cDNA Clone Huntingtin

• Plasmid: pBABE hygro HTT Q25 mCherry
• Parent Plasmid: pBabe hygro C-mCherry
• Expressed: HTT-Q25-mCherry
• Tag: mCherry

Sequence of Insert

AGATCTATGGCGACCCTGGAAAAGCTGATGAAGGCCTTCGAGTCCCTCAAAAGCTTCCAACAGCAGCAACAGCAACAACAGCAGCAACAGCAACAACAGCAGCAACAGCAACAACAGCAGCAACAGCAACAACCGCCACCACCTCCCCCTCCACCCCCACCTCCTCAACTTCCTCAACCTCCTCCACAGGCACAGCCTCTGCTGCCTCAGCCACAACCTCCTCCACCTCCACCTCCACCTCCTCCAGGCCCAGCTGTGGCTGAGGAGCCTCTGCACCGACCTGGATCCCTGGAATTC

Amino Acid Sequence

MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPQLPQPPPQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRPGSLEFGHMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK*

• Vector Type: Mammalian
• DU Number: DU55417
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please clone HTT-Q25 (from DU25503) into pBabe hygro, with mCherry on C-terminus. Thanks!
• Low Temp: No