cDNA Clone Fam83G

• Plasmid: pcDNA5D FRT/TO C-FLAG FAM83G K200-P823
• Parent Plasmid: pcDNA5D FRT/TO C-FLAG
• Expressed: FAM83G K200-P823-FLAG
• Genbank: NM_001039999.2
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGAAAGTGGCCGTGTACATCATCGTGGATGAGAGTAACGTCAA GTACTTCCTGCACATGTGTGAGCGGGCCTGCATGCACCTGGGGCACCTCA AGAATCTCAGAGTGCGGAGCAGCGGGGGAACTGAGTTCTTCACGCGGTCG GCAACCAAGTTCAAGGGTGCCCTGGCCCAGAAGTTCATGTTTGTGGATGG AGACCGGGCTGTGTGCGGCTCCTACAGCTTCACGTGGTCGGCCGCGCGGA CGGACCGGAATGTGATCTCTGTGCTGTCTGGCCAGGTGGTGGAGATGTTT GACCGGCAGTTCCAGGAGCTGTACCTCATGTCACACAGTGTGAGCCTCAA GGGCATCCCTATGGAGAAGGAACCGGAGCCGGAGCCTATTGTGCTGCCCT CTGTGGTCCCCCTGGTGCCCGCGGGCACTGTGGCCAAGAAGCTCGTCAAC CCCAAGTACGCACTTGTCAAGGCCAAGAGCGTCGACGAGATTGCCAAGAT CTCCTCTGAGAAGCAGGAGGCCAAGAAGCCCCTGGGGCTGAAAGGCCCAG CGCTGGCTGAGCATCCAGGGGAACTCCCCGAGCTGCTGCCACCCATCCAC CCAGGACTGCTTCACCTGGAGAGGGCCAACATGTTTGAGTACCTGCCCAC GTGGGTGGAGCCAGACCCGGAGCCTGGCAGCGACATCCTGGGCTACATCA ATATCATCGACCCCAACATCTGGAACCCCCAGCCCAGCCAGATGAACCGC ATCAAGATCCGTGACACCTCCCAGGCCAGCGCCCAGCACCAGCTGTGGAA GCAGAGCCAGGACAGCAGGCCCCGTCCAGAGCCTTGCCCTCCCCCAGAGC CCAGTGCCCCCCAGGACGGTGTCCCAGCTGAGAACGGCCTCCCCCAGGGG GACCCTGAGCCATTGCCCCCCGTGCCCAAGCCCCGGACAGTCCCTGTGGC AGATGTACTAGCCCGGGACAGCAGTGATATTGGCTGGGTCCTGGAGCTCC CCAAAGAGGAAGCTCCCCAGAATGGGACAGACCATAGGCTACCCAGGATG GCAGGCCCAGGCCACGCCCCACTCCAGCGGCAGCTATCTGTGACCCAGGA TGACCCCGAGAGCCTCGGGGTGGGGCTCCCCAATGGGCTGGATGGGGTGG AAGAAGAAGATGATGACGACTACGTAACCCTCAGTGACCAGGACAGCCAC TCAGGCAGCTCCGGCCGTGGCCCTGGCCCCCGACGGCCCTCAGTGGCTTC CTCTGTGTCAGAGGAGTACTTCGAGGTGAGAGAGCACTCAGTCCCTCTCC GGAGGCGCCACTCAGAGCAAGTGGCCAACGGGCCAACCCCACCACCGCGC CGGCAGCTGAGTGCCCCCCATATAACCCGAGGGACCTTTGTTGGACCCCA GGGTGGCTCCCCATGGGCCCAGAGTCGGGGAAGAGAAGAAGCAGATGCGT TGAAGAGGATGCAGGCCCAGCGCTCCACAGACAAGGAGGCACAGGGCCAG CAGTTTCATCATCACAGGGTCCCTGCCTCAGGGACTAGGGATAAAGACGG CTTCCCAGGACCCCCTAGGTACCGCTCTGCTGCTGACAGCGTCCAGAGCT CTACCAGAAACGCTGGCCCAGCCATGGCTGGCCCCCACCACTGGCAGGCC AAGGGAGGTCAGGTACCCCGCCTGCTTCCGGACCCCGGCAGCCCAAGACT GGCCCAAAATGCCCGCCCCATGACCGATGGCAGGGCCACCGAGGAGCATC CGAGTCCCTTCGGAATCCCATACTCCAAACTGTCTCAGTCGAAGCACCTA AAGGCCAGGACGGGCGGTAGCCAGTGGGCCTCATCGGATTCTAAACGGAG GGCTCAAGCCCCCCGGGACCGCAAAGACCCCTAGgcggccgc

Amino Acid Sequence

MKVAVYIIVDESNVKYFLHMCERACMHLGHLKNLRVRSSGGTEFFTRSAT
KFKGALAQKFMFVDGDRAVCGSYSFTWSAARTDRNVISVLSGQVVEMFDR
QFQELYLMSHSVSLKGIPMEKEPEPEPIVLPSVVPLVPAGTVAKKLVNPK
YALVKAKSVDEIAKISSEKQEAKKPLGLKGPALAEHPGELPELLPPIHPG
LLHLERANMFEYLPTWVEPDPEPGSDILGYINIIDPNIWNPQPSQMNRIK
IRDTSQASAQHQLWKQSQDSRPRPEPCPPPEPSAPQDGVPAENGLPQGDP
EPLPPVPKPRTVPVADVLARDSSDIGWVLELPKEEAPQNGTDHRLPRMAG
PGHAPLQRQLSVTQDDPESLGVGLPNGLDGVEEEDDDDYVTLSDQDSHSG
SSGRGPGPRRPSVASSVSEEYFEVREHSVPLRRRHSEQVANGPTPPPRRQ
LSAPHITRGTFVGPQGGSPWAQSRGREEADALKRMQAQRSTDKEAQGQQF
HHHRVPASGTRDKDGFPGPPRYRSAADSVQSSTRNAGPAMAGPHHWQAKG
GQVPRLLPDPGSPRLAQNARPMTDGRATEEHPSPFGIPYSKLSQSKHLKA
RTGGSQWASSDSKRRAQAPRDRKDPAAADYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28448
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: insert subcloned Bam-Not from source to 41573 parental before mutating out stop codon with oligos as indicated