cDNA Clone FAM83E

• Plasmid: pcDNA5-FRT/TO-GFP FAM83E D243A
• Parent Plasmid: pcDNA5-FRT/TO-GFP
• Expressed: GFP-FAM83E (D243A)
• Genbank: NM_017708.3
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCGGCCTCCCAGCTGGCGGCGCTGGAAGGAGTGGACTCCGG
TCCCAGGGTGCCCGGGGCCAGCCCCGGCTTTCTATATTCCGAGGGCCAGC
GGCTGGCACTGGAGGCTCTGTTGAGCAAGGGCGCGGAGGCGTTCCAGACC
TGCGTGCAGCGCGAGGAGCTGTGGCCCTTCCTCAGTGCGGATGAGGTTCA
GGGCTTGGCAGCGGCAGCTGAAGACTGGACAGTGGCCAAGCAGGAGCCCA
GCGGGATGGCAGAGGGAGCCACCACCACCGATGTGGACGCGGGCAGCCTG
AGCTACTGGCCTGGGCAGTCGGAGCAGCCGGCGCCCGTCCTGCGGCTGGG
CTGGCCAGTGGACTCTGCGTGGAAAGGCATCACCCGGGCGCAGCTGTACA
CCCAGCCTCCTGGAGAGGGTCAGCCGCCCCTCAAGGAGCTGGTGCGGCTG
GAGATCCAGGCTGCCCACAAGCTGGTGGCCGTGGTCATGGACGTCTTCAC
TGACCCAGACCTGCTTTTGGACTTGGTGGATGCTGCCACGCGCCGCTGGG
TACCTGTCTACCTGCTCCTGGACCGCCAGCAGCTGCCTGCCTTCCTGGAA
CTGGCCCAGCAGCTGGGGGTGAACCCCTGGAACACGGAGAACGTGGATGT
CCGTGTCGTGCGGGGCTGCAGCTTCCAGAGCCGCTGGCGACGGCAGGTGA
GCGGCACCGTGCGGGAGAAGTTTGTGCTGCTGGcCGGCGAGAGGGTCATC
TCAGGCTCCTACAGCTTCACGTGGAGTGACGCACGCCTGCACCGAGGCCT
GGTGACCCTGCTGACTGGTGAAATTGTTGACGCCTTCAGCCTTGAGTTCC
GGACGCTGTACGCGGCCTCCTGCCCGCTCCCACCTGCGCCCCCCCAGAAA
CCCTCGGTCATAGGTGGCCTGCAGCGGGGCCGCAGCCCGCACCGCGTGTC
CCGCCGCCGCTCCGTGGCCCCCGCGTCGCCTCCGCCGCCTGACGGCCCGC
TGGCCCACCGCCTGGCCGCCTGCCGCGTCTCCCCTGCTACCCCGGGGCCG
GCACTCAGTGACATTCTAAGGAGTGTGCAGCGCGCCCGGACCCCCAGCGG
CCCCCCGGCCCGGCCCAGCCGCTCCATGTGGGACCTAAGCCGCCTGTCCC
AGCTGTCTGGCTCCAGTGATGGGGACAACGAGCTCAAGAAATCCTGGGGC
TCCAAGGACACTCCAGCCAAGGCTCTGATGAGGCAGCGGGGCACTGGAGG
AGGCCCCTGGGGGGAAGTGGACTCCCGACCTCCGTGGGGCGGTGCCCTGC
CCCTGCCCCCCGCCCACCGCCTCCGCTATCTGTCCCCAGCCCGAAGGCGG
TTCGGTGGGGATGCTACATTCAAACTTCAAGAGCCCAGAGGCGTCAGGCC
GTCAGACTGGGCCCCCCGGGCAGGACTTGGGGGGCAACCCTGAGCGGCCG
C

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAASQL
AALEGVDSGPRVPGASPGFLYSEGQRLALEALLSKGAEAFQTCVQREELW
PFLSADEVQGLAAAAEDWTVAKQEPSGMAEGATTTDVDAGSLSYWPGQSE
QPAPVLRLGWPVDSAWKGITRAQLYTQPPGEGQPPLKELVRLEIQAAHKL
VAVVMDVFTDPDLLLDLVDAATRRWVPVYLLLDRQQLPAFLELAQQLGVN
PWNTENVDVRVVRGCSFQSRWRRQVSGTVREKFVLLAGERVISGSYSFTW
SDARLHRGLVTLLTGEIVDAFSLEFRTLYAASCPLPPAPPQKPSVIGGLQ
RGRSPHRVSRRRSVAPASPPPPDGPLAHRLAACRVSPATPGPALSDILRS
VQRARTPSGPPARPSRSMWDLSRLSQLSGSSDGDNELKKSWGSKDTPAKA
LMRQRGTGGGPWGEVDSRPPWGGALPLPPAHRLRYLSPARRRFGGDATFK
LQEPRGVRPSDWAPRAGLGGQP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28481
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent A>C 750 bp to KO internal BamHI