cDNA Clone PODXL

• Plasmid: pcDNA5D FRT/TO HA PODXL (frameshift)
• Parent Plasmid: pcDNA5D FRT/TO HA
• Expressed: HA-PODXL (frameshift)
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCGCTGCGCGCTGGCGCTCTCGGCGCTGCTGTTACTGTTGTCAACGCCGCCGCTGCTGCCGTCGTCGCCGTCGCCGTCGCCGTCGCCGTCGCCCCCTCCCAGAATGCAACCCAGACTACTACGGACTCATCTAACAAAACAGCACCGACTCCAGCATCCAGTGTCACCATCATGGCTACAGATACAGCCCAGCAGAGCACAGTCCCCACTTCCAAGGCCAACGAAATCTTGGCCTCGGTCAAGGCGACCACCCTTGGTGTATCCAGTGACTCACCGGGGACTACAACCCTGGCTCAGCAAGTCTCAGGCCCAGTCAACACTACCGTGGCTAGAGGAGGCAGCTCAGGCAACCCTACTACCACCATCGAGAGCCCCAAGAGCACAAAAAGTGCAGACACCACTACAGTTGCAACCTCCACAGCCACAGCTAAACCTAACACCACAAGCAGCCAGAATGGAGCAGAAGATACAACAAACTCTGGGGGGAAAAGCAGCCACAGTGTGACCACAGACCTCACATCCACTAAGGCAGAACATCTGACGACCCCTCACCCTACAAGTCCACTTAGCCCCCGACAACCCACTTTGACGCATCCTGTGGCCACCCCAACAAGCTCGGGACATGACCATCTTATGAAAATTTCAAGCAGTTCAAGCACTGTGGCTATCCCTGGCTACACCTTCACAAGCCCGGGGATGACCACCACCCTACTAGAGACAGTGTTTCACCATGTCAGCCAGGCTGGTCTTGAACTCCTGACCTCGGGTGATCTGCCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGCGTCATCGGTTATCTCGCAAAGAACTCAACAGACCTCCAGTCAGATGCCAGCCAGCTCTACGGCCCCTTCCTCCCAGGAGACAGTGCAGCCCACGAGCCCGGCAACGGCATTGAGAACACCTACCCTGCCAGAGACCATGAGCTCCAGCCCCACAGCAGCATCAACTACCCACCGATACCCCAAAACACCTTCTCCCACTGTGGCTCATGAGAGTAACTGGGCAAAGTGTGAGGATCTTGAGACACAGACACAGAGTGAGAAGCAGCTCGTCCTGAACCTCACAGGAAACACCCTCTGTGCAGGGGGCGCTTCGGATGAGAAATTGATCTCACTGATATGCCGAGCAGTCAAAGCCACCTTCAACCCGGCCCAAGATAAGTGCGGCATACGGCTGGCATCTGTTCCAGGAAGTCAGACCGTGGTCGTCAAAGAAATCACTATTCACACTAAGCTCCCTGCCAAGGATGTGTACGAGCGGCTGAAGGACAAATGGGATGAACTAAAGGAGGCAGGGGTCAGTGACATGAAGCTAGGGGACCAGGGGCCACCGGAGGAGGCCGAGGACCGCTTCAGCATGCCCCTCATCATCACCATCGTCTGCATGGCATCATTCCTGCTCCTCGTGGCGGCCCTCTATGGCTGCTGCCACCAGCGCCTCTCCCAGAGGAAGGACCAGCAGCGGCTAACAGAGGAGCTGCAGACAGTGGAGAATGGTTACCATGACAACCCAACACTGGAAGTGATGGAGACCTCTTCTGAGATGCAGGAGAAGAAGGTGGTCAGCCTCAACGGGGAGCTGGGGGACAGCTGGATCGTCCCTCTGGACAACCTGACCAAGGACGACCTGGATGAGGAGGAAGACACACACCTCTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMRCALALSALLLLLSTPPLLPSSPSPSPSPSPPPRMQPRLLRTHLTKQHRLQHPVSPSWLQIQPSRAQSPLPRPTKSWPRSRRPPLVYPVTHRGLQPWLSKSQAQSTLPWLEEAAQATLLPPSRAPRAQKVQTPLQLQPPQPQLNLTPQAARMEQKIQQTLGGKAATVZPQTSHPLRQNIZRPLTLQVHLAPDNPLZRILWPPQQARDMTILZKFQAVQALWLSLATPSQARGZPPPYZRQCFTMSARLVLNSZPRVICPPWPPKVLGLQRHRLSRKELNRPPVRCQPALRPLPPRRQCSPRARQRHZEHLPCQRPZAPAPQQHQLPTDTPKHLLPLWLMRVTGQSVRILRHRHRVRSSSSZTSQETPSVQGALRMRNZSHZYAEQSKPPSTRPKISAAYGWHLFQEVRPWSSKKSLFTLSSLPRMCTSGZRTNGMNZRRQGSVTZSZGTRGHRRRPRTASACPSSSPSSAWHHSCSSWRPSMAAATSASPRGRTSSGZQRSCRQWRMVTMTTQHWKZWRPLLRCRRRRWSASTGSWGTAGSSLWTTZPRTTWMRRKTHTSRRP

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU26962
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: inserted frameshift mutation GTCGCCCC between nt89-90 ; Silent mutation L12 and 2 naturally occuring SNP's G112S S194L
• Low Temp: No