cDNA Clone MARK2

• Plasmid: pcDNA5-FRT/TO-mCherry-MARK2
• Parent Plasmid: pcDNA5-FRT/TO-mCherry
• Expressed: mCherry-MARK2
• Genbank: NM_004954
• Tag: mCherry
• Species: Human

Sequence of Insert

agatctATGATTCGGGGCCGCAACTCAGCCACCTCTGCTGATGAGCAGCC
CCACATTGGAAACTACCGGCTCCTCAAGACCATTGGCAAGGGTAATTTTG
CCAAGGTGAAGTTGGCCCGACACATCCTGACTGGGAAAGAGGTAGCTGTG
AAGATCATTGACAAGACTCAACTGAACTCCTCCAGCCTCCAGAAACTATT
CCGCGAAGTAAGAATAATGAAGGTTTTGAATCATCCCAACATAGTTAAAT
TATTTGAAGTGATTGAGACTGAGAAAACGCTCTACCTTGTCATGGAGTAC
GCTAGTGGCGGAGAGGTATTTGATTACCTAGTGGCTCATGGCAGGATGAA
AGAAAAAGAGGCTCGAGCCAAATTCCGCCAGATAGTGTCTGCTGTGCAGT
ACTGTCACCAGAAGTTTATTGTCCATAGAGACTTAAAGGCAGAAAACCTG
CTCTTGGATGCTGATATGAACATCAAGATTGCAGACTTTGGCTTCAGCAA
TGAATTCACCTTTGGGAACAAGCTGGACACCTTCTGTGGCAGTCCCCCTT
ATGCTGCCCCAGAACTCTTCCAGGGCAAAAAATATGATGGACCCGAGGTG
GATGTGTGGAGCCTAGGAGTTATCCTCTATACACTGGTCAGCGGATCCCT
GCCTTTTGATGGACAGAACCTCAAGGAGCTGCGGGAACGGGTACTGAGGG
GAAAATACCGTATTCCATTCTACATGTCCACGGACTGTGAAAACCTGCTT
AAGAAATTTCTCATTCTTAATCCCAGCAAGAGAGGCACTTTAGAGCAAAT
CATGAAAGATCGATGGATGAATGTGGGTCACGAAGATGATGAACTAAAGC
CTTACGTGGAGCCACTCCCTGACTACAAGGACCCCCGGCGGACAGAGCTG
ATGGTGTCCATGGGTTATACACGGGAAGAGATCCAGGACTCGCTGGTGGG
CCAGAGATACAACGAGGTGATGGCCACCTATCTGCTCCTGGGCTACAAGA
GCTCCGAGCTGGAAGGCGACACCATCACCCTGAAACCCCGGCCTTCAGCT
GATCTGACCAATAGCAGCGCCCCATCCCCATCCCACAAGGTACAGCGCAG
CGTGTCGGCCAATCCCAAGCAGCGGCGCTTCAGCGACCAGGCTGGTCCTG
CCATTCCCACCTCTAATTCTTACTCTAAGAAGACTCAGAGTAACAACGCA
GAAAATAAGCGGCCTGAGGAGGACCGGGAGTCAGGGCGGAAAGCCAGCAG
CACAGCCAAGGTGCCTGCCAGCCCCCTGCCCGGTCTGGAGAGGAAGAAGA
CCACCCCAACCCCCTCCACGAACAGCGTCCTCTCCACCAGCACAAATCGA
AGCAGGAATTCCCCACTTTTGGAGCGGGCCAGCCTCGGCCAGGCCTCCAT
CCAGAATGGCAAAGACAGCACAGCCCCCCAGCGTGTCCCTGTTGCCTCCC
CATCCGCCCACAACATCAGCAGCAGTGGTGGAGCCCCAGACCGAACTAAC
TTCCCCCGGGGTGTGTCCAGCCGAAGCACCTTCCATGCTGGGCAGCTCCG
ACAGGTGCGGGACCAGCAGAATTTGCCCTACGGTGTGACCCCAGCCTCTC
CCTCTGGCCACAGCCAGGGCCGGCGGGGGGCCTCTGGGAGCATCTTCAGC
AAGTTCACCTCCAAGTTTGTACGCAGGAACCTGAATGAACCTGAAAGCAA
AGACCGAGTGGAGACGCTCAGACCTCACGTGGTGGGCAGTGGCGGCAACG
ACAAAGAAAAGGAAGAATTTCGGGAGGCCAAGCCCCGCTCCCTCCGCTTC
ACGTGGAGTATGAAGACCACGAGCTCCATGGAGCCCAACGAGATGATGCG
GGAGATCCGCAAGGTGCTGGACGCGAACAGCTGCCAGAGCGAGCTGCATG
AGAAGTACATGCTGCTGTGCATGCACGGCACGCCGGGCCACGAGGACTTC
GTGCAGTGGGAGATGGAGGTGTGCAAACTGCCGCGGCTCTCTCTCAACGG
GGTTCGATTTAAGCGGATATCGGGCACCTCCATGGCCTTCAAAAACATTG
CCTCCAAAATAGCCAACGAGCTGAAGCTTTAAGCGGCCGC

Amino Acid Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWER
VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA
SSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
NIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKSGLGSMIRGRNSAT
SADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSS
SLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLV
AHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIA
DFGFSNEFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYT
LVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKR
GTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVSMGYTREEI
QDSLVGQRYNEVMATYLLLGYKSSELEGDTITLKPRPSADLTNSSAPSPS
HKVQRSVSANPKQRRFSDQAGPAIPTSNSYSKKTQSNNAENKRPEEDRES
GRKASSTAKVPASPLPGLERKKTTPTPSTNSVLSTSTNRSRNSPLLERAS
LGQASIQNGKDSTAPQRVPVASPSAHNISSSGGAPDRTNFPRGVSSRSTF
HAGQLRQVRDQQNLPYGVTPASPSGHSQGRRGASGSIFSKFTSKFVRRNL
NEPESKDRVETLRPHVVGSGGNDKEKEEFREAKPRSLRFTWSMKTTSSME
PNEMMREIRKVLDANSCQSELHEKYMLLCMHGTPGHEDFVQWEMEVCKLP
RLSLNGVRFKRISGTSMAFKNIASKIANELKL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28519
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Changes to NM_004954 ORF sequence: ; g696a (silent in G232); c759t (silent in I253); a1050g (silent in L350)*; a1067c (Q356P)*; t1070c (F357S)*; a1092c (silent in R364)*; * natural variants