cDNA Clone EIF4G1

• Synonyms: eukaryotic translation initiation factor 4 gamma 1 tv7
• Plasmid: pcDNA5-FRT/TO-HA-EIF4G1 tv 2 R1205H
• Parent Plasmid: pcDNA5-FRT/TO-HA
• Expressed: HA-EIF4G1 tv 2 R1205H
• Genbank: NM_001194947.1 → NP_001181876.1
• Tag: HA
• Species: Human

Sequence of Insert

CTCGAGATGAACAAAGCTCCACAGTCCACAGGCCCCCCACCCGCCCCATC
CCCCGGACTCCCACAGCCAGCGTTTCCCCCGGGGCAGACAGCGCCGGTGG
TGTTCAGTACGCCACAAGCGACACAAATGAACACGCCTTCTCAGCCCCGC
CAGCACTTCTACCCTAGCCGGGCCCAGCCCCCGAGCAGTGCAGCCTCCCG
AGTGCAGAGTGCAGCCCCTGCCCGCCCTGGCCCAGCTGCCCATGTCTACC
CTGCTGGATCCCAAGTAATGATGATCCCTTCCCAGATCTCCTACCCAGCC
TCCCAGGGGGCCTACTACATCCCTGGACAGGGGCGTTCCACATACGTTGT
CCCGACACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAG
GTGCAAGCCCTACAGAATTTGGGACCTACGCTGGCGCCTACTATCCAGCC
CAAGGGGTGCAGCAGTTTCCCACTGGCGTGGCCCCCGCCCCAGTTTTGAT
GAACCAGCCACCCCAGATTGCTCCCAAGAGGGAGCGTAAGACGATCCGAA
TTCGAGATCCAAACCAAGGAGGAAAGGATATCACAGAGGAGATCATGTCT
GGGGCCCGCACTGCCTCCACACCCACCCCTCCCCAGACGGGAGGCGGTCT
GGAGCCTCAAGCTAATGGGGAGACGCCCCAGGTTGCTGTCATTGTCCGGC
CAGATGACCGGTCACAGGGAGCAATCATTGCTGACCGGCCAGGGCTGCCT
GGCCCAGAGCATAGCCCTTCAGAATCCCAGCCTTCGTCGCCTTCTCCGAC
CCCATCACCATCCCCAGTCTTGGAACCGGGGTCTGAGCCTAATCTCGCAG
TCCTCTCTATTCCTGGGGACACTATGACAACTATACAAATGTCTGTAGAA
GAATCAACCCCCATCTCCCGTGAAACTGGGGAGCCATATCGCCTCTCTCC
AGAACCCACTCCTCTCGCCGAACCCATACTGGAAGTAGAAGTGACACTTA
GCAAACCGGTTCCAGAATCTGAGTTTTCTTCCAGTCCTCTCCAGGCTCCC
ACCCCTTTGGCATCTCACACAGTGGAAATTCATGAGCCTAATGGCATGGT
CCCATCTGAAGATCTGGAACCAGAGGTGGAGTCAAGCCCAGAGCTTGCTC
CTCCCCCAGCTTGCCCCTCCGAATCCCCTGTGCCCATTGCTCCAACTGCC
CAACCTGAGGAACTGCTCAACGGAGCCCCCTCGCCACCAGCTGTGGACTT
AAGCCCAGTCAGTGAGCCAGAGGAGCAGGCCAAGGAGGTGACAGCATCAA
TGGCGCCCCCCACCATCCCCTCTGCTACTCCAGCTACGGCTCCTTCAGCT
ACTTCCCCAGCTCAGGAGGAGGAAATGGAAGAAGAAGAAGAAGAGGAAGA
AGGAGAAGCAGGAGAAGCAGGAGAAGCTGAGAGTGAGAAAGGAGGAGAGG
AACTGCTCCCCCCAGAGAGTACCCCTATTCCAGCCAACTTGTCTCAGAAT
TTGGAGGCAGCAGCAGCCACTCAAGTGGCAGTATCTGTGCCAAAGAGGAG
ACGGAAAATTAAGGAGCTAAATAAGAAGGAGGCTGTTGGAGACCTTCTGG
ATGCCTTCAAGGAGGCGAACCCGGCAGTACCAGAGGTGGAAAATCAGCCT
CCTGCAGGCAGCAATCCAGGCCCAGAGTCTGAGGGCAGTGGTGTGCCCCC
ACGTCCTGAGGAAGCAGATGAGACCTGGGACTCAAAGGAAGACAAAATTC
ACAATGCTGAGAACATCCAGCCCGGGGAACAGAAGTATGAATATAAGTCA
GATCAGTGGAAGCCTCTAAACCTAGAGGAGAAAAAACGTTACGACCGTGA
GTTCCTGCTTGGTTTTCAGTTCATCTTTGCCAGTATGCAGAAGCCAGAGG
GATTGCCACATATCAGTGACGTGGTGCTGGACAAGGCCAATAAAACACCA
CTGCGGCCACTGGATCCCACTAGACTACAAGGCATAAATTGTGGCCCAGA
CTTCACTCCATCCTTTGCCAACCTTGGCCGGACAACCCTTAGCACCCGTG
GGCCCCCAAGGGGTGGGCCAGGTGGGGAGCTGCCCCGTGGGCCGGCTGGC
CTGGGACCCCGGCGCTCTCAGCAGGGACCCCGAAAAGAACCACGCAAGAT
CATTGCCACAGTGTTAATGACCGAAGATATAAAACTGAACAAAGCAGAGA
AAGCCTGGAAACCCAGCAGCAAGCGGACGGCGGCTGATAAGGATCGAGGG
GAAGAAGATGCTGATGGCAGCAAAACCCAGGACCTATTCCGCAGGGTGCG
CTCCATCCTGAATAAACTGACACCCCAGATGTTCCAGCAGCTGATGAAGC
AAGTGACGCAGCTGGCCATCGACACCGAGGAACGCCTCAAAGGGGTCATT
GACCTCATTTTTGAGAAGGCCATTTCAGAGCCCAACTTCTCTGTGGCCTA
TGCCAACATGTGCCGCTGCCTCATGGCGCTGAAAGTGCCCACTACGGAAA
AGCCAACAGTGACTGTGAACTTCCGAAAGCTGTTGTTGAATCGATGTCAG
AAGGAGTTTGAGAAAGACAAAGATGATGATGAGGTTTTTGAGAAGAAGCA
AAAAGAGATGGATGAAGCTGCTACGGCAGAGGAACGAGGACGCCTGAAGG
AAGAGCTGGAAGAGGCTCGGGACATAGCCCGGCGGCGCTCTTTAGGGAAT
ATCAAGTTTATTGGAGAGTTGTTCAAACTGAAGATGTTAACAGAGGCAAT
AATGCATGACTGTGTGGTCAAACTGCTTAAGAACCATGATGAAGAGTCCC
TTGAGTGCCTTTGTCGTCTGCTCACCACCATTGGCAAAGACCTGGACTTT
GAAAAAGCCAAGCCCCGAATGGATCAGTATTTCAACCAGATGGAAAAAAT
CATTAAAGAAAAGAAGACGTCATCCCGCATCCGCTTTATGCTGCAGGACG
TGCTGGATCTGCGAGGGAGCAATTGGGTGCCACGCCGAGGGGATCAGGGT
CCCAAGACCATTGACCAGATCCATAAGGAGGCTGAGATGGAAGAACATCG
AGAGCACATCAAAGTGCAGCAGCTCATGGCCAAGGGCAGTGACAAGCGTC
GGGGCGGTCCTCCAGGCCCTCCCATCAGCCGTGGACTTCCCCTTGTGGAT
GATGGTGGCTGGAACACAGTTCCCATCAGCAAAGGTAGCCGCCCCATTGA
CACCTCACGACTCACCAAGATCACCAAGCCTGGCTCCATCGATTCTAACA
ACCAGCTCTTTGCACCTGGAGGGCGACTGAGCTGGGGCAAGGGCAGCAGC
GGAGGCTCAGGAGCCAAGCCCTCAGACGCAGCATCAGAAGCTGCTCGCCC
AGCTACTAGTACTTTGAATCGCTTCTCAGCCCTTCAACAAGCGGTACCCA
CAGAAAGCACAGATAATAGACGTGTGGTGCAGAGGAGTAGCTTGAGCCGA
GAACGAGGCGAGAAAGCTGGAGACCGAGGAGACCGCCTAGAGCGGAGTGA
ACGGGGAGGGGACCGTGGGGACCGGCTTGATCGTGCGCGGACACCTGCTA
CCAAGCGGAGCTTCAGCAAGGAAGTGGAGGAGCGGAGTAGAGAACGGCCC
TCCCAGCCTGAGGGGCTGCaCAAGGCAGCTAGCCTCACGGAGGATCGGGA
CCGTGGGCGGGATGCCGTGAAGCGAGAAGCTGCCCTACCCCCAGTGAGCC
CCCTGAAGGCGGCTCTCTCTGAGGAGGAGTTAGAGAAGAAATCCAAGGCT
ATCATTGAGGAATATCTCCATCTCAATGACATGAAAGAGGCAGTCCAGTG
CGTGCAGGAGCTGGCCTCACCCTCCTTGCTCTTCATCTTTGTACGGCATG
GTGTCGAGTCTACGCTGGAGCGCAGTGCCATTGCTCGTGAGCATATGGGG
CAGCTGCTGCACCAGCTGCTCTGTGCTGGGCATCTGTCTACTGCTCAGTA
CTACCAAGGGTTGTATGAAATCTTGGAATTGGCTGAGGACATGGAAATTG
ACATCCCCCACGTGTGGCTCTACCTAGCGGAACTGGTAACACCCATTCTG
CAGGAAGGTGGGGTGCCCATGGGGGAGCTGTTCAGGGAGATTACAAAGCC
TCTGAGACCGTTGGGCAAAGCTGCTTCCCTGTTGCTGGAGATCCTGGGCC
TCCTGTGCAAAAGCATGGGTCCTAAAAAGGTGGGGACGCTGTGGCGAGAA
GCCGGGCTTAGCTGGAAGGAATTTCTACCTGAAGGCCAGGACATTGGTGC
ATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCGGAAG
CCCCTGGCCAGAGGGCACTCCCCTCCGAGGAGCTGAACAGGCAGCTGGAG
AAGCTGCTGAAGGAGGGCAGCAGTAACCAGCGGGTGTTCGACTGGATAGA
GGCCAACCTGAGTGAGCAGCAGATAGTATCCAACACGTTAGTTCGAGCCC
TCATGACGGCTGTCTGCTATTCTGCAATTATTTTTGAGACTCCCCTCCGA
GTGGACGTTGCAGTGCTGAAAGCGCGAGCGAAGCTGCTGCAGAAATACCT
GTGTGACGAGCAGAAGGAGCTACAGGCGCTCTACGCCCTCCAGGCCCTTG
TAGTGACCTTAGAACAGCCTCCCAACCTGCTGCGGATGTTCTTTGACGCA
CTGTATGACGAGGACGTGGTGAAGGAGGATGCCTTCTACAGTTGGGAGAG
TAGCAAGGACCCCGCTGAGCAGCAGGGCAAGGGTGTGGCCCTTAAATCTG
TCACAGCCTTCTTCAAGTGGCTCCGTGAAGCAGAGGAGGAGTCTGACCAC
AACTGAgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSPGEFASLEMNKAPQSTGPPPAPSPGLPQPAFPPGQTAP
VVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHV
YPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFY
PGASPTEFGTYAGAYYPAQGVQQFPTGVAPAPVLMNQPPQIAPKRERKTI
RIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIV
RPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNL
AVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVT
LSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPEL
APPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTA
SMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGG
EELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDL
LDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDK
IHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKP
EGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLST
RGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKA
EKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLM
KQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTT
EKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRL
KEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEE
SLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQ
DVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDK
RRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDS
NNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAV
PTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTP
ATKRSFSKEVEERSRERPSQPEGLHKAASLTEDRDRGRDAVKREAALPPV
SPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVR
HGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDME
IDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEIL
GLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEES
EAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVR
ALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQA
LVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALK
SVTAFFKWLREAEEESDHN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28631
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41456. tv2 (isoform 5). created by deletion of 7 residue insertion at position 45 in tv7. in isoform 5 and tv7 Q697 is deleted (compared with isoform 1). clone contains Thr161Ala SNP Grow at or below 30ºC Grow at or below 30ºC
• Low Temp: Yes