cDNA Clone alpha-Tubulin

• Synonyms: TUBA1A
• Plasmid: pBabeD puro GFP-TUBA1A
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP-TUBA1A
• Genbank: 7846
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGCGTGAGTGCATCTCCATCCACGTTGGCCAGGCTGGTGTCCA
GATTGGCAATGCCTGCTGGGAGCTCTACTGCCTGGAACACGGCATCCAGC
CCGATGGCCAGATGCCAAGTGACAAGACCATTGGGGGAGGAGATGACTCC
TTCAACACCTTCTTCAGTGAGACGGGCGCTGGCAAGCACGTGCCCCGGGC
TGTGTTTGTAGACTTGGAACCCACAGTCATTGATGAAGTTCGCACTGGCA
CCTACCGCCAGCTCTTCCACCCTGAGCAGCTCATCACAGGCAAGGAAGAT
GCTGCCAATAACTATGCCCGAGGGCACTACACCATTGGCAAGGAGATCAT
TGACCTTGTGTTGGACCGAATTCGCAAGCTGGCTGACCAGTGCACCGGTC
TTCAGGGCTTCTTGGTTTTCCACAGCTTTGGTGGGGGAACTGGTTCTGGG
TTCACCTCCCTGCTCATGGAACGTCTCTCAGTTGATTATGGCAAGAAATC
CAAGCTGGAGTTCTCCATTTACCCGGCACCCCAGGTTTCCACAGCTGTAG
TTGAGCCCTACAACTCCATCCTCACCACCCACACCACCCTGGAGCACTCT
GATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGACATCTGTCGTAG
AAACCTCGATATCGAGCGCCCAACCTACACTAACCTTAACCGCCTTATTG
GTCAGATTGTGTCCTCCATCACTGCTTCCCTGAGATTTGATGGAGCCCTG
AATGTTGACCTGACAGAATTCCAGACCAACCTGGTGCCCTACCCCCGCAT
CCACTTCCCTCTGGCCACATATGCCCCTGTCATCTCTGCTGAGAAAGCCT
ACCATGAACAGCTTTCTGTAGCAGAGATCACCAATGCTTGCTTTGAGCCA
GCCAACCAGATGGTGAAATGTGACCCTCGCCATGGTAAATACATGGCTTG
CTGCCTGTTGTACCGTGGTGACGTGGTTCCCAAAGATGTCAATGCTGCCA
TTGCCACCATCAAAACCAAGCGCACCATCCAGTTTGTGGATTGGTGCCCC
ACTGGCTTCAAGGTTGGCATCAACTACCAGCCTCCCACTGTGGTGCCTGG
TGGAGACCTGGCCAAGGTACAGAGAGCTGTGTGCATGCTGAGCAACACCA
CAGCCATTGCTGAGGCCTGGGCTCGCCTGGACCACAAGTTTGACCTGATG
TATGCCAAGCGTGCCTTTGTTCACTGGTACGTGGGTGAGGGGATGGAGGA
AGGCGAGTTTTCAGAGGCCCGTGAAGATATGGCTGCCCTTGAGAAGGATT
ATGAGGAGGTTGGTGTGGATTCTGTTGAAGGAGAGGGTGAGGAAGAAGGA
GAGGAATACTAAGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMRECI
SIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSE
TGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYAR
GHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLME
RLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVD
NEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEF
QTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKC
DPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGI
NYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFV
HWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28627
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 33932. Clone contains the following silent change compared to NCBI ref shown:; D47 (GAT > GAC), G57 (GGG > GGC), H61 (CAT > CAC), A65 (GCA > GCT), Q91 (CAA > CAG), L92 (CTT > CTC), K96 (AAA > AAG), L117 (CTC > CTT), A126 (GCC > GCT), T130 (ACG > ACC), L132 (CTC > CTT), S151 (TCG > TCC), K164 (AAG > AAA), S170 (TCT > TCC), A174 (GCG > GCA), I219 (ATT > ATC), R221 (CGT > CGC), Y224 (TAT > TAC), L227 (CTG > CTT), N228 (AAT > AAC), R229 (AGG > CGC), L230 (TTA > CTT), I231 (ATA > ATT), Q233 (CAA > CAG), Y262 (TAT > TAC), K336 (AAG > AAA), R339 (CGT > CGC), K401 (AAA > AAG), V409 (GTT > GTG), G410 (GGG > GGT), G416 (GGT > GGC), E423 (GAG > GAA), D424 (GAC > GAT),
• Low Temp: No