cDNA Clone FAM83G FAM83H

• Plasmid: pSc-B-FAM83G (M1-V310)-FAM83H (V285-K1179)
• Parent Plasmid: pSC-B
• Species: Human

Sequence of Insert

ggatccATGGCCTTCTCTCAGGTGCAGTGTCTGGACGACAACCATGTGAA
CTGGCGCTCCAGCGAGTCCAAGCCTGAGTTCTTCTACAGCGAGGAGCAGC
GGCTGGCGCTGGAGGCCCTGGTGGCCCGCGGCCGGGACGCCTTCTACGAG
GTGCTCAAGCGGGAGAACATCCGAGACTTCCTCTCGGAGCTGGAGCTCAA
GCGCATCCTGGAGACCATCGAGGTGTACGACCCGGGCTCTGAGGACCCTC
GGGGCACGGGCCCCTCTCAGGGGCCCGAGGACAATGGGGTCGGCGACGGC
GAGGAAGCCAGCGGGGCGGATGGGGTCCCCATCGAGGCCGAGCCGCTGCC
CTCCCTGGAGTACTGGCCCCAGAAGTCGGACCGCTCCATCCCGCAGCTGG
ACCTGGGCTGGCCCGACACCATCGCCTACCGCGGCGTGACCCGGGCTAGC
GTCTACATGCAGCCCCCCATAGACGGGCAGGCCCACATCAAAGAGGTGGT
GCGGAAGATGATCAGCCAGGCACAGAAGGTGATAGCTGTGGTCATGGACA
TGTTCACCGACGTGGACATCTTCAAGGACCTGCTGGACGCCGGCTTCAAG
AGGAAAGTGGCCGTGTACATCATCGTGGATGAGAGTAACGTCAAGTACTT
CCTGCACATGTGTGAGCGGGCCTGCATGCACCTGGGGCACCTCAAGAATC
TCAGAGTGCGGAGCAGCGGGGGAACTGAGTTCTTCACGCGGTCGGCAACC
AAGTTCAAGGGTGCCCTGGCCCAGAAGTTCATGTTTGTGGATGGAGACCG
GGCTGTGTGCGGCTCCTACAGCTTCACGTGGTCGGCCGCGCGGACGGACC
GGAATGTGATCTCTGTGCTGTCTGGCCAGGTGGTGGAGATGTTTGACCGG
CAGTTCCAGGAGCTGTACCTCATGTCACACAGTGTGGTGCCCTCGGCCGC
GGCCCTGGCCCGCATGGACGCCTATGCCCTGGCTCCGTATGCCGGGGCCG
GGCCTCTCGTGGGCGTCCCTGGGGTCGGGGCGCCAACCCCCTTCTCCTTC
CCTAAACGAGCGCACCTCCTGTTCCCGCCACCCCGGGAAGAGGGCCTGGG
CTTCCCCTCCTTCCTCGACCCGGACCGCCACTTCCTGTCGGCCTTCCGCC
GGGAGGAGCCGCCGCGGATGCCGGGGGGCGCGCTGGAACCGCACGCGGGG
CTGCGGCCACTCTCGCGGCGCCTGGAGGCCGAGGCCGGGCCGGCTGGGGA
GCTCGCGGGCGCGCGGGGCTTCTTCCAGGCGCGGCACCTGGAGATGGACG
CCTTCAAGCGGCACAGCTTCGCGACCGAGGGCGCGGGCGCCGTGGAGAAC
TTCGCGGCTGCGCGGCAGGTGTCGCGGCAGACGTTCCTCAGCCACGGCGA
CGACTTCCGCTTCCAGACCAGCCACTTCCACCGTGACCAGCTCTACCAGC
AGCAGTACCAGTGGGACCCGCAGCTCACGCCGGCGCGCCCGCAAGGCCTG
TTCGAGAAGCTTCGCGGGGGCCGCGCGGGTTTCGCGGACCCGGATGACTT
CACCCTGGGCGCCGGGCCCCGCTTCCCGGAGCTCGGACCCGACGGGCACC
AGCGGCTGGACTACGTGCCGTCCAGCGCGTCCCGCGAGGTGCGCCACGGC
TCGGACCCCGCCTTCGCGCCCGGACCCCGCGGCCTGGAGCCCAGCGGAGC
CCCGCGCCCCAACCTGACCCAGCGCTTCCCATGCCAGGCCGCGGCGAGGC
CGGGCCCAGACCCCGCTCCCGAGGCGGAGCCGGAGCGCAGGGGCGGGCCC
GAGGGGCGGGCAGGGCTGCGGCGCTGGCGTTTGGCCTCCTACTTGAGCGG
CTGCCACGGCGAGGATGGGGGCGACGACGGCCTACCGGCGCCCATGGAAG
CGGAGGCTTACGAAGACGACGTGCTGGCTCCCGGGGGCCGGGCACCTGCC
GGCGACCTGCTCCCCTCGGCCTTCCGCGTCCCAGCAGCCTTCCCCACCAA
GGTCCCGGTGCCAGGCCCGGGCAGCGGCGGCAACGGCCCAGAGCGCGAGG
GCCCGGAGGAGCCTGGCCTGGCCAAGCAGGACTCATTCCGCTCGCGCCTG
AACCCCCTGGTCCAGCGCAGCTCCAGGCTGCGCTCCTCGCTCATCTTCAG
CACGTCACAGGCCGAGGGCGCGGCCGGGGCTGCGGCGGCCACTGAGAAGG
TGCAGCTGCTGCACAAGGAGCAGACGGTCAGCGAGACGCTGGGGCCCGGC
GGAGAGGCCGTGCGCTCCGCGGCTTCCACCAAGGTGGCGGAGCTGCTGGA
GAAGTACAAGGGCCCAGCCCGTGATCCCGGCGGCGGCGCGGGCGCCATCA
CCGTTGCCAGCCACAGCAAGGCCGTCGTGTCCCAGGCGTGGCGGGAAGAG
GTGGCGGCCCCAGGTGCCGTGGGGGGCGAGCGCCGCAGCCTCGAGAGCTG
CCTGCTGGACCTGCGCGACTCCTTTGCACAGCAGCTGCACCAGGAGGCGG
AGCGGCAGCCGGGAGCCGCGTCGCTCACCGCGGCGCAGCTGCTCGACACA
CTGGGCCGGAGCGGCTCCGACCGCCTGCCTTCCCGCTTCCTCTCTGCCCA
GAGCCACTCAACGTCCCCGCAAGGGCTGGACAGCCCTCTGCCGCTGGAAG
GGTCCGGAGCGCACCAGGTGCTCCATAATGAGTCAAAAGGGAGCCCCACC
TCGGCTTACCCTGAGCGGAAGGGGAGCCCCACGCCTGGGTTTTCCACTCG
AAGAGGAAGTCCAACTACAGGATTTATCGAGCAGAAGGGGAGCCCCACCT
CAGCCTACCCCGAGCGCAGGGGTAGTCCGGTGCCCCCCGTGCCGGAGCGC
AGGAGCAGTCCGGTGCCCCCCGTGCCGGAGCGCAGGGGCAGCCTCACCCT
TACCATCTCCGGGGAGTCCCCGAAGGCCGGGCCCGCGGAGGAGGGGCCGA
GCGGCCCCATGGAAGTCCTGCGCAAAGGCTCCTTGCGTCTTAGGCAGCTG
CTGAGCCCCAAGGGCGAGCGGCGCATGGAGGATGAGGGTGGCTTCCCAGT
GCCGCAGGAGAACGGCCAACCCGAGAGCCCGCGGCGTCTGTCACTGGGCC
AGGGTGACAGCACGGAGGCTGCCACAGAAGAGCGGGGTCCGCGGGCGCGC
CTGTCCTCAGCCACGGCCAACGCCTTGTACAGCAGCAACCTTCGGGATGA
CACGAAGGCCATTCTGGAGCAGATCAGTGCCCACGGCCAGAAGCACCGTG
CGGTCCCTGCCCCGAGCCCCGGCCCGACCCACAACAGCCCCGAGCTAGGC
CGTCCACCGGCTGCTGGCGTCCTGGCCCCAGATATGTCCGACAAGGACAA
GTGTTCAGCCATCTTCCGCTCGGACAGCTTGGGGACCCAGGGCCGGCTGA
GCCGCACGCTGCCAGCCAGCGCGGAGGAGCGCGATCGGCTGCTGCGCCGC
ATGGAGAGCATGCGCAAGGAGAAGCGCGTGTACAGCCGCTTCGAGGTCTT
CTGCAAGAAAGAGGAGGCCAGCAGCCCTGGGGCAGGGGAAGGCCCCGCGG
AGGAGGGCACCAGGGACAGCAAGGTGGGCAAGTTCGTGCCCAAGATCCTG
GGCACGTTCAAAAGCAAGAAGTGAgcggccgc

Amino Acid Sequence

MAFSQVQCLDDNHVNWRSSESKPEFFYSEEQRLALEALVARGRDAFYEVL
KRENIRDFLSELELKRILETIEVYDPGSEDPRGTGPSQGPEDNGVGDGEE
ASGADGVPIEAEPLPSLEYWPQKSDRSIPQLDLGWPDTIAYRGVTRASVY
MQPPIDGQAHIKEVVRKMISQAQKVIAVVMDMFTDVDIFKDLLDAGFKRK
VAVYIIVDESNVKYFLHMCERACMHLGHLKNLRVRSSGGTEFFTRSATKF
KGALAQKFMFVDGDRAVCGSYSFTWSAARTDRNVISVLSGQVVEMFDRQF
QELYLMSHSVVPSAAALARMDAYALAPYAGAGPLVGVPGVGAPTPFSFPK
RAHLLFPPPREEGLGFPSFLDPDRHFLSAFRREEPPRMPGGALEPHAGLR
PLSRRLEAEAGPAGELAGARGFFQARHLEMDAFKRHSFATEGAGAVENFA
AARQVSRQTFLSHGDDFRFQTSHFHRDQLYQQQYQWDPQLTPARPQGLFE
KLRGGRAGFADPDDFTLGAGPRFPELGPDGHQRLDYVPSSASREVRHGSD
PAFAPGPRGLEPSGAPRPNLTQRFPCQAAARPGPDPAPEAEPERRGGPEG
RAGLRRWRLASYLSGCHGEDGGDDGLPAPMEAEAYEDDVLAPGGRAPAGD
LLPSAFRVPAAFPTKVPVPGPGSGGNGPEREGPEEPGLAKQDSFRSRLNP
LVQRSSRLRSSLIFSTSQAEGAAGAAAATEKVQLLHKEQTVSETLGPGGE
AVRSAASTKVAELLEKYKGPARDPGGGAGAITVASHSKAVVSQAWREEVA
APGAVGGERRSLESCLLDLRDSFAQQLHQEAERQPGAASLTAAQLLDTLG
RSGSDRLPSRFLSAQSHSTSPQGLDSPLPLEGSGAHQVLHNESKGSPTSA
YPERKGSPTPGFSTRRGSPTTGFIEQKGSPTSAYPERRGSPVPPVPERRS
SPVPPVPERRGSLTLTISGESPKAGPAEEGPSGPMEVLRKGSLRLRQLLS
PKGERRMEDEGGFPVPQENGQPESPRRLSLGQGDSTEAATEERGPRARLS
SATANALYSSNLRDDTKAILEQISAHGQKHRAVPAPSPGPTHNSPELGRP
PAAGVLAPDMSDKDKCSAIFRSDSLGTQGRLSRTLPASAEERDRLLRRME
SMRKEKRVYSRFEVFCKKEEASSPGAGEGPAEEGTRDSKVGKFVPKILGT
FKSKK*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU28636
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: FAM83G DUF domain M1-V310 amplified from 24465 with NW2881/NW4547.; FAM83H non DUF domain V285-K179 amplified with NW4548/49; Used the two products in an overlap reaction with NW2881/4549 to generate chimera flanked Bam-Not
• Low Temp: No