cDNA Clone FAM83G FAM83H

• Plasmid: pcDNA5D-FRT/TO GFP-FAM83G (M1-V310)-FAM83H (V285-K1179)
• Parent Plasmid: pcDNA5D-FRT/TO GFP
• Expressed: GFP-FAM83G (M1-V310)-FAM83H (V285-K1179)
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGGCCTTCTCTCAGGTGCAGTGTCTGGACGACAACCATGTGAA
CTGGCGCTCCAGCGAGTCCAAGCCTGAGTTCTTCTACAGCGAGGAGCAGC
GGCTGGCGCTGGAGGCCCTGGTGGCCCGCGGCCGGGACGCCTTCTACGAG
GTGCTCAAGCGGGAGAACATCCGAGACTTCCTCTCGGAGCTGGAGCTCAA
GCGCATCCTGGAGACCATCGAGGTGTACGACCCGGGCTCTGAGGACCCTC
GGGGCACGGGCCCCTCTCAGGGGCCCGAGGACAATGGGGTCGGCGACGGC
GAGGAAGCCAGCGGGGCGGATGGGGTCCCCATCGAGGCCGAGCCGCTGCC
CTCCCTGGAGTACTGGCCCCAGAAGTCGGACCGCTCCATCCCGCAGCTGG
ACCTGGGCTGGCCCGACACCATCGCCTACCGCGGCGTGACCCGGGCTAGC
GTCTACATGCAGCCCCCCATAGACGGGCAGGCCCACATCAAAGAGGTGGT
GCGGAAGATGATCAGCCAGGCACAGAAGGTGATAGCTGTGGTCATGGACA
TGTTCACCGACGTGGACATCTTCAAGGACCTGCTGGACGCCGGCTTCAAG
AGGAAAGTGGCCGTGTACATCATCGTGGATGAGAGTAACGTCAAGTACTT
CCTGCACATGTGTGAGCGGGCCTGCATGCACCTGGGGCACCTCAAGAATC
TCAGAGTGCGGAGCAGCGGGGGAACTGAGTTCTTCACGCGGTCGGCAACC
AAGTTCAAGGGTGCCCTGGCCCAGAAGTTCATGTTTGTGGATGGAGACCG
GGCTGTGTGCGGCTCCTACAGCTTCACGTGGTCGGCCGCGCGGACGGACC
GGAATGTGATCTCTGTGCTGTCTGGCCAGGTGGTGGAGATGTTTGACCGG
CAGTTCCAGGAGCTGTACCTCATGTCACACAGTGTGGTGCCCTCGGCCGC
GGCCCTGGCCCGCATGGACGCCTATGCCCTGGCTCCGTATGCCGGGGCCG
GGCCTCTCGTGGGCGTCCCTGGGGTCGGGGCGCCAACCCCCTTCTCCTTC
CCTAAACGAGCGCACCTCCTGTTCCCGCCACCCCGGGAAGAGGGCCTGGG
CTTCCCCTCCTTCCTCGACCCGGACCGCCACTTCCTGTCGGCCTTCCGCC
GGGAGGAGCCGCCGCGGATGCCGGGGGGCGCGCTGGAACCGCACGCGGGG
CTGCGGCCACTCTCGCGGCGCCTGGAGGCCGAGGCCGGGCCGGCTGGGGA
GCTCGCGGGCGCGCGGGGCTTCTTCCAGGCGCGGCACCTGGAGATGGACG
CCTTCAAGCGGCACAGCTTCGCGACCGAGGGCGCGGGCGCCGTGGAGAAC
TTCGCGGCTGCGCGGCAGGTGTCGCGGCAGACGTTCCTCAGCCACGGCGA
CGACTTCCGCTTCCAGACCAGCCACTTCCACCGTGACCAGCTCTACCAGC
AGCAGTACCAGTGGGACCCGCAGCTCACGCCGGCGCGCCCGCAAGGCCTG
TTCGAGAAGCTTCGCGGGGGCCGCGCGGGTTTCGCGGACCCGGATGACTT
CACCCTGGGCGCCGGGCCCCGCTTCCCGGAGCTCGGACCCGACGGGCACC
AGCGGCTGGACTACGTGCCGTCCAGCGCGTCCCGCGAGGTGCGCCACGGC
TCGGACCCCGCCTTCGCGCCCGGACCCCGCGGCCTGGAGCCCAGCGGAGC
CCCGCGCCCCAACCTGACCCAGCGCTTCCCATGCCAGGCCGCGGCGAGGC
CGGGCCCAGACCCCGCTCCCGAGGCGGAGCCGGAGCGCAGGGGCGGGCCC
GAGGGGCGGGCAGGGCTGCGGCGCTGGCGTTTGGCCTCCTACTTGAGCGG
CTGCCACGGCGAGGATGGGGGCGACGACGGCCTACCGGCGCCCATGGAAG
CGGAGGCTTACGAAGACGACGTGCTGGCTCCCGGGGGCCGGGCACCTGCC
GGCGACCTGCTCCCCTCGGCCTTCCGCGTCCCAGCAGCCTTCCCCACCAA
GGTCCCGGTGCCAGGCCCGGGCAGCGGCGGCAACGGCCCAGAGCGCGAGG
GCCCGGAGGAGCCTGGCCTGGCCAAGCAGGACTCATTCCGCTCGCGCCTG
AACCCCCTGGTCCAGCGCAGCTCCAGGCTGCGCTCCTCGCTCATCTTCAG
CACGTCACAGGCCGAGGGCGCGGCCGGGGCTGCGGCGGCCACTGAGAAGG
TGCAGCTGCTGCACAAGGAGCAGACGGTCAGCGAGACGCTGGGGCCCGGC
GGAGAGGCCGTGCGCTCCGCGGCTTCCACCAAGGTGGCGGAGCTGCTGGA
GAAGTACAAGGGCCCAGCCCGTGATCCCGGCGGCGGCGCGGGCGCCATCA
CCGTTGCCAGCCACAGCAAGGCCGTCGTGTCCCAGGCGTGGCGGGAAGAG
GTGGCGGCCCCAGGTGCCGTGGGGGGCGAGCGCCGCAGCCTCGAGAGCTG
CCTGCTGGACCTGCGCGACTCCTTTGCACAGCAGCTGCACCAGGAGGCGG
AGCGGCAGCCGGGAGCCGCGTCGCTCACCGCGGCGCAGCTGCTCGACACA
CTGGGCCGGAGCGGCTCCGACCGCCTGCCTTCCCGCTTCCTCTCTGCCCA
GAGCCACTCAACGTCCCCGCAAGGGCTGGACAGCCCTCTGCCGCTGGAAG
GGTCCGGAGCGCACCAGGTGCTCCATAATGAGTCAAAAGGGAGCCCCACC
TCGGCTTACCCTGAGCGGAAGGGGAGCCCCACGCCTGGGTTTTCCACTCG
AAGAGGAAGTCCAACTACAGGATTTATCGAGCAGAAGGGGAGCCCCACCT
CAGCCTACCCCGAGCGCAGGGGTAGTCCGGTGCCCCCCGTGCCGGAGCGC
AGGAGCAGTCCGGTGCCCCCCGTGCCGGAGCGCAGGGGCAGCCTCACCCT
TACCATCTCCGGGGAGTCCCCGAAGGCCGGGCCCGCGGAGGAGGGGCCGA
GCGGCCCCATGGAAGTCCTGCGCAAAGGCTCCTTGCGTCTTAGGCAGCTG
CTGAGCCCCAAGGGCGAGCGGCGCATGGAGGATGAGGGTGGCTTCCCAGT
GCCGCAGGAGAACGGCCAACCCGAGAGCCCGCGGCGTCTGTCACTGGGCC
AGGGTGACAGCACGGAGGCTGCCACAGAAGAGCGGGGTCCGCGGGCGCGC
CTGTCCTCAGCCACGGCCAACGCCTTGTACAGCAGCAACCTTCGGGATGA
CACGAAGGCCATTCTGGAGCAGATCAGTGCCCACGGCCAGAAGCACCGTG
CGGTCCCTGCCCCGAGCCCCGGCCCGACCCACAACAGCCCCGAGCTAGGC
CGTCCACCGGCTGCTGGCGTCCTGGCCCCAGATATGTCCGACAAGGACAA
GTGTTCAGCCATCTTCCGCTCGGACAGCTTGGGGACCCAGGGCCGGCTGA
GCCGCACGCTGCCAGCCAGCGCGGAGGAGCGCGATCGGCTGCTGCGCCGC
ATGGAGAGCATGCGCAAGGAGAAGCGCGTGTACAGCCGCTTCGAGGTCTT
CTGCAAGAAAGAGGAGGCCAGCAGCCCTGGGGCAGGGGAAGGCCCCGCGG
AGGAGGGCACCAGGGACAGCAAGGTGGGCAAGTTCGTGCCCAAGATCCTG
GGCACGTTCAAAAGCAAGAAGTGAgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAFSQV
QCLDDNHVNWRSSESKPEFFYSEEQRLALEALVARGRDAFYEVLKRENIR
DFLSELELKRILETIEVYDPGSEDPRGTGPSQGPEDNGVGDGEEASGADG
VPIEAEPLPSLEYWPQKSDRSIPQLDLGWPDTIAYRGVTRASVYMQPPID
GQAHIKEVVRKMISQAQKVIAVVMDMFTDVDIFKDLLDAGFKRKVAVYII
VDESNVKYFLHMCERACMHLGHLKNLRVRSSGGTEFFTRSATKFKGALAQ
KFMFVDGDRAVCGSYSFTWSAARTDRNVISVLSGQVVEMFDRQFQELYLM
SHSVVPSAAALARMDAYALAPYAGAGPLVGVPGVGAPTPFSFPKRAHLLF
PPPREEGLGFPSFLDPDRHFLSAFRREEPPRMPGGALEPHAGLRPLSRRL
EAEAGPAGELAGARGFFQARHLEMDAFKRHSFATEGAGAVENFAAARQVS
RQTFLSHGDDFRFQTSHFHRDQLYQQQYQWDPQLTPARPQGLFEKLRGGR
AGFADPDDFTLGAGPRFPELGPDGHQRLDYVPSSASREVRHGSDPAFAPG
PRGLEPSGAPRPNLTQRFPCQAAARPGPDPAPEAEPERRGGPEGRAGLRR
WRLASYLSGCHGEDGGDDGLPAPMEAEAYEDDVLAPGGRAPAGDLLPSAF
RVPAAFPTKVPVPGPGSGGNGPEREGPEEPGLAKQDSFRSRLNPLVQRSS
RLRSSLIFSTSQAEGAAGAAAATEKVQLLHKEQTVSETLGPGGEAVRSAA
STKVAELLEKYKGPARDPGGGAGAITVASHSKAVVSQAWREEVAAPGAVG
GERRSLESCLLDLRDSFAQQLHQEAERQPGAASLTAAQLLDTLGRSGSDR
LPSRFLSAQSHSTSPQGLDSPLPLEGSGAHQVLHNESKGSPTSAYPERKG
SPTPGFSTRRGSPTTGFIEQKGSPTSAYPERRGSPVPPVPERRSSPVPPV
PERRGSLTLTISGESPKAGPAEEGPSGPMEVLRKGSLRLRQLLSPKGERR
MEDEGGFPVPQENGQPESPRRLSLGQGDSTEAATEERGPRARLSSATANA
LYSSNLRDDTKAILEQISAHGQKHRAVPAPSPGPTHNSPELGRPPAAGVL
APDMSDKDKCSAIFRSDSLGTQGRLSRTLPASAEERDRLLRRMESMRKEK
RVYSRFEVFCKKEEASSPGAGEGPAEEGTRDSKVGKFVPKILGTFKSKK*

• Vector Type: Mammalian
• Antibiotic: Amp; Kan
• DU Number: DU28688
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Chimeric fusion of FAM83G M1-V310 DUF domain on FAM83H non DUF.; Parental 41455
• Low Temp: No