cDNA Clone YPL191C

• Plasmid: pGEX6P1 YPL191C 1-270 C28A
• Parent Plasmid: pGEX6P-1
• Expressed: GST-YPL191C 1-270 C28A
• Tag: GST

Sequence of Insert

GGATCCCCCGGGCTGCAGCGACCAATGTGGAATTCGCCCTTGGATCTGATGGATTTAAGTTTCACGACTAAAAGTGTCAAAATTAATGGTCAAAACCATCGAATATTACTGCAAAACGAAAACGGACCTGCTGCCTTGTTAGCACTTGCAAATATTCTAATACTTTCACCAGATCATACACGTTTTTCCAATGAGTTAATAAGGTTGGTAAATAAGGGAAGTCAAATATCTTTAAAAGAACTTATTGAAGTCTTAGCAGATATCGCTTTACAAGTTACAGATAAACCTAGTACTGATATAAGTGAGTTACTAAGTTTGTTACCTAGATTACATGAAGGTTTAAATATTAACCCTGAGTTTAATGGGTCTTTTGAAAATACTAAAGAGATGTCAATATTTCGATTGTTTAACGTTGATGTTGTCCATGGCTGGGTTATCAACAGTTTCATAAATGAAAACATTGATGAGAAACTATCTCATTATTCTTATGAATCTGCGCAGAGAATCTTAACACAAGCAGCTGATATAAATTGTGGAATTTCCCAAGATGAAAACTCGGACGAAGTTTTACGTGATGCCATGCATTTGGGTCTGTTTTTGAACGAATCACCGACTCAGTTGACCGCCTTTGGACTCCTACGATTGAGAGAAAAGCTGCTACACAACAAATTCTCCATTTTATTCAGAAATGACCATTTTTCTACATTGTTCAAATACGAAGATCGATTATACACATTGGTTACGGACTTTGGTTATAAAAATTGTAAAGATATTGTATGGCAATCCCTAGATTCGGTGGACGGCTCCTGCGATGCTTTTTTTGCCGGTAACTTTAGTGCAGCAGAGGTAAATGGACAGTAACAATTGTCAACTGACATTGAACGTGATTTTGGAACTGGAAATTTGCTACTTGAAGAAATTCAGCAAATTGAAAACGATAAAGAACTAGCCAAACAGTTACAGGAACAAGAACAAGAACGTGTTACGAAATTTGAAGCAAAAAGGAAAATCCATTCTCACAAAAAAAACTCAGAAATACACGCACCAGTCAAAAAAGACAAGTTTAAGCGAAGAAGCAGCCTTCTCAATGCAAAAGCTTCTGAAAAAGAGAAAAGTGAGTGCGTTGTTATGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGLQRPMWNSPLDLMDLSFTTKSVKINGQNHRILLQNENGPAALLALANILILSPDHTRFSNELIRLVNKGSQISLKELIEVLADIALQVTDKPSTDISELLSLLPRLHEGLNINPEFNGSFENTKEMSIFRLFNVDVVHGWVINSFINENIDEKLSHYSYESAQRILTQAADINCGISQDENSDEVLRDAMHLGLFLNESPTQLTAFGLLRLREKLLHNKFSILFRNDHFSTLFKYEDRLYTLVTDFGYKNCKDIVWQSLDSVDGSCDAFFAGNFSAAEVNGQ*

• Vector Type: Bacterial
• DU Number: DU55338
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: please mutate Cys 28 to Ala in 55257