cDNA Clone ASC

• Synonyms: PYCARD, CARD5, TMS, TMS-1, TMS1
• Plasmid: pBABED P FLAG VHL a-ASC nanobody
• Parent Plasmid: pBABED P FLAG
• Expressed: FLAG-VHL-5xGLY-aASC nanobody
• Species: Human

Sequence of Insert

VHL
GGATCCGCCATGGACTACAAGGACGATGACGATAAGGGATCCGCCATGCCCCGGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCAGGCGTCGAAGAGTACGGCCCTGAAGAAGACGGCGGGGAGGAGTCGGGCGCCGAGGAGTCCGGCCCGGAAGAGTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGAGATGGAGGCCGGTCGGCCGCGGCCCGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGTCCGCGCGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACGCTGCCGCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAGAGATGCAGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTCAATGTTGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAGCGATGCCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATCGTCAGGTCGCTCTACGAAGATCTGGAAGACCACCCAAATGTGCAGAAAGACCTGGAGCGGCTGACACAGGAGCGCATTGCACATCAACGGATGGGAGATGGTGGAGGCGGAGGT

aASC
GAATTCGCCATGGCTCAGGTCCAGCTGCAAGAGTCTGGCGGAGGACTTGTTCAGCCTGGCGGAAGCCTGAAGCTGTCTTGTGCCGCCAGCGGCTTCACCTTCAGCAGATACGCCATGAGCTGGTACAGACAGGCCCCTGGCAAAGAAAGGGAAAGCGTGGCCAGAATCAGCAGCGGCGGAGGCACAATCTACTACGCCGATAGCGTGAAGGGCAGATTCACCATCTCCAGAGAGGACGCCAAGAACACCGTGTACCTGCAGATGAACTCCCTGAAGCCTGAGGACACCGCCGTGTACTACTGTTACGTCGGCGGCTTTTGGGGCCAGGGCACACAAGTGACAGTGTCTAGCGGAGGCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSAMPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVW
LNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDL
EDHPNVQKDLERLTQERIAHQRMGDGGGGGEFAMAQVQLQESGGGLVQPGGSLKLSCAASGFTFSRYAMSWYRQAPGKERESVARISSGGGTIYYADSVK
GRFTISREDAKNTVYLQMNSLKPEDTAVYYCYVGGFWGQGTQVTVSSGG*

• Vector Type: Other
• Antibiotic: Amp
• Full Snapgene Map: pBABED P FLAG VHL a-ASC nanobody78.96 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU54832
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Anti-ASC nanobody for use in degron work. Codon optimised.
• Low Temp: No