cDNA Clone FAM83H

• Plasmid: pcDNA5D-FRT/TO GFP-FAM83H (M1-L284)-NLS
• Parent Plasmid: pcDNA5D-FRT/TO GFP
• Expressed: GFP-FAM83H (M1-L284)-NLS
• Genbank: NM_198488.3
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGGCCCGTCGCTCTCAGAGCTCCTCGCAGGGGGACAACCCACT
GGCACCCGGGTACCTGCCGCCTCACTACAAAGAGTACTACCGCCTGGCGG
TGGATGCACTGGCCGAGGGTGGCTCGGAGGCCTACAGCCGCTTCCTCGCT
ACCGAGGGGGCACCAGACTTCCTGTGCCCTGAAGAGCTGGAACATGTGAG
CCGACACCTTCGGCCTCCGCAGTATGTTACCCGAGAGCCACCTGAAGGCA
GCCTTCTCGACGTGGACATGGATGGCTCCTCGGGTACATACTGGCCAGTG
AACTCAGACCAGGCCGTGCCTGAGCTTGATTTGGGCTGGCCTCTGACCTT
CGGCTTCCAGGGCACCGAGGTGACCACCTTGGTGCAGCCACCGCCCCCCG
ACAGCCCCAGTATCAAGGATGAGGCCCGCAGGATGATCCGTTCCGCCCAG
CAGGTGGTGGCCGTGGTGATGGACATGTTCACTGATGTGGACCTGCTCAG
CGAAGTGCTGGAGGCCGCGGCCCGTCGGGTCCCAGTCTACATCCTGCTGG
ATGAGATGAACGCGCAGCACTTCCTGGACATGGCCGACAAGTGCCGTGTC
AACCTGCACCACGTGGATTTCCTGCGCGTACGGACTGTGGCGGGCCCCAC
CTACTACTGCCGCACTGGGAAGTCCTTCAAGGGCCACGTCAAGGAGAAGT
TCCTGCTGGTGGACTGTGCCGTGGTGATGAGTGGGAGCTACAGCTTCATG
TGGTCCTTTGAGAAGATCCACCGCAGCCTGGCGCACGTGTTCCAAGGAGA
GCTGGTCTCCAGCTTCGACGAGGAGTTCCGCATCCTCTTCGCGCAGTCCG
AGCCGCTTccaccgaagaagaagcgcaagtgaGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMARRSQ
SSSQGDNPLAPGYLPPHYKEYYRLAVDALAEGGSEAYSRFLATEGAPDFL
CPEELEHVSRHLRPPQYVTREPPEGSLLDVDMDGSSGTYWPVNSDQAVPE
LDLGWPLTFGFQGTEVTTLVQPPPPDSPSIKDEARRMIRSAQQVVAVVMD
MFTDVDLLSEVLEAAARRVPVYILLDEMNAQHFLDMADKCRVNLHHVDFL
RVRTVAGPTYYCRTGKSFKGHVKEKFLLVDCAVVMSGSYSFMWSFEKIHR
SLAHVFQGELVSSFDEEFRILFAQSEPLPPKKKRK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28709
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: FAM83H DUF domain with C-terminal nuclear localisation signal (NLS). Direct ligation of purified PCR product. Parental 41455.
• Low Temp: No