cDNA Clone Fam83G

• Plasmid: pcDNA5D FRT/TO C-FLAG FAM83G W275-P823
• Parent Plasmid: pcDNA5D FRT/TO C-FLAG
• Expressed: FAM83G W275-P823-FLAG
• Genbank: NM_001039999.2
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGTGGTCGGCCGCGCGGACGGACCGGAATGTGATCTCTGTGCT GTCTGGCCAGGTGGTGGAGATGTTTGACCGGCAGTTCCAGGAGCTGTACC TCATGTCACACAGTGTGAGCCTCAAGGGCATCCCTATGGAGAAGGAACCG GAGCCGGAGCCTATTGTGCTGCCCTCTGTGGTCCCCCTGGTGCCCGCGGG CACTGTGGCCAAGAAGCTCGTCAACCCCAAGTACGCACTTGTCAAGGCCA AGAGCGTCGACGAGATTGCCAAGATCTCCTCTGAGAAGCAGGAGGCCAAG AAGCCCCTGGGGCTGAAAGGCCCAGCGCTGGCTGAGCATCCAGGGGAACT CCCCGAGCTGCTGCCACCCATCCACCCAGGACTGCTTCACCTGGAGAGGG CCAACATGTTTGAGTACCTGCCCACGTGGGTGGAGCCAGACCCGGAGCCT GGCAGCGACATCCTGGGCTACATCAATATCATCGACCCCAACATCTGGAA CCCCCAGCCCAGCCAGATGAACCGCATCAAGATCCGTGACACCTCCCAGG CCAGCGCCCAGCACCAGCTGTGGAAGCAGAGCCAGGACAGCAGGCCCCGT CCAGAGCCTTGCCCTCCCCCAGAGCCCAGTGCCCCCCAGGACGGTGTCCC AGCTGAGAACGGCCTCCCCCAGGGGGACCCTGAGCCATTGCCCCCCGTGC CCAAGCCCCGGACAGTCCCTGTGGCAGATGTACTAGCCCGGGACAGCAGT GATATTGGCTGGGTCCTGGAGCTCCCCAAAGAGGAAGCTCCCCAGAATGG GACAGACCATAGGCTACCCAGGATGGCAGGCCCAGGCCACGCCCCACTCC AGCGGCAGCTATCTGTGACCCAGGATGACCCCGAGAGCCTCGGGGTGGGG CTCCCCAATGGGCTGGATGGGGTGGAAGAAGAAGATGATGACGACTACGT AACCCTCAGTGACCAGGACAGCCACTCAGGCAGCTCCGGCCGTGGCCCTG GCCCCCGACGGCCCgCAGTGGCTTCCTCTGTGTCAGAGGAGTACTTCGAG GTGAGAGAGCACTCAGTCCCTCTCCGGAGGCGCCACTCAGAGCAAGTGGC CAACGGGCCAACCCCACCACCGCGCCGGCAGCTGAGTGCCCCCCATATAA CCCGAGGGACCTTTGTTGGACCCCAGGGTGGCTCCCCATGGGCCCAGAGT CGGGGAAGAGAAGAAGCAGATGCGTTGAAGAGGATGCAGGCCCAGCGCTC CACAGACAAGGAGGCACAGGGCCAGCAGTTTCATCATCACAGGGTCCCTG CCTCAGGGACTAGGGATAAAGACGGCTTCCCAGGACCCCCTAGGTACCGC TCTGCTGCTGACAGCGTCCAGAGCTCTACCAGAAACGCTGGCCCAGCCAT GGCTGGCCCCCACCACTGGCAGGCCAAGGGAGGTCAGGTACCCCGCCTGC TTCCGGACCCCGGCAGCCCAAGACTGGCCCAAAATGCCCGCCCCATGACC GATGGCAGGGCCACCGAGGAGCATCCGAGTCCCTTCGGAATCCCATACTC CAAACTGTCTCAGTCGAAGCACCTAAAGGCCAGGACGGGCGGTAGCCAGT GGGCCTCATCGGATTCTAAACGGAGGGCTCAAGCCCCCCGGGACCGCAAA GACCCCTAGgcggccgc

Amino Acid Sequence

MWSAARTDRNVISVLSGQVVEMFDRQFQELYLMSHSVSLKGIPMEKEPEP
EPIVLPSVVPLVPAGTVAKKLVNPKYALVKAKSVDEIAKISSEKQEAKKP
LGLKGPALAEHPGELPELLPPIHPGLLHLERANMFEYLPTWVEPDPEPGS
DILGYINIIDPNIWNPQPSQMNRIKIRDTSQASAQHQLWKQSQDSRPRPE
PCPPPEPSAPQDGVPAENGLPQGDPEPLPPVPKPRTVPVADVLARDSSDI
GWVLELPKEEAPQNGTDHRLPRMAGPGHAPLQRQLSVTQDDPESLGVGLP
NGLDGVEEEDDDDYVTLSDQDSHSGSSGRGPGPRRPAVASSVSEEYFEVR
EHSVPLRRRHSEQVANGPTPPPRRQLSAPHITRGTFVGPQGGSPWAQSRG
REEADALKRMQAQRSTDKEAQGQQFHHHRVPASGTRDKDGFPGPPRYRSA
ADSVQSSTRNAGPAMAGPHHWQAKGGQVPRLLPDPGSPRLAQNARPMTDG
RATEEHPSPFGIPYSKLSQSKHLKARTGGSQWASSDSKRRAQAPRDRKDP
AAADYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28452
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: insert subcloned Bam-Not from source to 41573 parental before mutating out stop codon with oligos as indicated. **Clone has S610A mutation to be corrected**