cDNA Clone Mec1 (yeast)

• Plasmid: pGEX6P1 MEC1 (yeast) 1-222
• Parent Plasmid: pGEX6P1
• Expressed: GST MEC1 (yeast) 1-222
• Genbank: NP_009694.3
• Tag: MBP
• Species: Yeast

Sequence of Insert

GAATTCATGGAATCACACGTCAAATATCTTGACGAATTGATATTGGCAATAAAAGACCTGAACTCGGGGGTGGATTCAAAGGTGCAGATTAAAAAAGTGC
CCACGGATCCATCTTCTTCTCAGGAGTACGCCAAGAGTTTAAAGATCCTGAACACCCTCATAAGAAACCTAAAAGATCAAAGAAGGAACAATATCATGAA
AAATGATACTATATTTTCGAAAACAGTTTCCGCCCTTGCCTTATTGTTGGAGTACAACCCCTTCTTGCTTGTTATGAAGGATTCCAACGGGAACTTTGAG
ATACAAAGGCTGATAGATGATTTCCTCAACATATCCGTTCTGAACTATGATAATTACCACAGAATATGGTTTATGAGGCGAAAATTAGGCAGCTGGTGCA
AAGCATGTGTCGAATTTTACGGAAAACCTGCTAAGTTTCAGCTTACTGCACATTTTGAGAACACCATGAATCTTTACGAACAGGCCTTGACTGAAGTCTT
GTTGGGCAAGACTGAGCTTCTCAAATTTTATGACACCTTGAAGGGTCTATACATTCTTTTATACTGGTTCACTTCGGAGTATAGTACTTTTGGGAACTCT
ATAGCATTCTTAGATTCTTCTTTGGGGTTCACGAAATTTGACTTTAACTTCCAACGATTAATCAGGATTGTTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMESHVKYLDELILAIKDLNSGVDSKVQIKKVPTDPSSSQEYAKSLKILNTLIRNLKDQRRNNIMKN
DTIFSKTVSALALLLEYNPFLLVMKDSNGNFEIQRLIDDFLNISVLNYDNYHRIWFMRRKLGSWCKACVEFYGKPAKFQLTAHFENTMNLYEQALTEVLL
GKTELLKFYDTLKGLYILLYWFTSEYSTFGNSIAFLDSSLGFTKFDFNFQRLIRIV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU51943
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make constructs to express the N-terminus of S.cerevisiae Mec1 (1-222) in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in SHEEP.