cDNA Clone EIF4G1

• Synonyms: eukaryotic translation initiation factor 4 gamma 1 tv7
• Plasmid: pSc-B-EIF4G1 tv 7
• Parent Plasmid: pSC-B
• Genbank: NM_001194947.1 → NP_001181876.1
• Species: Human

Sequence of Insert

ctcgagATGAACAAAGCTCCACAGTCCACAGGCCCCCCACCCGCCCCATC
CCCCGGACTCCCACAGCCAGCGTTTCCCCCGGGGCAGACAGCGCCGGTGG
TGTTCAGTACGCCACAAGCGACACAAATGAACACGCCTTCTCAGCCCCGC
CAGGGAGGATTCAGGTCTCTGCAGCACTTCTACCCTAGCCGGGCCCAGCC
CCCGAGCAGTGCAGCCTCCCGAGTGCAGAGTGCAGCCCCTGCCCGCCCTG
GCCCAGCTGCCCATGTCTACCCTGCTGGATCCCAAGTAATGATGATCCCT
TCCCAGATCTCCTACCCAGCCTCCCAGGGGGCCTACTACATCCCTGGACA
GGGGCGTTCCACATACGTTGTCCCGACACAGCAGTACCCTGTGCAGCCAG
GAGCCCCAGGCTTCTATCCAGGTGCAAGCCCTACAGAATTTGGGACCTAC
GCTGGCGCCTACTATCCAGCCCAAGGGGTGCAGCAGTTTCCCACTGGCGT
GGCCCCCGCCCCAGTTTTGATGAACCAGCCACCCCAGATTGCTCCCAAGA
GGGAGCGTAAGACGATCCGAATTCGAGATCCAAACCAAGGAGGAAAGGAT
ATCACAGAGGAGATCATGTCTGGGGCCCGCACTGCCTCCACACCCACCCC
TCCCCAGACGGGAGGCGGTCTGGAGCCTCAAGCTAATGGGGAGACGCCCC
AGGTTGCTGTCATTGTCCGGCCAGATGACCGGTCACAGGGAGCAATCATT
GCTGACCGGCCAGGGCTGCCTGGCCCAGAGCATAGCCCTTCAGAATCCCA
GCCTTCGTCGCCTTCTCCGACCCCATCACCATCCCCAGTCTTGGAACCGG
GGTCTGAGCCTAATCTCGCAGTCCTCTCTATTCCTGGGGACACTATGACA
ACTATACAAATGTCTGTAGAAGAATCAACCCCCATCTCCCGTGAAACTGG
GGAGCCATATCGCCTCTCTCCAGAACCCACTCCTCTCGCCGAACCCATAC
TGGAAGTAGAAGTGACACTTAGCAAACCGGTTCCAGAATCTGAGTTTTCT
TCCAGTCCTCTCCAGGCTCCCACCCCTTTGGCATCTCACACAGTGGAAAT
TCATGAGCCTAATGGCATGGTCCCATCTGAAGATCTGGAACCAGAGGTGG
AGTCAAGCCCAGAGCTTGCTCCTCCCCCAGCTTGCCCCTCCGAATCCCCT
GTGCCCATTGCTCCAACTGCCCAACCTGAGGAACTGCTCAACGGAGCCCC
CTCGCCACCAGCTGTGGACTTAAGCCCAGTCAGTGAGCCAGAGGAGCAGG
CCAAGGAGGTGACAGCATCAATGGCGCCCCCCACCATCCCCTCTGCTACT
CCAGCTACGGCTCCTTCAGCTACTTCCCCAGCTCAGGAGGAGGAAATGGA
AGAAGAAGAAGAAGAGGAAGAAGGAGAAGCAGGAGAAGCAGGAGAAGCTG
AGAGTGAGAAAGGAGGAGAGGAACTGCTCCCCCCAGAGAGTACCCCTATT
CCAGCCAACTTGTCTCAGAATTTGGAGGCAGCAGCAGCCACTCAAGTGGC
AGTATCTGTGCCAAAGAGGAGACGGAAAATTAAGGAGCTAAATAAGAAGG
AGGCTGTTGGAGACCTTCTGGATGCCTTCAAGGAGGCGAACCCGGCAGTA
CCAGAGGTGGAAAATCAGCCTCCTGCAGGCAGCAATCCAGGCCCAGAGTC
TGAGGGCAGTGGTGTGCCCCCACGTCCTGAGGAAGCAGATGAGACCTGGG
ACTCAAAGGAAGACAAAATTCACAATGCTGAGAACATCCAGCCCGGGGAA
CAGAAGTATGAATATAAGTCAGATCAGTGGAAGCCTCTAAACCTAGAGGA
GAAAAAACGTTACGACCGTGAGTTCCTGCTTGGTTTTCAGTTCATCTTTG
CCAGTATGCAGAAGCCAGAGGGATTGCCACATATCAGTGACGTGGTGCTG
GACAAGGCCAATAAAACACCACTGCGGCCACTGGATCCCACTAGACTACA
AGGCATAAATTGTGGCCCAGACTTCACTCCATCCTTTGCCAACCTTGGCC
GGACAACCCTTAGCACCCGTGGGCCCCCAAGGGGTGGGCCAGGTGGGGAG
CTGCCCCGTGGGCCGGCTGGCCTGGGACCCCGGCGCTCTCAGCAGGGACC
CCGAAAAGAACCACGCAAGATCATTGCCACAGTGTTAATGACCGAAGATA
TAAAACTGAACAAAGCAGAGAAAGCCTGGAAACCCAGCAGCAAGCGGACG
GCGGCTGATAAGGATCGAGGGGAAGAAGATGCTGATGGCAGCAAAACCCA
GGACCTATTCCGCAGGGTGCGCTCCATCCTGAATAAACTGACACCCCAGA
TGTTCCAGCAGCTGATGAAGCAAGTGACGCAGCTGGCCATCGACACCGAG
GAACGCCTCAAAGGGGTCATTGACCTCATTTTTGAGAAGGCCATTTCAGA
GCCCAACTTCTCTGTGGCCTATGCCAACATGTGCCGCTGCCTCATGGCGC
TGAAAGTGCCCACTACGGAAAAGCCAACAGTGACTGTGAACTTCCGAAAG
CTGTTGTTGAATCGATGTCAGAAGGAGTTTGAGAAAGACAAAGATGATGA
TGAGGTTTTTGAGAAGAAGCAAAAAGAGATGGATGAAGCTGCTACGGCAG
AGGAACGAGGACGCCTGAAGGAAGAGCTGGAAGAGGCTCGGGACATAGCC
CGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAGTTGTTCAAACT
GAAGATGTTAACAGAGGCAATAATGCATGACTGTGTGGTCAAACTGCTTA
AGAACCATGATGAAGAGTCCCTTGAGTGCCTTTGTCGTCTGCTCACCACC
ATTGGCAAAGACCTGGACTTTGAAAAAGCCAAGCCCCGAATGGATCAGTA
TTTCAACCAGATGGAAAAAATCATTAAAGAAAAGAAGACGTCATCCCGCA
TCCGCTTTATGCTGCAGGACGTGCTGGATCTGCGAGGGAGCAATTGGGTG
CCACGCCGAGGGGATCAGGGTCCCAAGACCATTGACCAGATCCATAAGGA
GGCTGAGATGGAAGAACATCGAGAGCACATCAAAGTGCAGCAGCTCATGG
CCAAGGGCAGTGACAAGCGTCGGGGCGGTCCTCCAGGCCCTCCCATCAGC
CGTGGACTTCCCCTTGTGGATGATGGTGGCTGGAACACAGTTCCCATCAG
CAAAGGTAGCCGCCCCATTGACACCTCACGACTCACCAAGATCACCAAGC
CTGGCTCCATCGATTCTAACAACCAGCTCTTTGCACCTGGAGGGCGACTG
AGCTGGGGCAAGGGCAGCAGCGGAGGCTCAGGAGCCAAGCCCTCAGACGC
AGCATCAGAAGCTGCTCGCCCAGCTACTAGTACTTTGAATCGCTTCTCAG
CCCTTCAACAAGCGGTACCCACAGAAAGCACAGATAATAGACGTGTGGTG
CAGAGGAGTAGCTTGAGCCGAGAACGAGGCGAGAAAGCTGGAGACCGAGG
AGACCGCCTAGAGCGGAGTGAACGGGGAGGGGACCGTGGGGACCGGCTTG
ATCGTGCGCGGACACCTGCTACCAAGCGGAGCTTCAGCAAGGAAGTGGAG
GAGCGGAGTAGAGAACGGCCCTCCCAGCCTGAGGGGCTGCGCAAGGCAGC
TAGCCTCACGGAGGATCGGGACCGTGGGCGGGATGCCGTGAAGCGAGAAG
CTGCCCTACCCCCAGTGAGCCCCCTGAAGGCGGCTCTCTCTGAGGAGGAG
TTAGAGAAGAAATCCAAGGCTATCATTGAGGAATATCTCCATCTCAATGA
CATGAAAGAGGCAGTCCAGTGCGTGCAGGAGCTGGCCTCACCCTCCTTGC
TCTTCATCTTTGTACGGCATGGTGTCGAGTCTACGCTGGAGCGCAGTGCC
ATTGCTCGTGAGCATATGGGGCAGCTGCTGCACCAGCTGCTCTGTGCTGG
GCATCTGTCTACTGCTCAGTACTACCAAGGGTTGTATGAAATCTTGGAAT
TGGCTGAGGACATGGAAATTGACATCCCCCACGTGTGGCTCTACCTAGCG
GAACTGGTAACACCCATTCTGCAGGAAGGTGGGGTGCCCATGGGGGAGCT
GTTCAGGGAGATTACAAAGCCTCTGAGACCGTTGGGCAAAGCTGCTTCCC
TGTTGCTGGAGATCCTGGGCCTCCTGTGCAAAAGCATGGGTCCTAAAAAG
GTGGGGACGCTGTGGCGAGAAGCCGGGCTTAGCTGGAAGGAATTTCTACC
TGAAGGCCAGGACATTGGTGCATTCGTCGCTGAACAGAAGGTGGAGTATA
CCCTGGGAGAGGAGTCGGAAGCCCCTGGCCAGAGGGCACTCCCCTCCGAG
GAGCTGAACAGGCAGCTGGAGAAGCTGCTGAAGGAGGGCAGCAGTAACCA
GCGGGTGTTCGACTGGATAGAGGCCAACCTGAGTGAGCAGCAGATAGTAT
CCAACACGTTAGTTCGAGCCCTCATGACGGCTGTCTGCTATTCTGCAATT
ATTTTTGAGACTCCCCTCCGAGTGGACGTTGCAGTGCTGAAAGCGCGAGC
GAAGCTGCTGCAGAAATACCTGTGTGACGAGCAGAAGGAGCTACAGGCGC
TCTACGCCCTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTCCCAACCTG
CTGCGGATGTTCTTTGACGCACTGTATGACGAGGACGTGGTGAAGGAGGA
TGCCTTCTACAGTTGGGAGAGTAGCAAGGACCCCGCTGAGCAGCAGGGCA
AGGGTGTGGCCCTTAAATCTGTCACAGCCTTCTTCAAGTGGCTCCGTGAA
GCAGAGGAGGAGTCTGACCACAACTGAgcggccgc

Amino Acid Sequence

MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQG
GFRSLQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQ
ISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAG
AYYPAQGVQQFPTGVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDIT
EEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIAD
RPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTI
QMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSS
PLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVP
IAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPA
TAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPA
NLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPE
VENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQK
YEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDK
ANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELP
RGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAA
DKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEER
LKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLL
LNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARR
RSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIG
KDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPR
RGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRG
LPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSW
GKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQR
SSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEER
SRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELE
KKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIA
REHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAEL
VTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVG
TLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEEL
NRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIF
ETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLR
MFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAE
EESDHN*

• Vector Type: Mammalian
• Antibiotic: Amp; Kan
• DU Number: DU28502
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: transcript variant 7. Compared to tv2 (isoform 5) this clone has a 7 residue insertion at position 45. in isoform 5 and tv7 Q697 is deleted (compared with isoform 1)