cDNA Clone Fam83D

• Plasmid: pcDNA5D FRT/TO-FAM83D
• Parent Plasmid: pcDNA5D FRT/TO
• Expressed: Fam83D
• Genbank: NM_030919.2
• Species: Human

Sequence of Insert

ggatccATGGCGCGGGCCTGTTTGATTCAAAGGTTGCCTATAAAGCGGGA
CTGCACGCCGGTTTTTGTCCGAGGGCTGTCGAGTCCGAGCGCCGCCATGG
CTCTGCTGTCCGAGGGCCTGGACGAGGTGCCCGCCGCCTGCCTGTCGCCG
TGCGGGCCGCCCAACCCGACCGAGCTGTTCAGCGAGTCACGGCGCCTGGC
TCTGGAGGAGCTGGTGGCGGGCGGCCCCGAAGCCTTCGCGGCCTTCCTGC
GACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATGAGGTGCACGCCATT
CTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGGCGGCGGCGGC
GGCCGAGGACTCGTTCGGCTCCTCGCACGACTGCTCTTCGGGCACCTACT
TCCCCGAGCAGTCGGACCTGGAGCCACCGCTGTTGGAGCTTGGCTGGCCC
GCCTTCTACCAGGGCGCCTACCGCGGCGCCACGCGTGTCGAGACGCACTT
CCAGCCCCGCGGCGCTGGCGAAGGTGGCCCCTACGGCTGCAAGGACGCTC
TGCGCCAGCAGCTCCGCTCGGCGCGAGAGGTGATTGCAGTGGTCATGGAC
GTGTTCACAGACATCGACATCTTCAGAGACCTGCAAGAAATATGCAGGAA
ACAGGGAGTTGCTGTGTATATCCTTCTGGACCAGGCTCTCCTCTCTCAAT
TTCTGGATATGTGCATGGATCTGAAAGTTCATCCTGAACAGGAAAAGTTA
ATGACAGTTCGGACTATCACAGGAAATATCTACTATGCAAGGTCAGGAAC
TAAGATTATTGGGAAGGTTCACGAAAAGTTCACGTTGATTGATGGCATCC
GCGTGGCAACAGGCTCCTACAGTTTTACATGGACGGATGGCAAATTAAAC
AGCAGTAACTTGGTAATTCTGTCTGGCCAAGTGGTTGAACACTTTGATCT
GGAATTCCGAATCCTGTATGCCCAGTCCAAGCCCATCAGCCCCAAACTCC
TGTCTCACTTCCAGAGCAGCAACAAGTTTGATCACCTCACCAACCGAAAA
CCACAGTCCAAGGAGCTCACCCTGGGCAACCTGCTGCGGATGCGGCTGGC
TAGGCTGTCAAGTACTCCCAGGAAGGCGGACCTGGACCCAGAGATGCCCG
CAGAGGGCAAGGCAGAGCGCAAGCCCCATGACTGTGAGTCCTCTACTGTT
AGTGAGGAAGACTACTTCAGCAGCCACAGGGACGAGCTCCAGAGCAGAAA
GGCCATTGACGCTGCCACTCAAACAGAGCCAGGAGAGGAGATGCCAGGGC
TGAGTGTGAGTGAGGTGGGAACACAAACCAGCATCACCACAGCATGTGCT
GGTACCCAGACTGCAGTCATCACCAGGATAGCAAGCTCTCAAACCACGAT
TTGGTCCAGATCGACCACTACTCAGACTGACATGGATGAGAACATTCTCT
TTCCTCGAGGAACTCAATCTACAGAAGGGTCACCAGTCTCAAAAATGTCT
GTATCGAGATCTTCCAGTTTGAAGTCTTCCTCCTCTGTGTCTTCCCAAGG
CTCTGTGGCAAGCTCCACTGGTTCTCCCGCTTCCATCAGAACCACTGACT
TCCACAATCCTGGCTATCCCAAGTACCTGGGCACCCCCCACCTGGAACTG
TACTTGAGTGACTCACTTAGAAACTTGAACAAAGAGCGGCAATTCCACTT
CGCTGGTATCAGGTCCCGGCTCAACCACATGCTGGCTATGCTGTCAAGGA
GAACACTCTTTACTGAAAACCACCTTGGCCTTCATTCTGGCAATTTCAGC
AGAGTTAATTTGCTTGCTGTTAGAGATGTAGCACTTTATCCTTCCTATCA
GTAAgtcgacTCGAGCGGCCG

Amino Acid Sequence

MARACLIQRLPIKRDCTPVFVRGLSSPSAAMALLSEGLDEVPAACLSPCG
PPNPTELFSESRRLALEELVAGGPEAFAAFLRRERLARFLNPDEVHAILR
AAERPGEEGAAAAAAAEDSFGSSHDCSSGTYFPEQSDLEPPLLELGWPAF
YQGAYRGATRVETHFQPRGAGEGGPYGCKDALRQQLRSAREVIAVVMDVF
TDIDIFRDLQEICRKQGVAVYILLDQALLSQFLDMCMDLKVHPEQEKLMT
VRTITGNIYYARSGTKIIGKVHEKFTLIDGIRVATGSYSFTWTDGKLNSS
NLVILSGQVVEHFDLEFRILYAQSKPISPKLLSHFQSSNKFDHLTNRKPQ
SKELTLGNLLRMRLARLSSTPRKADLDPEMPAEGKAERKPHDCESSTVSE
EDYFSSHRDELQSRKAIDAATQTEPGEEMPGLSVSEVGTQTSITTACAGT
QTAVITRIASSQTTIWSRSTTTQTDMDENILFPRGTQSTEGSPVSKMSVS
RSSSLKSSSSVSSQGSVASSTGSPASIRTTDFHNPGYPKYLGTPHLELYL
SDSLRNLNKERQFHFAGIRSRLNHMLAMLSRRTLFTENHLGLHSGNFSRV
NLLAVRDVALYPSYQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28503
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent GAG > GAA at Glu316. can be excised Bam-Sal1 or Bam-Not1