cDNA Clone FAM83H

• Plasmid: pCS2+ GFP FAM83H F274A
• Parent Plasmid: pCS2+ GFP
• Expressed: GFP FAM83H F274A
• Genbank: NM_198488.3
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCCCGTCGCTCTCAGAGCTCCTCGCAGGGGGACAACCCACTGGCACCCGGGTACCTGCCGCCTCACTACAAAGAGTACTACCGCCTGGCGGTGGATGCACTGGCCGAGGGTGGCTCGGAGGCCTACAGCCGCTTCCTCGCTACCGAGGGGGCACCAGACTTCCTGTGCCCTGAAGAGCTGGAACATGTGAGCCGACACCTTCGGCCTCCGCAGTATGTTACCCGAGAGCCACCTGAAGGCAGCCTTCTCGACGTGGACATGGATGGCTCCTCGGGTACATACTGGCCAGTGAACTCAGACCAGGCCGTGCCTGAGCTTGATTTGGGCTGGCCTCTGACCTTCGGCTTCCAGGGCACCGAGGTGACCACCTTGGTGCAGCCACCGCCCCCCGACAGCCCCAGTATCAAGGATGAGGCCCGCAGGATGATCCGTTCCGCCCAGCAGGTGGTGGCCGTGGTGATGGACATGTTCACTGATGTGGACCTGCTCAGCGAAGTGCTGGAGGCCGCGGCCCGTCGGGTCCCAGTCTACATCCTGCTGGATGAGATGAACGCGCAGCACTTCCTGGACATGGCCGACAAGTGCCGTGTCAACCTGCACCACGTGGATTTCCTGCGCGTACGGACTGTGGCGGGCCCCACCTACTACTGCCGCACTGGGAAGTCCTTCAAGGGCCACGTCAAGGAGAAGTTCCTGCTGGTGGACTGTGCCGTGGTGATGAGTGGGAGCTACAGCTTCATGTGGTCCTTTGAGAAGATCCACCGCAGCCTGGCGCACGTGTTCCAAGGAGAGCTGGTCTCCAGCTTCGACGAGGAGGCCCGCATCCTCTTCGCGCAGTCCGAGCCGCTTGTGCCCTCGGCCGCGGCCCTGGCCCGCATGGACGCCTATGCCCTGGCTCCGTATGCCGGGGCCGGGCCTCTCGTGGGCGTCCCTGGGGTCGGGGCGCCAACCCCCTTCTCCTTCCCTAAACGAGCGCACCTCCTGTTCCCGCCACCCCGGGAAGAGGGCCTGGGCTTCCCCTCCTTCCTCGACCCGGACCGCCACTTCCTGTCGGCCTTCCGCCGGGAGGAGCCGCCGCGGATGCCGGGGGGCGCGCTGGAACCGCACGCGGGGCTGCGGCCACTCTCGCGGCGCCTGGAGGCCGAGGCCGGGCCGGCTGGGGAGCTCGCGGGCGCGCGGGGCTTCTTCCAGGCGCGGCACCTGGAGATGGACGCCTTCAAGCGGCACAGCTTCGCGACCGAGGGCGCGGGCGCCGTGGAGAACTTCGCGGCTGCGCGGCAGGTGTCGCGGCAGACGTTCCTCAGCCACGGCGACGACTTCCGCTTCCAGACCAGCCACTTCCACCGTGACCAGCTCTACCAGCAGCAGTACCAGTGGGACCCGCAGCTCACGCCGGCGCGCCCGCAAGGCCTGTTCGAGAAGCTTCGCGGGGGCCGCGCGGGTTTCGCGGACCCGGATGACTTCACCCTGGGCGCCGGGCCCCGCTTCCCGGAGCTCGGACCCGACGGGCACCAGCGGCTGGACTACGTGCCGTCCAGCGCGTCCCGCGAGGTGCGCCACGGCTCGGACCCCGCCTTCGCGCCCGGACCCCGCGGCCTGGAGCCCAGCGGAGCCCCGCGCCCCAACCTGACCCAGCGCTTCCCATGCCAGGCCGCGGCGAGGCCGGGCCCAGACCCCGCTCCCGAGGCGGAGCCGGAGCGCAGGGGCGGGCCCGAGGGGCGGGCAGGGCTGCGGCGCTGGCGTTTGGCCTCCTACTTGAGCGGCTGCCACGGCGAGGATGGGGGCGACGACGGCCTACCGGCGCCCATGGAAGCGGAGGCTTACGAAGACGACGTGCTGGCTCCCGGGGGCCGGGCACCTGCCGGCGACCTGCTCCCCTCGGCCTTCCGCGTCCCAGCAGCCTTCCCCACCAAGGTCCCGGTGCCAGGCCCGGGCAGCGGCGGCAACGGCCCAGAGCGCGAGGGCCCGGAGGAGCCTGGCCTGGCCAAGCAGGACTCATTCCGCTCGCGCCTGAACCCCCTGGTCCAGCGCAGCTCCAGGCTGCGCTCCTCGCTCATCTTCAGCACGTCACAGGCCGAGGGCGCGGCCGGGGCTGCGGCGGCCACTGAGAAGGTGCAGCTGCTGCACAAGGAGCAGACGGTCAGCGAGACGCTGGGGCCCGGCGGAGAGGCCGTGCGCTCCGCGGCTTCCACCAAGGTGGCGGAGCTGCTGGAGAAGTACAAGGGCCCAGCCCGTGATCCCGGCGGCGGCGCGGGCGCCATCACCGTTGCCAGCCACAGCAAGGCCGTCGTGTCCCAGGCGTGGCGGGAAGAGGTGGCGGCCCCAGGTGCCGTGGGGGGCGAGCGCCGCAGCCTCGAGAGCTGCCTGCTGGACCTGCGCGACTCCTTTGCACAGCAGCTGCACCAGGAGGCGGAGCGGCAGCCGGGAGCCGCGTCGCTCACCGCGGCGCAGCTGCTCGACACACTGGGCCGGAGCGGCTCCGACCGCCTGCCTTCCCGCTTCCTCTCTGCCCAGAGCCACTCAACGTCCCCGCAAGGGCTGGACAGCCCTCTGCCGCTGGAAGGGTCCGGAGCGCACCAGGTGCTCCATAATGAGTCAAAAGGGAGCCCCACCTCGGCTTACCCTGAGCGGAAGGGGAGCCCCACGCCTGGGTTTTCCACTCGAAGAGGAAGTCCAACTACAGGATTTATCGAGCAGAAGGGGAGCCCCACCTCAGCCTACCCCGAGCGCAGGGGTAGTCCGGTGCCCCCCGTGCCGGAGCGCAGGAGCAGTCCGGTGCCCCCCGTGCCGGAGCGCAGGGGCAGCCTCACCCTTACCATCTCCGGGGAGTCCCCGAAGGCCGGGCCCGCGGAGGAGGGGCCGAGCGGCCCCATGGAAGTCCTGCGCAAAGGCTCCTTGCGTCTTAGGCAGCTGCTGAGCCCCAAGGGCGAGCGGCGCATGGAGGATGAGGGTGGCTTCCCAGTGCCGCAGGAGAACGGCCAACCCGAGAGCCCGCGGCGTCTGTCACTGGGCCAGGGTGACAGCACGGAGGCTGCCACAGAAGAGCGGGGTCCGCGGGCGCGCCTGTCCTCAGCCACGGCCAACGCCTTGTACAGCAGCAACCTTCGGGATGACACGAAGGCCATTCTGGAGCAGATCAGTGCCCACGGCCAGAAGCACCGTGCGGTCCCTGCCCCGAGCCCCGGCCCGACCCACAACAGCCCCGAGCTAGGCCGTCCACCGGCTGCTGGCGTCCTGGCCCCAGATATGTCCGACAAGGACAAGTGTTCAGCCATCTTCCGCTCGGACAGCTTGGGGACCCAGGGCCGGCTGAGCCGCACGCTGCCAGCCAGCGCGGAGGAGCGCGATCGGCTGCTGCGCCGCATGGAGAGCATGCGCAAGGAGAAGCGCGTGTACAGCCGCTTCGAGGTCTTCTGCAAGAAAGAGGAGGCCAGCAGCCCTGGGGCAGGGGAAGGCCCCGCGGAGGAGGGCACCAGGGACAGCAAGGTGGGCAAGTTCGTGCCCAAGATCCTGGGCACGTTCAAAAGCAAGAAGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSMARRSQSSS
QGDNPLAPGYLPPHYKEYYRLAVDALAEGGSEAYSRFLATEGAPDFLCPE
ELEHVSRHLRPPQYVTREPPEGSLLDVDMDGSSGTYWPVNSDQAVPELDL
GWPLTFGFQGTEVTTLVQPPPPDSPSIKDEARRMIRSAQQVVAVVMDMFT
DVDLLSEVLEAAARRVPVYILLDEMNAQHFLDMADKCRVNLHHVDFLRVR
TVAGPTYYCRTGKSFKGHVKEKFLLVDCAVVMSGSYSFMWSFEKIHRSLA
HVFQGELVSSFDEEARILFAQSEPLVPSAAALARMDAYALAPYAGAGPLV
GVPGVGAPTPFSFPKRAHLLFPPPREEGLGFPSFLDPDRHFLSAFRREEP
PRMPGGALEPHAGLRPLSRRLEAEAGPAGELAGARGFFQARHLEMDAFKR
HSFATEGAGAVENFAAARQVSRQTFLSHGDDFRFQTSHFHRDQLYQQQYQ
WDPQLTPARPQGLFEKLRGGRAGFADPDDFTLGAGPRFPELGPDGHQRLD
YVPSSASREVRHGSDPAFAPGPRGLEPSGAPRPNLTQRFPCQAAARPGPD
PAPEAEPERRGGPEGRAGLRRWRLASYLSGCHGEDGGDDGLPAPMEAEAY
EDDVLAPGGRAPAGDLLPSAFRVPAAFPTKVPVPGPGSGGNGPEREGPEE
PGLAKQDSFRSRLNPLVQRSSRLRSSLIFSTSQAEGAAGAAAATEKVQLL
HKEQTVSETLGPGGEAVRSAASTKVAELLEKYKGPARDPGGGAGAITVAS
HSKAVVSQAWREEVAAPGAVGGERRSLESCLLDLRDSFAQQLHQEAERQP
GAASLTAAQLLDTLGRSGSDRLPSRFLSAQSHSTSPQGLDSPLPLEGSGA
HQVLHNESKGSPTSAYPERKGSPTPGFSTRRGSPTTGFIEQKGSPTSAYP
ERRGSPVPPVPERRSSPVPPVPERRGSLTLTISGESPKAGPAEEGPSGPM
EVLRKGSLRLRQLLSPKGERRMEDEGGFPVPQENGQPESPRRLSLGQGDS
TEAATEERGPRARLSSATANALYSSNLRDDTKAILEQISAHGQKHRAVPA
PSPGPTHNSPELGRPPAAGVLAPDMSDKDKCSAIFRSDSLGTQGRLSRTL
PASAEERDRLLRRMESMRKEKRVYSRFEVFCKKEEASSPGAGEGPAEEGT
RDSKVGKFVPKILGTFKSKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28659
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent changes at Pro375 and Ala425 compared to NCBI ref. Parental 28161. F270 restored
• Low Temp: No