cDNA Clone FAM83H

• Plasmid: pGEX6P-1-FAM83H D236A
• Parent Plasmid: pGEX6P-1
• Expressed: GST-FAM83H D236A
• Genbank: NM_198488.3
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGCCCGTCGCTCTCAGAGCTCCTCGCAGGGGGACAACCCACTGGCACC
CGGGTACCTGCCGCCTCACTACAAAGAGTACTACCGCCTGGCGGTGGATG
CACTGGCCGAGGGTGGCTCGGAGGCCTACAGCCGCTTCCTCGCTACCGAG
GGGGCACCAGACTTCCTGTGCCCTGAAGAGCTGGAACATGTGAGCCGACA
CCTTCGGCCTCCGCAGTATGTTACCCGAGAGCCACCTGAAGGCAGCCTTC
TCGACGTGGACATGGATGGCTCCTCGGGTACATACTGGCCAGTGAACTCA
GACCAGGCCGTGCCTGAGCTTGATTTGGGCTGGCCTCTGACCTTCGGCTT
CCAGGGCACCGAGGTGACCACCTTGGTGCAGCCACCGCCCCCCGACAGCC
CCAGTATCAAGGATGAGGCCCGCAGGATGATCCGTTCCGCCCAGCAGGTG
GTGGCCGTGGTGATGGACATGTTCACTGATGTGGACCTGCTCAGCGAAGT
GCTGGAGGCCGCGGCCCGTCGGGTCCCAGTCTACATCCTGCTGGATGAGA
TGAACGCGCAGCACTTCCTGGACATGGCCGACAAGTGCCGTGTCAACCTG
CACCACGTGGATTTCCTGCGCGTACGGACTGTGGCGGGCCCCACCTACTA
CTGCCGCACTGGGAAGTCCTTCAAGGGCCACGTCAAGGAGAAGTTCCTGC
TGGTGGCCTGTGCCGTGGTGATGAGTGGGAGCTACAGCTTCATGTGGTCC
TTTGAGAAGATCCACCGCAGCCTGGCGCACGTGTTCCAAGGAGAGCTGGT
CTCCAGCTTCGACGAGGAGTTCCGCATCCTCTTCGCGCAGTCCGAGCCGC
TTGTGCCCTCGGCCGCGGCCCTGGCCCGCATGGACGCCTATGCCCTGGCT
CCGTATGCCGGGGCCGGGCCTCTCGTGGGCGTCCCTGGGGTCGGGGCGCC
AACCCCCTTCTCCTTCCCTAAACGAGCGCACCTCCTGTTCCCGCCACCCC
GGGAAGAGGGCCTGGGCTTCCCCTCCTTCCTCGACCCGGACCGCCACTTC
CTGTCGGCCTTCCGCCGGGAGGAGCCGCCGCGGATGCCGGGGGGCGCGCT
GGAACCGCACGCGGGGCTGCGGCCACTCTCGCGGCGCCTGGAGGCCGAGG
CCGGGCCGGCTGGGGAGCTCGCGGGCGCGCGGGGCTTCTTCCAGGCGCGG
CACCTGGAGATGGACGCCTTCAAGCGGCACAGCTTCGCGACCGAGGGCGC
GGGCGCCGTGGAGAACTTCGCGGCTGCGCGGCAGGTGTCGCGGCAGACGT
TCCTCAGCCACGGCGACGACTTCCGCTTCCAGACCAGCCACTTCCACCGT
GACCAGCTCTACCAGCAGCAGTACCAGTGGGACCCGCAGCTCACGCCGGC
GCGCCCGCAAGGCCTGTTCGAGAAGCTTCGCGGGGGCCGCGCGGGTTTCG
CGGACCCGGATGACTTCACCCTGGGCGCCGGGCCCCGCTTCCCGGAGCTC
GGACCCGACGGGCACCAGCGGCTGGACTACGTGCCGTCCAGCGCGTCCCG
CGAGGTGCGCCACGGCTCGGACCCCGCCTTCGCGCCCGGACCCCGCGGCC
TGGAGCCCAGCGGAGCCCCGCGCCCCAACCTGACCCAGCGCTTCCCATGC
CAGGCCGCGGCGAGGCCGGGCCCAGACCCCGCTCCCGAGGCGGAGCCGGA
GCGCAGGGGCGGGCCCGAGGGGCGGGCAGGGCTGCGGCGCTGGCGTTTGG
CCTCCTACTTGAGCGGCTGCCACGGCGAGGATGGGGGCGACGACGGCCTA
CCGGCGCCCATGGAAGCGGAGGCTTACGAAGACGACGTGCTGGCTCCCGG
GGGCCGGGCACCTGCCGGCGACCTGCTCCCCTCGGCCTTCCGCGTCCCAG
CAGCCTTCCCCACCAAGGTCCCGGTGCCAGGCCCGGGCAGCGGCGGCAAC
GGCCCAGAGCGCGAGGGCCCGGAGGAGCCTGGCCTGGCCAAGCAGGACTC
ATTCCGCTCGCGCCTGAACCCCCTGGTCCAGCGCAGCTCCAGGCTGCGCT
CCTCGCTCATCTTCAGCACGTCACAGGCCGAGGGCGCGGCCGGGGCTGCG
GCGGCCACTGAGAAGGTGCAGCTGCTGCACAAGGAGCAGACGGTCAGCGA
GACGCTGGGGCCCGGCGGAGAGGCCGTGCGCTCCGCGGCTTCCACCAAGG
TGGCGGAGCTGCTGGAGAAGTACAAGGGCCCAGCCCGTGATCCCGGCGGC
GGCGCGGGCGCCATCACCGTTGCCAGCCACAGCAAGGCCGTCGTGTCCCA
GGCGTGGCGGGAAGAGGTGGCGGCCCCAGGTGCCGTGGGGGGCGAGCGCC
GCAGCCTCGAGAGCTGCCTGCTGGACCTGCGCGACTCCTTTGCACAGCAG
CTGCACCAGGAGGCGGAGCGGCAGCCGGGAGCCGCGTCGCTCACCGCGGC
GCAGCTGCTCGACACACTGGGCCGGAGCGGCTCCGACCGCCTGCCTTCCC
GCTTCCTCTCTGCCCAGAGCCACTCAACGTCCCCGCAAGGGCTGGACAGC
CCTCTGCCGCTGGAAGGGTCCGGAGCGCACCAGGTGCTCCATAATGAGTC
AAAAGGGAGCCCCACCTCGGCTTACCCTGAGCGGAAGGGGAGCCCCACGC
CTGGGTTTTCCACTCGAAGAGGAAGTCCAACTACAGGATTTATCGAGCAG
AAGGGGAGCCCCACCTCAGCCTACCCCGAGCGCAGGGGTAGTCCGGTGCC
CCCCGTGCCGGAGCGCAGGAGCAGTCCGGTGCCCCCCGTGCCGGAGCGCA
GGGGCAGCCTCACCCTTACCATCTCCGGGGAGTCCCCGAAGGCCGGGCCC
GCGGAGGAGGGGCCGAGCGGCCCCATGGAAGTCCTGCGCAAAGGCTCCTT
GCGTCTTAGGCAGCTGCTGAGCCCCAAGGGCGAGCGGCGCATGGAGGATG
AGGGTGGCTTCCCAGTGCCGCAGGAGAACGGCCAACCCGAGAGCCCGCGG
CGTCTGTCACTGGGCCAGGGTGACAGCACGGAGGCTGCCACAGAAGAGCG
GGGTCCGCGGGCGCGCCTGTCCTCAGCCACGGCCAACGCCTTGTACAGCA
GCAACCTTCGGGATGACACGAAGGCCATTCTGGAGCAGATCAGTGCCCAC
GGCCAGAAGCACCGTGCGGTCCCTGCCCCGAGCCCCGGCCCGACCCACAA
CAGCCCCGAGCTAGGCCGTCCACCGGCTGCTGGCGTCCTGGCCCCAGATA
TGTCCGACAAGGACAAGTGTTCAGCCATCTTCCGCTCGGACAGCTTGGGG
ACCCAGGGCCGGCTGAGCCGCACGCTGCCAGCCAGCGCGGAGGAGCGCGA
TCGGCTGCTGCGCCGCATGGAGAGCATGCGCAAGGAGAAGCGCGTGTACA
GCCGCTTCGAGGTCTTCTGCAAGAAAGAGGAGGCCAGCAGCCCTGGGGCA
GGGGAAGGCCCCGCGGAGGAGGGCACCAGGGACAGCAAGGTGGGCAAGTT
CGTGCCCAAGATCCTGGGCACGTTCAAAAGCAAGAAGtgagtcgac

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMARRSQSSSQGDNPLAPGY
LPPHYKEYYRLAVDALAEGGSEAYSRFLATEGAPDFLCPEELEHVSRHLR
PPQYVTREPPEGSLLDVDMDGSSGTYWPVNSDQAVPELDLGWPLTFGFQG
TEVTTLVQPPPPDSPSIKDEARRMIRSAQQVVAVVMDMFTDVDLLSEVLE
AAARRVPVYILLDEMNAQHFLDMADKCRVNLHHVDFLRVRTVAGPTYYCR
TGKSFKGHVKEKFLLVACAVVMSGSYSFMWSFEKIHRSLAHVFQGELVSS
FDEEFRILFAQSEPLVPSAAALARMDAYALAPYAGAGPLVGVPGVGAPTP
FSFPKRAHLLFPPPREEGLGFPSFLDPDRHFLSAFRREEPPRMPGGALEP
HAGLRPLSRRLEAEAGPAGELAGARGFFQARHLEMDAFKRHSFATEGAGA
VENFAAARQVSRQTFLSHGDDFRFQTSHFHRDQLYQQQYQWDPQLTPARP
QGLFEKLRGGRAGFADPDDFTLGAGPRFPELGPDGHQRLDYVPSSASREV
RHGSDPAFAPGPRGLEPSGAPRPNLTQRFPCQAAARPGPDPAPEAEPERR
GGPEGRAGLRRWRLASYLSGCHGEDGGDDGLPAPMEAEAYEDDVLAPGGR
APAGDLLPSAFRVPAAFPTKVPVPGPGSGGNGPEREGPEEPGLAKQDSFR
SRLNPLVQRSSRLRSSLIFSTSQAEGAAGAAAATEKVQLLHKEQTVSETL
GPGGEAVRSAASTKVAELLEKYKGPARDPGGGAGAITVASHSKAVVSQAW
REEVAAPGAVGGERRSLESCLLDLRDSFAQQLHQEAERQPGAASLTAAQL
LDTLGRSGSDRLPSRFLSAQSHSTSPQGLDSPLPLEGSGAHQVLHNESKG
SPTSAYPERKGSPTPGFSTRRGSPTTGFIEQKGSPTSAYPERRGSPVPPV
PERRSSPVPPVPERRGSLTLTISGESPKAGPAEEGPSGPMEVLRKGSLRL
RQLLSPKGERRMEDEGGFPVPQENGQPESPRRLSLGQGDSTEAATEERGP
RARLSSATANALYSSNLRDDTKAILEQISAHGQKHRAVPAPSPGPTHNSP
ELGRPPAAGVLAPDMSDKDKCSAIFRSDSLGTQGRLSRTLPASAEERDRL
LRRMESMRKEKRVYSRFEVFCKKEEASSPGAGEGPAEEGTRDSKVGKFVP
KILGTFKSKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28555
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent changes at Pro375 and Ala425 compared to NCBI ref.