cDNA Clone FAM83D

• Plasmid: pBabe-FAM83D (shorter variant)
• Parent Plasmid: pBabe puro DU
• Expressed: FAM83D
• Species: Human

Sequence of Insert

ggatccATGGCTCTGCTGTCCGAGGGCCTGGACGAGGTGCCCGCCGCCTG
CCTGTCGCCGTGCGGGCCGCCCAACCCGACCGAGCTGTTCAGCGAGTCAC
GGCGCCTGGCTCTGGAGGAGCTGGTGGCGGGCGGCCCCGAAGCCTTCGCG
GCCTTCCTGCGACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATGAGGT
GCACGCCATTCTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGG
CGGCGGCGGCGGCCGAGGACTCGTTCGGCTCCTCGCACGACTGCTCTTCG
GGCACCTACTTCCCCGAGCAGTCGGACCTGGAGCCACCGCTGTTGGAGCT
TGGCTGGCCCGCCTTCTACCAGGGCGCCTACCGCGGCGCCACGCGTGTCG
AGACGCACTTCCAGCCCCGCGGCGCTGGCGAAGGTGGCCCCTACGGCTGC
AAGGACGCTCTGCGCCAGCAGCTCCGCTCGGCGCGAGAGGTGATTGCAGT
GGTCATGGACGTGTTCACAGACATCGACATCTTCAGAGACCTGCAAGAAA
TATGCAGGAAACAGGGAGTTGCTGTGTATATCCTTCTGGACCAGGCTCTC
CTCTCTCAATTTCTGGATATGTGCATGGATCTGAAAGTTCATCCTGAACA
GGAAAAGTTAATGACAGTTCGGACTATCACAGGAAATATCTACTATGCAA
GGTCAGGAACTAAGATTATTGGGAAGGTTCACGAAAAGTTCACGTTGATT
GATGGCATCCGCGTGGCAACAGGCTCCTACAGTTTTACATGGACGGATGG
CAAATTAAACAGCAGTAACTTGGTAATTCTGTCTGGCCAAGTGGTTGAAC
ACTTTGATCTGGAATTCCGAATCCTGTATGCCCAGTCCAAGCCCATCAGC
CCCAAACTCCTGTCTCACTTCCAGAGCAGCAACAAGTTTGATCACCTCAC
CAACCGAAAACCACAGTCCAAGGAGCTCACCCTGGGCAACCTGCTGCGGA
TGCGGCTGGCTAGGCTGTCAAGTACTCCCAGGAAGGCGGACCTGGACCCA
GAGATGCCCGCAGAGGGCAAGGCAGAGCGCAAGCCCCATGACTGTGAGTC
CTCTACTGTTAGTGAGGAAGACTACTTCAGCAGCCACAGGGACGAGCTCC
AGAGCAGAAAGGCCATTGACGCTGCCACTCAAACAGAGCCAGGAGAGGAG
ATGCCAGGGCTGAGTGTGAGTGAGGTGGGAACACAAACCAGCATCACCAC
AGCATGTGCTGGTACCCAGACTGCAGTCATCACCAGGATAGCAAGCTCTC
AAACCACGATTTGGTCCAGATCGACCACTACTCAGACTGACATGGATGAG
AACATTCTCTTTCCTCGAGGAACTCAATCTACAGAAGGGTCACCAGTCTC
AAAAATGTCTGTATCGAGATCTTCCAGTTTGAAGTCTTCCTCCTCTGTGT
CTTCCCAAGGCTCTGTGGCAAGCTCCACTGGTTCTCCCGCTTCCATCAGA
ACCACTGACTTCCACAATCCTGGCTATCCCAAGTACCTGGGCACCCCCCA
CCTGGAACTGTACTTGAGTGACTCACTTAGAAACTTGAACAAAGAGCGGC
AATTCCACTTCGCTGGTATCAGGTCCCGGCTCAACCACATGCTGGCTATG
CTGTCAAGGAGAACACTCTTTACTGAAAACCACCTTGGCCTTCATTCTGG
CAATTTCAGCAGAGTTAATTTGCTTGCTGTTAGAGATGTAGCACTTTATC
CTTCCTATCAGTAAgtcgac

Amino Acid Sequence

MALLSEGLDEVPAACLSPCGPPNPTELFSESRRLALEELVAGGPEAFAAF
LRRERLARFLNPDEVHAILRAAERPGEEGAAAAAAAEDSFGSSHDCSSGT
YFPEQSDLEPPLLELGWPAFYQGAYRGATRVETHFQPRGAGEGGPYGCKD
ALRQQLRSAREVIAVVMDVFTDIDIFRDLQEICRKQGVAVYILLDQALLS
QFLDMCMDLKVHPEQEKLMTVRTITGNIYYARSGTKIIGKVHEKFTLIDG
IRVATGSYSFTWTDGKLNSSNLVILSGQVVEHFDLEFRILYAQSKPISPK
LLSHFQSSNKFDHLTNRKPQSKELTLGNLLRMRLARLSSTPRKADLDPEM
PAEGKAERKPHDCESSTVSEEDYFSSHRDELQSRKAIDAATQTEPGEEMP
GLSVSEVGTQTSITTACAGTQTAVITRIASSQTTIWSRSTTTQTDMDENI
LFPRGTQSTEGSPVSKMSVSRSSSLKSSSSVSSQGSVASSTGSPASIRTT
DFHNPGYPKYLGTPHLELYLSDSLRNLNKERQFHFAGIRSRLNHMLAMLS
RRTLFTENHLGLHSGNFSRVNLLAVRDVALYPSYQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28498
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: delta aaM1-Ala30 with respect to full-length NCBI ref. N-terminally truncated predicted variant. Silent change at Glu316 (GAG > GAA)
• Low Temp: No