cDNA Clone CK1α

• Synonyms: Casein kinase I isoform alpha
• Plasmid: pcDNA5-FRT/TO C-mCherry DU-CK1 alpha
• Parent Plasmid: pcDNA5-FRT/TO C-mCherry DU
• Expressed: CK1 alpha-C-mCHERRY
• Genbank: P48729.2
• Tag: mCherry
• Species: Human

Sequence of Insert

GGATCCATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTCGGAGG
GAAATATAAACTGGTACGGAAGATCGGGTCTGGCTCCTTCGGGGACATCT
ATTTGGCGATCAACATCACCAACGGCGAGGAAGTGGCAGTGAAGCTAGAA
TCTCAGAAGGCCAGGCATCCCCAGTTGCTGTACGAGAGCAAGCTCTATAA
GATTCTTCAAGGTGGGGTTGGCATCCCCCACATACGGTGGTATGGTCAGG
AAAAAGACTACAATGTACTAGTCATGGATCTTCTGGGACCTAGCCTCGAA
GACCTCTTCAATTTCTGTTCAAGAAGGTTCACAATGAAAACTGTACTTAT
GTTAGCTGACCAGATGATCAGTAGAATTGAATATGTGCATACAAAGAATT
TTATACACAGAGACATTAAACCAGATAACTTCCTAATGGGTATTGGGCGT
CACTGTAATAAGTTATTCCTTATTGATTTTGGTTTGGCCAAAAAGTACAG
AGACAACAGGACAAGGCAACACATACCATACAGAGAAGATAAAAACCTCA
CTGGCACTGCCCGATATGCTAGCATCAATGCACATCTTGGTATTGAGCAG
AGTCGCCGAGATGACATGGAATCATTAGGATATGTTTTGATGTATTTTAA
TAGAACCAGCCTGCCATGGCAAGGGCTAAAGGCTGCAACAAAGAAACAAA
AATATGAAAAGATTAGTGAAAAGAAGATGTCCACGCCTGTTGAAGTTTTA
TGTAAGGGGTTTCCTGCAGAATTTGCGATGTACTTAAACTATTGTCGTGG
GCTACGCTTTGAGGAAGCCCCAGATTACATGTATCTGAGGCAGCTATTCC
GCATTCTTTTCAGGACCCTGAACCATCAATATGACTACACATTTGATTGG
ACAATGTTAAAGCAGAAAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCA
GGGTCAGCAGGCCCAAACCCCCACAGGCAAGCAAACTGACAAAACCAAGA
GTAACATGAAAGGTTTCGCGGCCGC

Amino Acid Sequence

MASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQ
KARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDL
FNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHC
NKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSR
RDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCK
GFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTM
LKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGFAAAMVSKGEEDNM
AIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLP
FAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHN
EDYTIVEQYERAEGRHSTGGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28469
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 23848. Tyr17 and Thr330 restored (NCBI update)