cDNA Clone RNF216

• Plasmid: pcDNA5D FRT/TO Flag RNF216 P826A P827A codon opt
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG RNF216 P826A P827A
• Genbank: NM_207111.3
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGAAGAGGGCAACAACAACGAGGAAGTGATCCACCTGAACAACTTCCACTGCCACCGGGGCCAAGAGTGGATCAACCTGAGAGATGGCCCCATCACCATCAGCGACAGCAGCGACGAAGAAAGAATCCCCATGCTGGTCACCCCTGCTCCTCAGCAGCACGAGGAAGAAGATCTGGACGACGACGTGATCCTGACCGAGACAAACAAGCCCCAGCGGAGCAGACCCAACCTGATTAAGCCTGCTGCTCAGTGGCAGGACCTGAAGAGACTGGGCGAAGAGAGGCCCAAGAAGTCCAGAGCCGCCTTCGAGAGCGACAAGAGCAGCTACTTCAGCGTGTGCAACAACCCTCTGTTCGACTCTGGCGCCCAGGACGACAGCGAGGATGATTACGGCGAGTTCCTGGATCTGGGCCCTCCTGGCATCAGCGAGTTTACAAAGCCTAGCGGCCAGACCGAGAGAGAGCCTAAGCCTGGACCTAGCCATAACCAGGCCGCCAACGACATCGTGAACCCCAGATCCGAGCAGAAAGTGATCATCCTGGAAGAAGGCAGCCTGCTGTATACCGAGAGCGATCCCCTGGAAACCCAGAACCAGAGCAGCGAGGACAGCGAGACAGAGCTGCTGAGCAATCTGGGAGAGTCTGCCGCTCTGGCCGATGACCAGGCCATTGAGGAAGATTGCTGGCTGGATCACCCTTACTTCCAGAGCCTGAACCAGCAGCCTCGCGAGATCACCAATCAGGTGGTGCCCCAAGAGAGACAGCCTGAGGCTGAACTGGGCAGACTGCTGTTCCAGCACGAGTTCCCCGGACCTGCCTTTCCTAGACCTGAACCTCAGCAAGGCGGCATCTCTGGCCCATCTTCTCCCCAACCTGCTCACCCTCTGGGCGAGTTTGAAGATCAGCAGCTGGCCTCCGACGACGAGGAACCTGGACCTGCATTCCCCATGCAAGAGAGCCAAGAGCCAAACCTGGAAAACATCTGGGGACAAGAGGCCGCCGAGGTGGACCAAGAACTGGTGGAACTGCTGGTCAAAGAGACAGAGGCCAGATTTCCCGACGTGGCCAACGGCTTCATCGAGGAAATCATCCACTTCAAGAACTACTACGACCTGAACGTGCTGTGCAACTTCCTGCTGGAAAACCCTGACTACCCTAAGAGAGAGGACCGGATCATCATCAACCCCAGCTCTAGCCTGCTGGCCTCTCAGGACGAGACTAAGCTGCCCAAGATCGACTTCTTCGACTACAGCAAGCTGACCCCTCTGGACCAGCGGTGCTTTATCCAGGCAGCCGATCTGCTGATGGCCGACTTCAAGGTGCTGTCCAGCCAGGACATTAAGTGGGCCCTGCACGAGCTGAAGGGCCACTACGCCATTACCAGAAAGGCCCTGAGCGACGCCATCAAGAAGTGGCAAGAGCTGAGCCCTGAGACAAGCGGCAAGCGGAAGAAACGGAAGCAGATGAACCAGTACAGCTACATCGATTTCAAGTTCGAGCAGGGCGACATCAAGATCGAGAAGCGGATGTTCTTTTTGGAGAACAAGCGGCGGCACTGCAGAAGCTACGACAGAAGGGCTCTGCTGCCTGCCGTGCAGCAAGAGCAAGAGTTCTATGAGCAGAAAATCAAAGAAATGGCCGAACACGAGGACTTTTTGCTGGCCCTGCAGATGAATGAGGAACAGTACCAGAAGGACGGCCAGCTGATCGAGTGCAGATGTTGCTACGGGGAGTTCCCATTCGAGGAACTGACCCAGTGTGCCGACGCTCACCTGTTCTGCAAAGAGTGCCTGATCAGATACGCCCAAGAGGCTGTGTTCGGCTCCGGCAAGCTGGAACTGAGCTGTATGGAAGGCAGCTGCACCTGTAGCTTCCCCACCAGCGAGCTGGAAAAGGTGCTGCCTCAGACCATCCTGTACAAGTACTACGAGCGGAAGGCCGAGGAAGAGGTGGCCGCTGCTTATGCCGATGAGCTTGTGCGGTGTCCCTCCTGCTCTTTCCCTGCACTGCTGGATAGCGACGTGAAGAGATTCAGCTGCCCCAATCCTCACTGCCGGAAAGAAACCTGCCGGAAGTGTCAAGGCCTGTGGAAAGAACACAACGGCCTGACCTGCGAAGAACTGGCCGAGAAGGACGATATCAAGTACCGGACCAGCATCGAAGAGAAGATGACCGCCGCCAGAATCCGGAAGTGCCACAAGTGTGGAACCGGCCTGATCAAGAGCGAGGGCTGCAACAGAATGAGCTGCAGATGCGGAGCCCAGATGTGCTACCTGTGCCGGGTGTCCATCAACGGCTACGACCACTTCTGTCAGCACCCTAGAAGTCCCGGCGCTCCCTGTCAAGAGTGCAGCAGATGTAGCCTGTGGACAGACCCCACCGAGGACGACGAGAAGCTGATTGAGGAAATCCAGAAAGAGGCCGAAGAGGAACAGAAGCGCAAGAACGGCGAGAACACCTTCAAGCGGATCGGCGCTGCACTGGAAAAGCCCGTGGAAAAAGTGCAGAGAGTGGAAGCCCTGCCTCGGCCTGTTCCACAGAATCTGCCTCAGCCACAGATGCCTCCATACGCCTTTGCTCACCCACCATTTCCGCTGCCTCCAGTGCGGCCCGTGTTCAACAATTTCCCTCTGAACATGGGCCCCATTCCAGCTCCATACGTGCCACCTCTGCCTAACGTGCGCGTGAACTACGACTTCGGCCCCATCCACATGCCTCTGGAACACAACCTGCCTATGCACTTCGGACCCCAGCCTCGGCACAGATTCTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMEEGNNNEEVIHLNNFHCHRGQEWINLRDGPITISDSSDEERIPMLVTPAPQQHEEEDLDDDVILTETNKPQRSRPNLIKPAAQWQDLKRLGEERPKKSRAAFESDKSSYFSVCNNPLFDSGAQDDSEDDYGEFLDLGPPGISEFTKPSGQTEREPKPGPSHNQAANDIVNPRSEQKVIILEEGSLLYTESDPLETQNQSSEDSETELLSNLGESAALADDQAIEEDCWLDHPYFQSLNQQPREITNQVVPQERQPEAELGRLLFQHEFPGPAFPRPEPQQGGISGPSSPQPAHPLGEFEDQQLASDDEEPGPAFPMQESQEPNLENIWGQEAAEVDQELVELLVKETEARFPDVANGFIEEIIHFKNYYDLNVLCNFLLENPDYPKREDRIIINPSSSLLASQDETKLPKIDFFDYSKLTPLDQRCFIQAADLLMADFKVLSSQDIKWALHELKGHYAITRKALSDAIKKWQELSPETSGKRKKRKQMNQYSYIDFKFEQGDIKIEKRMFFLENKRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTSIEEKMTAARIRKCHKCGTGLIKSEGCNRMSCRCGAQMCYLCRVSINGYDHFCQHPRSPGAPCQECSRCSLWTDPTEDDEKLIEEIQKEAEEEQKRKNGENTFKRIGAALEKPVEKVQRVEALPRPVPQNLPQPQMPPYAFAHPPFPLPPVRPVFNNFPLNMGPIPAPYVPPLPNVRVNYDFGPIHMPLEHNLPMHFGPQPRHRF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU27055
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Codon optimised sequence
• Low Temp: No