cDNA Clone EIF4G1

• Synonyms: eukaryotic translation initiation factor 4 gamma 1 tv7
• Plasmid: pSc-B-EIF4G1 tv 2
• Parent Plasmid: pSC-B
• Genbank: NM_001194947.1 → NP_001181876.1
• Species: Human

Sequence of Insert

CTCGAGATGAACAAAGCTCCACAGTCCACAGGCCCCCCACCCGCCCCATCCCCCGGACTCCCACAGCCAGCGTTTCCCCCGGGGCAGACAGCGCCGGTGG
TGTTCAGTACGCCACAAGCGACACAAATGAACACGCCTTCTCAGCCCCGCCAGCACTTCTACCCTAGCCGGGCCCAGCCCCCGAGCAGTGCAGCCTCCCG
AGTGCAGAGTGCAGCCCCTGCCCGCCCTGGCCCAGCTGCCCATGTCTACCCTGCTGGATCCCAAGTAATGATGATCCCTTCCCAGATCTCCTACCCAGCC
TCCCAGGGGGCCTACTACATCCCTGGACAGGGGCGTTCCACATACGTTGTCCCGACACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAG
GTGCAAGCCCTACAGAATTTGGGACCTACGCTGGCGCCTACTATCCAGCCCAAGGGGTGCAGCAGTTTCCCACTGGCGTGGCCCCCGCCCCAGTTTTGAT
GAACCAGCCACCCCAGATTGCTCCCAAGAGGGAGCGTAAGACGATCCGAATTCGAGATCCAAACCAAGGAGGAAAGGATATCACAGAGGAGATCATGTCT
GGGGCCCGCACTGCCTCCACACCCACCCCTCCCCAGACGGGAGGCGGTCTGGAGCCTCAAGCTAATGGGGAGACGCCCCAGGTTGCTGTCATTGTCCGGC
CAGATGACCGGTCACAGGGAGCAATCATTGCTGACCGGCCAGGGCTGCCTGGCCCAGAGCATAGCCCTTCAGAATCCCAGCCTTCGTCGCCTTCTCCGAC
CCCATCACCATCCCCAGTCTTGGAACCGGGGTCTGAGCCTAATCTCGCAGTCCTCTCTATTCCTGGGGACACTATGACAACTATACAAATGTCTGTAGAA
GAATCAACCCCCATCTCCCGTGAAACTGGGGAGCCATATCGCCTCTCTCCAGAACCCACTCCTCTCGCCGAACCCATACTGGAAGTAGAAGTGACACTTA
GCAAACCGGTTCCAGAATCTGAGTTTTCTTCCAGTCCTCTCCAGGCTCCCACCCCTTTGGCATCTCACACAGTGGAAATTCATGAGCCTAATGGCATGGT
CCCATCTGAAGATCTGGAACCAGAGGTGGAGTCAAGCCCAGAGCTTGCTCCTCCCCCAGCTTGCCCCTCCGAATCCCCTGTGCCCATTGCTCCAACTGCC
CAACCTGAGGAACTGCTCAACGGAGCCCCCTCGCCACCAGCTGTGGACTTAAGCCCAGTCAGTGAGCCAGAGGAGCAGGCCAAGGAGGTGACAGCATCAA
TGGCGCCCCCCACCATCCCCTCTGCTACTCCAGCTACGGCTCCTTCAGCTACTTCCCCAGCTCAGGAGGAGGAAATGGAAGAAGAAGAAGAAGAGGAAGA
AGGAGAAGCAGGAGAAGCAGGAGAAGCTGAGAGTGAGAAAGGAGGAGAGGAACTGCTCCCCCCAGAGAGTACCCCTATTCCAGCCAACTTGTCTCAGAAT
TTGGAGGCAGCAGCAGCCACTCAAGTGGCAGTATCTGTGCCAAAGAGGAGACGGAAAATTAAGGAGCTAAATAAGAAGGAGGCTGTTGGAGACCTTCTGG
ATGCCTTCAAGGAGGCGAACCCGGCAGTACCAGAGGTGGAAAATCAGCCTCCTGCAGGCAGCAATCCAGGCCCAGAGTCTGAGGGCAGTGGTGTGCCCCC
ACGTCCTGAGGAAGCAGATGAGACCTGGGACTCAAAGGAAGACAAAATTCACAATGCTGAGAACATCCAGCCCGGGGAACAGAAGTATGAATATAAGTCA
GATCAGTGGAAGCCTCTAAACCTAGAGGAGAAAAAACGTTACGACCGTGAGTTCCTGCTTGGTTTTCAGTTCATCTTTGCCAGTATGCAGAAGCCAGAGG
GATTGCCACATATCAGTGACGTGGTGCTGGACAAGGCCAATAAAACACCACTGCGGCCACTGGATCCCACTAGACTACAAGGCATAAATTGTGGCCCAGA
CTTCACTCCATCCTTTGCCAACCTTGGCCGGACAACCCTTAGCACCCGTGGGCCCCCAAGGGGTGGGCCAGGTGGGGAGCTGCCCCGTGGGCCGGCTGGC
CTGGGACCCCGGCGCTCTCAGCAGGGACCCCGAAAAGAACCACGCAAGATCATTGCCACAGTGTTAATGACCGAAGATATAAAACTGAACAAAGCAGAGA
AAGCCTGGAAACCCAGCAGCAAGCGGACGGCGGCTGATAAGGATCGAGGGGAAGAAGATGCTGATGGCAGCAAAACCCAGGACCTATTCCGCAGGGTGCG
CTCCATCCTGAATAAACTGACACCCCAGATGTTCCAGCAGCTGATGAAGCAAGTGACGCAGCTGGCCATCGACACCGAGGAACGCCTCAAAGGGGTCATT
GACCTCATTTTTGAGAAGGCCATTTCAGAGCCCAACTTCTCTGTGGCCTATGCCAACATGTGCCGCTGCCTCATGGCGCTGAAAGTGCCCACTACGGAAA
AGCCAACAGTGACTGTGAACTTCCGAAAGCTGTTGTTGAATCGATGTCAGAAGGAGTTTGAGAAAGACAAAGATGATGATGAGGTTTTTGAGAAGAAGCA
AAAAGAGATGGATGAAGCTGCTACGGCAGAGGAACGAGGACGCCTGAAGGAAGAGCTGGAAGAGGCTCGGGACATAGCCCGGCGGCGCTCTTTAGGGAAT
ATCAAGTTTATTGGAGAGTTGTTCAAACTGAAGATGTTAACAGAGGCAATAATGCATGACTGTGTGGTCAAACTGCTTAAGAACCATGATGAAGAGTCCC
TTGAGTGCCTTTGTCGTCTGCTCACCACCATTGGCAAAGACCTGGACTTTGAAAAAGCCAAGCCCCGAATGGATCAGTATTTCAACCAGATGGAAAAAAT
CATTAAAGAAAAGAAGACGTCATCCCGCATCCGCTTTATGCTGCAGGACGTGCTGGATCTGCGAGGGAGCAATTGGGTGCCACGCCGAGGGGATCAGGGT
CCCAAGACCATTGACCAGATCCATAAGGAGGCTGAGATGGAAGAACATCGAGAGCACATCAAAGTGCAGCAGCTCATGGCCAAGGGCAGTGACAAGCGTC
GGGGCGGTCCTCCAGGCCCTCCCATCAGCCGTGGACTTCCCCTTGTGGATGATGGTGGCTGGAACACAGTTCCCATCAGCAAAGGTAGCCGCCCCATTGA
CACCTCACGACTCACCAAGATCACCAAGCCTGGCTCCATCGATTCTAACAACCAGCTCTTTGCACCTGGAGGGCGACTGAGCTGGGGCAAGGGCAGCAGC
GGAGGCTCAGGAGCCAAGCCCTCAGACGCAGCATCAGAAGCTGCTCGCCCAGCTACTAGTACTTTGAATCGCTTCTCAGCCCTTCAACAAGCGGTACCCA
CAGAAAGCACAGATAATAGACGTGTGGTGCAGAGGAGTAGCTTGAGCCGAGAACGAGGCGAGAAAGCTGGAGACCGAGGAGACCGCCTAGAGCGGAGTGA
ACGGGGAGGGGACCGTGGGGACCGGCTTGATCGTGCGCGGACACCTGCTACCAAGCGGAGCTTCAGCAAGGAAGTGGAGGAGCGGAGTAGAGAACGGCCC
TCCCAGCCTGAGGGGCTGCGCAAGGCAGCTAGCCTCACGGAGGATCGGGACCGTGGGCGGGATGCCGTGAAGCGAGAAGCTGCCCTACCCCCAGTGAGCC
CCCTGAAGGCGGCTCTCTCTGAGGAGGAGTTAGAGAAGAAATCCAAGGCTATCATTGAGGAATATCTCCATCTCAATGACATGAAAGAGGCAGTCCAGTG
CGTGCAGGAGCTGGCCTCACCCTCCTTGCTCTTCATCTTTGTACGGCATGGTGTCGAGTCTACGCTGGAGCGCAGTGCCATTGCTCGTGAGCATATGGGG
CAGCTGCTGCACCAGCTGCTCTGTGCTGGGCATCTGTCTACTGCTCAGTACTACCAAGGGTTGTATGAAATCTTGGAATTGGCTGAGGACATGGAAATTG
ACATCCCCCACGTGTGGCTCTACCTAGCGGAACTGGTAACACCCATTCTGCAGGAAGGTGGGGTGCCCATGGGGGAGCTGTTCAGGGAGATTACAAAGCC
TCTGAGACCGTTGGGCAAAGCTGCTTCCCTGTTGCTGGAGATCCTGGGCCTCCTGTGCAAAAGCATGGGTCCTAAAAAGGTGGGGACGCTGTGGCGAGAA
GCCGGGCTTAGCTGGAAGGAATTTCTACCTGAAGGCCAGGACATTGGTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCGGAAG
CCCCTGGCCAGAGGGCACTCCCCTCCGAGGAGCTGAACAGGCAGCTGGAGAAGCTGCTGAAGGAGGGCAGCAGTAACCAGCGGGTGTTCGACTGGATAGA
GGCCAACCTGAGTGAGCAGCAGATAGTATCCAACACGTTAGTTCGAGCCCTCATGACGGCTGTCTGCTATTCTGCAATTATTTTTGAGACTCCCCTCCGA
GTGGACGTTGCAGTGCTGAAAGCGCGAGCGAAGCTGCTGCAGAAATACCTGTGTGACGAGCAGAAGGAGCTACAGGCGCTCTACGCCCTCCAGGCCCTTG
TAGTGACCTTAGAACAGCCTCCCAACCTGCTGCGGATGTTCTTTGACGCACTGTATGACGAGGACGTGGTGAAGGAGGATGCCTTCTACAGTTGGGAGAG
TAGCAAGGACCCCGCTGAGCAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCCTTCTTCAAGTGGCTCCGTGAAGCAGAGGAGGAGTCTGACCAC
AACTGAGCGGCCGC

Amino Acid Sequence

MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQH
FYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQ
GAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQG
VQQFPTGVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGA
RTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGP
EHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEES
TPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTP
LASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQP
EELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATS
PAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLE
AAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPA
GSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQ
WKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLR
PLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLG
PRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEE
DADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDL
IFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKE
FEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIK
FIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEK
AKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPK
TIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDG
GWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGG
SGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRER
GEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQ
PEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAII
EEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQL
LHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQE
GGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAG
LSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKL
LKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVD
VAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALY
DEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN*

• Vector Type: Mammalian
• Antibiotic: Amp; Kan
• DU Number: DU28517
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: tv2 (isoform 5). created by deletion of 7 residue insertion at position 45 in tv7. in isoform 5 and tv7 Q697 is deleted (compared with isoform 1). clone contains Thr161Ala SNP