cDNA Clone FAM83H

• Plasmid: pBabeD puro FAM83H F274A
• Parent Plasmid: pBabeD puro
• Expressed: FAM83H F274A
• Genbank: NM_198488.3
• Species: Human

Sequence of Insert

GGATCCATGGCCCGTCGCTCTCAGAGCTCCTCGCAGGGGGACAACCCACTGGCACCCGGGTACCTGCCGCCTCACTACAAAGAGTACTACCGCCTGGCGGTGGATGCACTGGCCGAGGGTGGCTCGGAGGCCTACAGCCGCTTCCTCGCTACCGAGGGGGCACCAGACTTCCTGTGCCCTGAAGAGCTGGAACATGTGAGCCGACACCTTCGGCCTCCGCAGTATGTTACCCGAGAGCCACCTGAAGGCAGCCTTCTCGACGTGGACATGGATGGCTCCTCGGGTACATACTGGCCAGTGAACTCAGACCAGGCCGTGCCTGAGCTTGATTTGGGCTGGCCTCTGACCTTCGGCTTCCAGGGCACCGAGGTGACCACCTTGGTGCAGCCACCGCCCCCCGACAGCCCCAGTATCAAGGATGAGGCCCGCAGGATGATCCGTTCCGCCCAGCAGGTGGTGGCCGTGGTGATGGACATGTTCACTGATGTGGACCTGCTCAGCGAAGTGCTGGAGGCCGCGGCCCGTCGGGTCCCAGTCTACATCCTGCTGGATGAGATGAACGCGCAGCACTTCCTGGACATGGCCGACAAGTGCCGTGTCAACCTGCACCACGTGGATTTCCTGCGCGTACGGACTGTGGCGGGCCCCACCTACTACTGCCGCACTGGGAAGTCCTTCAAGGGCCACGTCAAGGAGAAGTTCCTGCTGGTGGACTGTGCCGTGGTGATGAGTGGGAGCTACAGCTTCATGTGGTCCTTTGAGAAGATCCACCGCAGCCTGGCGCACGTGTTCCAAGGAGAGCTGGTCTCCAGCTTCGACGAGGAGGCCCGCATCCTCTTCGCGCAGTCCGAGCCGCTTGTGCCCTCGGCCGCGGCCCTGGCCCGCATGGACGCCTATGCCCTGGCTCCGTATGCCGGGGCCGGGCCTCTCGTGGGCGTCCCTGGGGTCGGGGCGCCAACCCCCTTCTCCTTCCCTAAACGAGCGCACCTCCTGTTCCCGCCACCCCGGGAAGAGGGCCTGGGCTTCCCCTCCTTCCTCGACCCGGACCGCCACTTCCTGTCGGCCTTCCGCCGGGAGGAGCCGCCGCGGATGCCGGGGGGCGCGCTGGAACCGCACGCGGGGCTGCGGCCACTCTCGCGGCGCCTGGAGGCCGAGGCCGGGCCGGCTGGGGAGCTCGCGGGCGCGCGGGGCTTCTTCCAGGCGCGGCACCTGGAGATGGACGCCTTCAAGCGGCACAGCTTCGCGACCGAGGGCGCGGGCGCCGTGGAGAACTTCGCGGCTGCGCGGCAGGTGTCGCGGCAGACGTTCCTCAGCCACGGCGACGACTTCCGCTTCCAGACCAGCCACTTCCACCGTGACCAGCTCTACCAGCAGCAGTACCAGTGGGACCCGCAGCTCACGCCGGCGCGCCCGCAAGGCCTGTTCGAGAAGCTTCGCGGGGGCCGCGCGGGTTTCGCGGACCCGGATGACTTCACCCTGGGCGCCGGGCCCCGCTTCCCGGAGCTCGGACCCGACGGGCACCAGCGGCTGGACTACGTGCCGTCCAGCGCGTCCCGCGAGGTGCGCCACGGCTCGGACCCCGCCTTCGCGCCCGGACCCCGCGGCCTGGAGCCCAGCGGAGCCCCGCGCCCCAACCTGACCCAGCGCTTCCCATGCCAGGCCGCGGCGAGGCCGGGCCCAGACCCCGCTCCCGAGGCGGAGCCGGAGCGCAGGGGCGGGCCCGAGGGGCGGGCAGGGCTGCGGCGCTGGCGTTTGGCCTCCTACTTGAGCGGCTGCCACGGCGAGGATGGGGGCGACGACGGCCTACCGGCGCCCATGGAAGCGGAGGCTTACGAAGACGACGTGCTGGCTCCCGGGGGCCGGGCACCTGCCGGCGACCTGCTCCCCTCGGCCTTCCGCGTCCCAGCAGCCTTCCCCACCAAGGTCCCGGTGCCAGGCCCGGGCAGCGGCGGCAACGGCCCAGAGCGCGAGGGCCCGGAGGAGCCTGGCCTGGCCAAGCAGGACTCATTCCGCTCGCGCCTGAACCCCCTGGTCCAGCGCAGCTCCAGGCTGCGCTCCTCGCTCATCTTCAGCACGTCACAGGCCGAGGGCGCGGCCGGGGCTGCGGCGGCCACTGAGAAGGTGCAGCTGCTGCACAAGGAGCAGACGGTCAGCGAGACGCTGGGGCCCGGCGGAGAGGCCGTGCGCTCCGCGGCTTCCACCAAGGTGGCGGAGCTGCTGGAGAAGTACAAGGGCCCAGCCCGTGATCCCGGCGGCGGCGCGGGCGCCATCACCGTTGCCAGCCACAGCAAGGCCGTCGTGTCCCAGGCGTGGCGGGAAGAGGTGGCGGCCCCAGGTGCCGTGGGGGGCGAGCGCCGCAGCCTCGAGAGCTGCCTGCTGGACCTGCGCGACTCCTTTGCACAGCAGCTGCACCAGGAGGCGGAGCGGCAGCCGGGAGCCGCGTCGCTCACCGCGGCGCAGCTGCTCGACACACTGGGCCGGAGCGGCTCCGACCGCCTGCCTTCCCGCTTCCTCTCTGCCCAGAGCCACTCAACGTCCCCGCAAGGGCTGGACAGCCCTCTGCCGCTGGAAGGGTCCGGAGCGCACCAGGTGCTCCATAATGAGTCAAAAGGGAGCCCCACCTCGGCTTACCCTGAGCGGAAGGGGAGCCCCACGCCTGGGTTTTCCACTCGAAGAGGAAGTCCAACTACAGGATTTATCGAGCAGAAGGGGAGCCCCACCTCAGCCTACCCCGAGCGCAGGGGTAGTCCGGTGCCCCCCGTGCCGGAGCGCAGGAGCAGTCCGGTGCCCCCCGTGCCGGAGCGCAGGGGCAGCCTCACCCTTACCATCTCCGGGGAGTCCCCGAAGGCCGGGCCCGCGGAGGAGGGGCCGAGCGGCCCCATGGAAGTCCTGCGCAAAGGCTCCTTGCGTCTTAGGCAGCTGCTGAGCCCCAAGGGCGAGCGGCGCATGGAGGATGAGGGTGGCTTCCCAGTGCCGCAGGAGAACGGCCAACCCGAGAGCCCGCGGCGTCTGTCACTGGGCCAGGGTGACAGCACGGAGGCTGCCACAGAAGAGCGGGGTCCGCGGGCGCGCCTGTCCTCAGCCACGGCCAACGCCTTGTACAGCAGCAACCTTCGGGATGACACGAAGGCCATTCTGGAGCAGATCAGTGCCCACGGCCAGAAGCACCGTGCGGTCCCTGCCCCGAGCCCCGGCCCGACCCACAACAGCCCCGAGCTAGGCCGTCCACCGGCTGCTGGCGTCCTGGCCCCAGATATGTCCGACAAGGACAAGTGTTCAGCCATCTTCCGCTCGGACAGCTTGGGGACCCAGGGCCGGCTGAGCCGCACGCTGCCAGCCAGCGCGGAGGAGCGCGATCGGCTGCTGCGCCGCATGGAGAGCATGCGCAAGGAGAAGCGCGTGTACAGCCGCTTCGAGGTCTTCTGCAAGAAAGAGGAGGCCAGCAGCCCTGGGGCAGGGGAAGGCCCCGCGGAGGAGGGCACCAGGGACAGCAAGGTGGGCAAGTTCGTGCCCAAGATCCTGGGCACGTTCAAAAGCAAGAAGTGAGCGGCCGC

Amino Acid Sequence

MARRSQSSSQGDNPLAPGYLPPHYKEYYRLAVDALAEGGSEAYSRFLATE
GAPDFLCPEELEHVSRHLRPPQYVTREPPEGSLLDVDMDGSSGTYWPVNS
DQAVPELDLGWPLTFGFQGTEVTTLVQPPPPDSPSIKDEARRMIRSAQQV
VAVVMDMFTDVDLLSEVLEAAARRVPVYILLDEMNAQHFLDMADKCRVNL
HHVDFLRVRTVAGPTYYCRTGKSFKGHVKEKFLLVDCAVVMSGSYSFMWS
FEKIHRSLAHVFQGELVSSFDEEARILFAQSEPLVPSAAALARMDAYALA
PYAGAGPLVGVPGVGAPTPFSFPKRAHLLFPPPREEGLGFPSFLDPDRHF
LSAFRREEPPRMPGGALEPHAGLRPLSRRLEAEAGPAGELAGARGFFQAR
HLEMDAFKRHSFATEGAGAVENFAAARQVSRQTFLSHGDDFRFQTSHFHR
DQLYQQQYQWDPQLTPARPQGLFEKLRGGRAGFADPDDFTLGAGPRFPEL
GPDGHQRLDYVPSSASREVRHGSDPAFAPGPRGLEPSGAPRPNLTQRFPC
QAAARPGPDPAPEAEPERRGGPEGRAGLRRWRLASYLSGCHGEDGGDDGL
PAPMEAEAYEDDVLAPGGRAPAGDLLPSAFRVPAAFPTKVPVPGPGSGGN
GPEREGPEEPGLAKQDSFRSRLNPLVQRSSRLRSSLIFSTSQAEGAAGAA
AATEKVQLLHKEQTVSETLGPGGEAVRSAASTKVAELLEKYKGPARDPGG
GAGAITVASHSKAVVSQAWREEVAAPGAVGGERRSLESCLLDLRDSFAQQ
LHQEAERQPGAASLTAAQLLDTLGRSGSDRLPSRFLSAQSHSTSPQGLDS
PLPLEGSGAHQVLHNESKGSPTSAYPERKGSPTPGFSTRRGSPTTGFIEQ
KGSPTSAYPERRGSPVPPVPERRSSPVPPVPERRGSLTLTISGESPKAGP
AEEGPSGPMEVLRKGSLRLRQLLSPKGERRMEDEGGFPVPQENGQPESPR
RLSLGQGDSTEAATEERGPRARLSSATANALYSSNLRDDTKAILEQISAH
GQKHRAVPAPSPGPTHNSPELGRPPAAGVLAPDMSDKDKCSAIFRSDSLG
TQGRLSRTLPASAEERDRLLRRMESMRKEKRVYSRFEVFCKKEEASSPGA
GEGPAEEGTRDSKVGKFVPKILGTFKSKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28687
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent changes at Pro375 and Ala425 compared to NCBI ref. Parental 33769
• Low Temp: No