cDNA Clone MCM3 (mouse)

• Plasmid: pGEX6P1 MCM3 (mouse)1-222
• Parent Plasmid: pGEX6P1
• Expressed: GST MCM3 (mouse) 1-222
• Genbank: NP_032589.1
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGGCGGGCACAGTAGTGCTGGATGATGTGGAGCTGCGGGAAGCGCAGAGAGACTACTTGGACTTCCTGGATGACGAGGAGGACCAAGGCATTT
ACCAGAACAAGGTTCGGGAACTGATCAGTGACAACCAGTATCGGCTGATTGTCAGCGTGAATGACCTGCGCAGGAAGAATGAAAAGAGGGCTAACCGCCT
CCTGAACAATGCGTTTGAGGAGCTGGTTGCCTTCCAGCGGGCCTTGAAGGATTTCGTGGCCTCCATTGACGCCACCTACGCCAAGCAGTATGAGGAGTTC
TACATAGGATTGGAAGGCAGCTTTGGCTCAAAGCACGTTTCTCCCCGGACTCTCACTTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAAGGCATTGTTA
CTAAATGCTCTCTGGTTCGCCCCAAAGTCGTTCGCAGTGTTCACTACTGTCCTGCTACTAAGAAGACCATAGAGCGACGCTACTCTGACCTCACCACCCT
AGTGGCTTTCCCATCCAGTTCTGTTTATCCCACAAAGGATGAGGAGAACAACCCCCTGGAGACAGAGTATGGCCTGTCTGTCTACAAGGACCACCAGACC
ATCACCATCCAGGAGATGCCAGAGAAGGCCCCTGCTGGTCAGCTCCCTCGCTCTGTGGATGTCATTCTAGACTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQNKVRELISDNQYRLIVSVNDLRRKNEKRANRLL
NNAFEELVAFQRALKDFVASIDATYAKQYEEFYIGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPKVVRSVHYCPATKKTIERRYSDLTTLV
AFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRSVDVILD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51847
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make a construct of M. musculus Mcm3 (1-222), for expression in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in SHEEP.