATTGCCAAGATCTCCTCTGAGAAGCAGGAGGCCAAG
AAGCCCCTGGGGCTGAAAGGCCCAGCGCTGGCTGAGCATCCAGGGGAACT
CCCCGAGCTGCTGCCACCCATCCACCCAGGACTGCTTCACCTGGAGAGGG
CCAACATGTTTGAGTACCTGCCCACGTGGGTGGAGCCAGACCCGGAGCCT
GGCAGCGACATCCTGGGCTACATCAATATCATCGACCCCAACATCTGGAA
CCCCCAGCCCAGCCAGATGAACCGCATCAAGATCCGTGACACCTCCCAGG
CCAGCGCCCAGCACCAGCTGTGGAAGCAGAGCCAGGACAGCAGGCCCCGT
CCAGAGCCTTGCCCTCCCCCAGAGCCCAGTGCCCCCCAGGACGGTGTCCC
AGCTGAGAACGGCCTCCCCCAGGGGGACCCTGAGCCATTGCCCCCCGTGC
CCAAGCCCCGGACAGTCCCTGTGGCAGATGTACTAGCCCGGGACAGCAGT
GATATTGGCTGGGTCCTGGAGCTCCCCAAAGAGGAAGCTCCCCAGAATGG
GACAGACCATAGGCTACCCAGGATGGCAGGCCCAGGCCACGCCCCACTCC
AGCGGCAGCTATCTGTGACCCAGGATGACCCCGAGAGCCTCGGGGTGGGG
CTCCCCAATGGGCTGGATGGGGTGGAAGAAGAAGATGATGACGACTACGT
AACCCTCAGTGACCAGGACAGCCACTCAGGCAGCTCCGGCCGTGGCCCTG
GCCCCCGACGGCCCTCAGTGGCTTCCTCTGTGTCAGAGGAGTACTTCGAG
GTGAGAGAGCACTCAGTCCCTCTCCGGAGGCGCCACTCAGAGCAAGTGGC
CAACGGGCCAACCCCACCACCGCGCCGGCAGCTGAGTGCCCCCCATATAA
CCCGAGGGACCTTTGTTGGACCCCAGGGTGGCTCCCCATGGGCCCAGAGT
CGGGGAAGAGAAGAAGCAGATGCGTTGAAGAGGATGCAGGCCCAGCGCTC
CACAGACAAGGAGGCACAGGGCCAGCAGTTTCATCATCACAGGGTCCCTG
CCTCAGGGACTAGGGATAAAGACGGCTTCCCAGGACCCCCTAGGTACCGC
TCTGCTGCTGACAGCGTCCAGAGCTCTACCAGAAACGCTGGCCCAGCCAT
GGCTGGCCCCCACCACTGGCAGGCCAAGGGAGGTCAGGTACCCCGCCTGC
TTCCGGACCCCGGCAGCCCAAGACTGGCCCAAAATGCCCGCCCCATGACC
GATGGCAGGGCCACCGAGGAGCATCCGAGTCCCTTCGGAATCCCATACTC
CAAACTGTCTCAGTCGAAGCACCTAAAGGCCAGGACGGGCGGTAGCCAGT
GGGCCTCATCGGATTCTAAACGGAGGGCTCAAGCCCCCCGGGACCGCAAA
GACCCCTAGgcggccgc

Amino Acid Sequence

MDRSIPQLDLGWPDTIAYRGVTRASVYMQPPIDGQAHIKEVVRKMISQAQ
KVIAVVMDMFTDVDIFKDLLDAGFKRKVAVYIIVDESNVKYFLHMCERAC
MHLGHLKNLRVRSSGGTEFFTRSATKFKGALAQKFMFVDGDRAVCGSYSF
TWSAARTDRNVISVLSGQVVEMFDRQFQELYLMSHSVSLKGIPMEKEPEP
EPIVLPSVVPLVPAGTVAKKLVNPKYALVKAKSVDEIAKISSEKQEAKKP
LGLKGPALAEHPGELPELLPPIHPGLLHLERANMFEYLPTWVEPDPEPGS
DILGYINIIDPNIWNPQPSQMNRIKIRDTSQASAQHQLWKQSQDSRPRPE
PCPPPEPSAPQDGVPAENGLPQGDPEPLPPVPKPRTVPVADVLARDSSDI
GWVLELPKEEAPQNGTDHRLPRMAGPGHAPLQRQLSVTQDDPESLGVGLP
NGLDGVEEEDDDDYVTLSDQDSHSGSSGRGPGPRRPSVASSVSEEYFEVR
EHSVPLRRRHSEQVANGPTPPPRRQLSAPHITRGTFVGPQGGSPWAQSRG
REEADALKRMQAQRSTDKEAQGQQFHHHRVPASGTRDKDGFPGPPRYRSA
ADSVQSSTRNAGPAMAGPHHWQAKGGQVPRLLPDPGSPRLAQNARPMTDG
RATEEHPSPFGIPYSKLSQSKHLKARTGGSQWASSDSKRRAQAPRDRKDP
AAADYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28446
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: insert subcloned Bam-Not from source to 41573 parental before mutating out stop codon with oligos as indicated