cDNA Clone MCM5 (mouse)

• Plasmid: pMEX3C MCM5 (mouse) 1-222
• Parent Plasmid: pMEX3C
• Expressed: MBP MCM5 (mouse) 1-222
• Genbank: NP_001289469.1
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCTATGTCGGGCTTCGACGACCCGGGCATTTTCTACAGCGACAGCTTCGGTGGCGACCCCGGTGCGGAAGAGGGCCAGGCCCGCAAGTCGCAACTGC
AGAGGCGATTCAAGGAGTTCCTGAGACAGTACCGAGTGGGCACCGATCGCACGGGCTTCACCTTCAAGTACAGAGATGAACTCAAGCGGCATTACAACCT
GGGTGAATACTGGATCGAGGTGGAGATGGAGGACCTGGCCAGTTTTGACGAGGAACTGGCTGACCACTTGCATAAACAGCCGGCCGAGCACTTACAGCTG
CTTGAGGAAGCTGCCAAGGAGGTGGCAGATGAGGTGACCCGGCCCCGGCCAGCTGGAGATGAGCTGCTCCAAGACATCCAGGTCATGCTCAAGTCAGATG
CCAGCCCGTCGAGCATTCGGATTCTGAAGTCAGACATGATGTCACACCTGGTGAAGATCCCTGGCATCATCATTTCAGCCTCTGCAGTCCGTGCCAAGGC
TACTCGTATCTCCATTCAGTGCCGCAGCTGCCACAACACCCTCACCAATATCGCCATGCGCCCAGGCCTAGAGGGCTATGCCCTTCCCAGGAAGTGCAAT
ATGGATCAGGCTGGGCGCCCAAAGTGCCCACTGGATCCATACTTCATCATGCCTGACAAGTGCAAGTGTGTGTAAGCGGCCGC

Amino Acid Sequence

MMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVR
YNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDL
IKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKP
LGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQG
PLGSMSGFDDPGIFYSDSFGGDPGAEEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELKRHYNLGEYWIEVEMEDLASFDEELADHLHKQPAEHL
QLLEEAAKEVADEVTRPRPAGDELLQDIQVMLKSDASPSSIRILKSDMMSHLVKIPGIIISASAVRAKATRISIQCRSCHNTLTNIAMRPGLEGYALPRK
CNMDQAGRPKCPLDPYFIMPDKCKCV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51834
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make a construct of M. musculus Mcm5 (1-222), for expression in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in SHEEP.